TCF1/TCF7 (C63D9) Rabbit mAb 2203S from Cell Signaling Technology

Product Specs
ItemTCF1/TCF7 (C63D9) Rabbit mAb
CompanyCell Signaling Technology
Price $366.00 Inquire
Catalog Number2203S
Quantity100 µl
ApplicationsW, IP, IHC-P, IF-F, IF-IC, FC-FP, ChIP
ReactivityH, M
TargetTCF7
Molecular Weight48, 50 kDa
HostRabbit
NCBI Gene AliasesTCF-1
ClonalityMonoclonal
NCBI Full Gene Nametranscription factor 7
CloneC63D9
Research AreaDevelopmental Biology

Questions and Answers

Can this antibody be used to study TCF1/TCF7 in mouse models?

Yes, the TCF1/TCF7 (C63D9) Rabbit mAb has been shown to work effectively in mouse models, as indicated by its use in various applications including Western blotting and immunofluorescence in studies involving mouse nephron progenitor cells ().

Is this antibody suitable for use in immunohistochemistry on human tissues?

The TCF1/TCF7 (C63D9) Rabbit mAb has been utilized in immunohistochemistry, demonstrating its capability to detect TCF1/TCF7 protein in human tissues, as referenced in multiple studies (, ).

How does this antibody perform in ChIP assays?

The TCF1/TCF7 (C63D9) Rabbit mAb has been cited in studies for its application in chromatin immunoprecipitation (ChIP) assays, where it effectively detects TCF1/TCF7 binding to DNA target sites, contributing to the understanding of Wnt signaling pathways ().

What specific applications has this antibody been validated for in the literature?

This antibody has been validated for use in several applications including Western blotting, flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, and chromatin immunoprecipitation, as indicated by its extensive use in various research articles (, , ).

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Overcoming ADC resistance in advanced colorectal cancer by dual targeting of TROP2 and PERK to suppress Wnt/β-catenin signalingCell Reports MedicineMay 19, 2026Jie Liu, Mei Li, Jianming Huang, Yujie Xia, Guanfeng Jiang, Hong’an Zhang, Jiaxin Yang, Mingfeng Jiang, Yalan Liu, Yilun Luo, et al.anti-TOP1,Proteintech,Cat#20705-1-AP; RRID:AB_10695762 Rabbit polyclonal anti-TCF1 (C63D9),Cell Signaling Technology,Cat#2203; RRID:AB_2199302 HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H + L),Proteintech,Cat#RGAR001; RRID:AB_3073505 HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L

(2) Targeting Mettl8-Tcf1 axis promotes CD8+ TPEX differentiation and antitumor immunityJ Exp MedMay 4, 2026Jiaxi Song, Dan Cui, Jiabao Wang, Xuewei Qi, Jiaxin Ma, Qing Liu, Jing Yang, Haoyu Yu, Lilin Ye, Qizhao Huang, et al.inhibitor cocktail; 0.05% digitonin) and then incubated overnight with anti-Tcf1 (1:50, C63D9, cat. no. 2203; Cell Signaling Technology), anti-H3K27ac (1:50, cat. no. ab4729; Abcam), or anti-Flag (1:50, D6W5B,

(3) Homeostatic mature dendritic cells instruct fibroblast specialization via Notch2 signaling to establish T cell nichesImmunityApril 23, 2026Nagham Alouche, Hélène Cannelle, Stéphanie Favre, Marija Jokić, José A. Villegas, Sandra Maréchal, Clint De Nolf, Maeva Delacrétaz, Alexandros Sifis, Chloé Chapuis, et al.ZET",BioLegend,Cat# 148212; RRID:AB_2564367 "Rabbit anti TCF1, clone: C63D9",Cell Signaling Technology,Cat# 2203; RRID:AB_2199302 "Rat anti CD127/IL7Ra Biotin, clone: A7R34",BioLegend,Cat# 135006; RRID:AB_2126118 "Armenian hamster

(4) Expansion and mobilization of virus-specific stem-like CD8 T cells in chronic viral infection after treatment with a long-acting IL-7, NT-I7Cell ReportsApril 1, 2026Judong Lee, Christina Niavi, Eunseon Ahn, Haydn T. Kissick, Rajesh M. Valanparambil, Tahseen H. Nasti, Donald J. McGuire, Daniel Hua, Rebecca Obeng, Alexandra A. Wolfarth, et al.(clone:29F.1A12),Biolegend,Cat#:135225; RRID:AB_2563680 anti-TCF1 (clone:C63D9),Cell signaling,Cat#:2203S; RRID:AB_2199302 anti-Tim-3 BV421 (clone:RMT3-23),Biolegend,Cat#:119723; RRID:AB_2616908 anti-TNF

(5) HOXA9 orchestrates EMT and metastasis in oral cancer via transcriptional activation of vimentin and β-catenin signalingCell Death & DiseaseMarch 28, 2026U. Sangeetha Shenoy, Divya Adiga, Dhanraj Salur Basavarajappa, Naveena A. N. Kumar, Adarsh Kudva, Ajaikumar B. Kunnumakkara, Keith D. Hunter, Shama Prasada Kabekkodu, Raghu Radhakrishnansc-81291, Santacruz, USA), CTNNB1 (8480T), nonphospho-CTNNB1 (8814S), SNAI2 (9585T), C-Myc (5605 T), TCF-1 (2203T), CASP9 (9502T), CDH1 (3195 T), ACTB (4967S), phospho-AKT (9271S), Total-AKT (9272), phospho-ERK1/2

(6) Ultra-precision deconvolution of spatial transcriptomics decodes immune heterogeneity and fate-defining programs in tissuesNature CommunicationsMarch 20, 2026Yin Xu, Zurui Huang, Yawei Zhang, Minghui Gong, Zhenghang Wang, Peijin Guo, Feifan Zhang, Jing Yang, Guanghao Liang, Lihui Dong, et al.Cat# ab256458, clone EPR22851-145, RRID:AB_3096210; 1:400), and TCF1 (Cell Signaling Technology Cat# 2203 T, clone C63D9; 1:200) were sequentially applied at 4 °C overnight or at room temperature for 1 h. The

(7) ITK-targeted immune remodeling enhanced the efficacy of anti-CD19 CAR-T cell therapyCell Death DiscoveryMarch 6, 2026Zhenjun Li, Liangcheng Lv, Xiaoyu Yao, Zhiwei Feng, Yan Xie, Kecheng Li, Feifei Qi, Mei Yang, Jingwen Wang, Tao Pan, et al.Shanghai, China) was used to detect CAR-T cells. Phospho-Ser1248-PLCγ1 (#8713), PLCγ1 (#5690), TCF1 (#2203), NR4A (#3960), NFAT1 (#5861), Tox (#36778), BATF (#8638), TNF-a (#3707), LAG3 (#15372) and GAPDH (#5174)

(8) Targeting the Mapk13‐Tcf1‐Slc7a5 Axis via One‐Carbon Metabolic Regulation to Prevent Chronic Allograft VasculopathyAdvanced ScienceMarch 1, 2026Wang Yi, Di Wu, Jing Liu, Shi Chen, Liu Song, Bin Xie, Aini Xie, Peixiang Lan, Zhishui ChenThe primary antibodies used were as follows: CD4 (cat# 48274, CST), CD8 (cat# 85336, CST), Tcf1 (cat# 2203s, CST), CD4 (cat# 25229, CST), CD8 (cat# 98941, CST), CD11b (cat# 93169, CST), and CD11c (cat# 97585,

(9) Tumour–brain crosstalk restrains cancer immunity via a sensory–sympathetic axisNatureFebruary 26, 2026Haohan K. Wei, Chuyue D. Yu, Bo Hu, Xing Zeng, Hiroshi Ichise, Liang Li, Yu Wang, Ruiqi L. Wang, Ronald N. Germain, Rui B. Chang, et al.505806, BioLegend), FITC-Ly6C (AL-2T, 1:1,000, 127645, BioLegend), Alexa Fluor 488-TCF1 (C63D9, 1:600, 2203S, Cell Signaling Technology), PE/CF594-CD45 (30-F11, 1:200, 562420, BD), PE/Cy5-PD-L1 (10F.9G2, 1:2,400,

(10) High efficiency CRISPR knock-in demonstrates that TCF1 is insufficient to reverse T cell exhaustionNature CommunicationsFebruary 17, 2026Maria N. de Menezes, Amanda X. Y. Chen, Nihali Kulkarni, Shienny Sampurno, Nicole Y. L. Saw, Kah Min Yap, Iván Pérez-Núñez, Sara Roth, Christian Deo T. Deguit, Brandon Haugen, et al.Scientific) and stained with rabbit anti-mouse TCF1 mAb (clone C63D9, Cell Signaling Technology, Cat. no. 2203S, 1:400) detected with a polyclonal secondary anti-rabbit IgG AlexaFluor594 Ab (ThermoFisher Scientific,

(11) Immunophenotyping TCF1-expressing TILs: spatial profiling and prognostic value in operable non-small cell lung cancerFrontiers in ImmunologyJanuary 22, 2026Konstantinos Ntostoglou, Georgios Christodoulopoulos, Katie Stoker, Jean Descarpentrie, Anastasia Xagara, Dora Chatzidaki, Vasiliki Anastasopoulou, Ilias P. Nikas, Argyro Ioanna Ieronimaki, Lucy Booth, et al.4B12, IS649, DAKO, ready to use), anti-CD79α (JCB117, DAKO, ready to use), anti-TCF1 (clone C63D9, 2203, Cell Signaling, 1:50), anti-PD1 (NAT105, Cell Marque, 1:50) and anti-PDL1 (clone SP263, Roche). PD-L1

(12) Lymphotoxin-driven cancer cell eradication by tumoricidal CD8 + TILbioRxivJanuary 16, 2026Hongyan Xie, Aiping Jiang, Aonkon Dey, Joseph W. Dean, Jonathan J. Perera, Neal P. Smith, Alex C. Y. Chen, Seth Anderson, Angelina M. Cicerchia, Yi Sun, et al.using the Opal 7-color Automation IHC Kit (NEL821001KT, Akoya Biosciences): TCF1/7 (Cell Signaling, #2203) with Opal 690, CD8 (Leica Biosystems NCL-L-CD8–4B11) with Opal 480, PD1 (Abcam ab137132) with Opal

(13) CD82-associated exhausted CD8+ T cells define prognosis and immunotherapy resistance in colon cancerFrontiers in ImmunologyJanuary 13, 2026Jingfeng Zhang, Hengwei Cui, Shaoxian Wu, Hongwei Shi, Haitao Wang, Jingting JiangD5D5R™, 1:100 dilution, Catalog No.: 45208S, CST, USA), TCF1 (clone C63D9, 1:200 dilution, Catalog No.: 2203S, CST, USA), PD-1 (clone UMAB199, 1:100 dilution, Cat. No.: ZM0381, ZSGB-BIO, China), and PANCK (clone

(14) Linavonkibart and pembrolizumab in immune checkpoint blockade-resistant advanced solid tumors: a phase 1 trialNature MedicineJanuary 13, 2026Timothy A. Yap, Randy F. Sweis, Ulka Vaishampayan, Deepak Kilari, Justin F. Gainor, Meredith McKean, Minal Barve, Ahmad A. Tarhini, Bruno Bockorny, Guru Sonpavde, et al.(Abcam; cat. ab52587, clone NAT105, dilution 1:100), T cell factor 1 (Cell Signaling Technology; cat. 2203, clone C63D9, dilution 1:150) and CD8 (Dako; cat. M7103, clone C8/144B, dilution 1:25). Treg cells were

(15) Oncolytic adenovirus delivery of neoantigens sensitizes low-mutation tumors to anti-PD-1 therapy and prevents metastasisSignal Transduct Target TherDecember 23, 2025Ke-Yu Shen, Shi-Zhe Yu, Ying-Han Su, Sun-Zhe Xie, Chen Zhang, Hao Xu, SamI Yang, Tian-Tian Zou, Yan Fu, Hao Wang, et al.rabbit PolymAb (ABclonal, China; A26620PM, 1:500), TCF1/TCF7 rabbit mAb (Cell Signaling Technology, USA; 2203, 1:500), and anti-CD8 alpha antibody (Abcam, USA; ab237709, 1:1000). Statistical analysis Statistical

(16) Erythropoietin receptor on cDC1s dictates immune toleranceNatureDecember 10, 2025Xiangyue Zhang, Christopher S. McGinnis, Guotao Yu, Sijie Chen, Pingping Zheng, Christian M. Schürch, Kamir J. Hiam-Galvez, Nathan E. Reticker-Flynn, Wenhui Guo, Winnie Yao, et al.(13G3–19D), 17–1508-82, eBioscience), FOXP3-FITC (FJK-16s, 11–5773-82, eBioscience, 1:100), TCF1/TCF7 (C63D9, 2203S, Cell Signaling Technology), AF488 Donkey anti-rabbit IgG (Poly4064, 406416, BioLegend), T-bet-APC (eBio4B10

(17) Therapeutic radiation drives leptomeningeal dissemination of medulloblastoma through an innate immune processDevelopmental CellNovember 3, 2025Carolina Nör, Kaitlin Kharas, Alex Rasnitsyn, Maria C. Vladoiu, Nam Woo Cho, Jacob S. Young, Felipe Nör, Ncedile Mankahla, Joonas Haapasalo, Kristiina Nordfors, et al.CD83,Biolegend,121502 158GdB220,Biolegend,103202 159TbPD-1,Biolegend,135202 160GdNK1.1,Biolegend,108702 161DyT-bet,Biolegend,644825 162DyTCRgd,Biolegend,118101 163DyCD62L,R&D Systems,MAB5761 164DyTCF1,Cell Signaling,2203S 165HoCD69,Biolegend,104502 166ErFcER1a,Biolegend,134321 167ErFoxp3,Thermo Fisher,14–4771-80 168ErCD86,Biolegend,105002 169TmF4/80,Biolegend,123143 170ErCD115,Biolegend,135521 171YbCD44,Fluidigm,3171003B 172YbTIM3,Biolegend,119702 173YbCD19,Biolegend,115547 174YbiNOS,Thermo Fisher

(18) Bi-specific T cell-engaging antibody triggers protective immune memory and glioma microenvironment remodeling in immune-competent preclinical modelsJournal for immunotherapy of cancerOctober 28, 2025Zannikou M, Duffy JT, Procissi D, Najem H, Levine RN, Thakur A, Hambardzumyan D, Lee-Chang C, Leoni L, Horbinski CM, et al.1/500); IFN-γ (Bioss, BS-0480R, polyclonal, dilution 1/100); TCF1 (Cell Signaling, Clone C63D9, cat#2203, dilution 1/500); IL13RA2 (Cell Signaling, E7U7B, 85677, dilution 1/200). The detailed description of

(19) The transcriptional repressor BLIMP1 enforces TCF-1-dependent and -independent restriction of the memory fate of CD8+ T cellsImmunityOctober 14, 2025Maegan K. Murphy, Matthew McCullen, Joshua L. Deffenbaugh, Andy Y. Chen, Joy Pai, Bence Daniel, Amir Yousif, Saravanan Raju, Sunnie Hsiung, Zhenxiao Wang, et al.(Clone:4B10),BioLegend,Cat#644823; RRID: AB_2561760 Unconjugated anti-TCF1 (Clone:C63D9),Cell Signaling,Cat#2203S; RRID: AB_2199302 APC-Cy7-conjugated anti-TCR Va2 (Clone:B20.1),BioLegend,Cat#127818; RRID: AB_10682897 "FITC-conjugated

(20) High-dose ascorbic acid selectively induces pyroptosis in LKB1-deficient lung cancer and sensitizes immunotherapyCell Reports MedicineSeptember 16, 2025Xiangyu Sun, Xiaoting Cai, Shangbiao Li, Ruozheng Pi, Zeqin Guo, Jiayu Jiang, Pinhao Wang, Jingrong Xiong, Zhuangzhuang Liu, Zixuan Rong, et al.anti-F4/80 (Abcam, #ab300421) anti-SPP1 (Abcam, #ab218237), anti-CD8 (Abcam, #ab217344), anti-TCF7(CST, # 2203T), anti-phospho-MLKL (Affinity, #AF7420), anti-Caspase 11 (Affinity, #AF5130), anti-MLKL (Proteintech,

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9/26/2022Great Anti-TCF1/TCF7 Antibody Liyong Z.A549 cells stably expressing the indicated shRNAs were lysed and cell lysates were then blotted with the indicated antibodies.The results indicated that USP20 regulated cancer cell proliferation and ... Read Review
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3/25/2021Cell Signaling Technology TCF1/TCF7 (C63D9) Rabbit mAb Review Kung-Chi KaoTo titrate the antibody, 2 x 10^6 splenocytes from C57BL/6 mouse were stained with: PerCP-Cy5.5 anti-CD8 Ab and Zombie NIR in 100 µL PBS at 4C for 30 min. Subsequently, the cells were fixed/permed ... Read Review

TCF1/TCF7 (C63D9) Rabbit mAb

TCF1/TCF7 (C63D9) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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TCF1/TCF7 (C63D9) Rabbit mAb from Cell Signaling Technology

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