Oct-4 Antibody 2750S from Cell Signaling Technology

Product Specs
ItemOct-4 Antibody
CompanyCell Signaling Technology
Price $350.00 Inquire
Catalog Number2750S
Quantity100 µl
ApplicationsW, IHC-P, IF-IC, FC-FP, ChIP
ReactivityH
TargetOct4A
HostRabbit
NCBI Gene AliasesOCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
NCBI Full Gene NamePOU class 5 homeobox 1
ClonalityPolyclonal
Research AreaDevelopmental Biology
Molecular Weight45

Questions and Answers

Can this Oct-4 antibody be used in Western blotting to detect stemness markers in cancer research?

Yes, the Oct-4 antibody has been successfully used in Western blotting to detect Oct-4 protein levels in various studies, including its role in regulating stemness genes in non-small cell lung cancer, as demonstrated in the research by Guo et al. ().

Is the Oct-4 antibody effective for immunocytochemistry applications in stem cell studies?

The Oct-4 antibody has been utilized in immunocytochemistry to visualize Oct-4 expression in stem cells, as indicated in multiple studies, including its application in characterizing cancer stem cells in breast cancer models ().

What species does this antibody react with, and is it suitable for human cell lines?

The Oct-4 antibody is specifically reactive with human (Homo sapiens) samples, making it suitable for use in experiments involving human cell lines, as noted in its product specifications.

Has this antibody been cited in studies related to cancer stem cell properties?

Yes, the Oct-4 antibody has been cited in over 215 scientific articles, including studies that explore its role in cancer stem cell properties and tumorigenesis, such as the work by Liu et al. () and Siddhart et al. ().

Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) A peripheral blood-based approach involving vimentin along with AURKA enabled efficient tracking of elusive Oct4/Sox2-expressing disseminated breast cancer stem cellsBiosci RepJune 24, 2026Debomita Sengupta, Debanjan Thakur, Elizabeth Mahapatra, Salini Das, Jayanta Chakrabarti, Sagar Sen, Sutapa Mukherjeein a −20°C freezer as input, and to the rest, antibodies, namely, Oct4 (Cell Signaling Technology, 2750S), Sox2 (Cell Signaling Technology, 23064S), and rabbit IgG isotype control (GeneTex, GTX35035), were

(2) Natural Extracts of Alnus japonica Induce BAK-Dependent Autophagy to Inhibit Liver Cancer Stem Cell TumorigenesisAntioxidants (Basel)May 29, 2026Kenly Wuputra, Yoshimasa Matsuura, Satoshi Gushiken, Hirosuke Fukuda, Ya-Han Yang, Chia-Chen Ku, Chun-Chieh Wu, Ying-Chu Lin, Yi-Chun Tsai, Deng-Chyang Wu, et al.relevant antibodies. Primary antibodies included: AHR (SC-8088; Santa Cruz, Dallas, TX, USA), OCT4 (2750S; Cell Signaling Technology, Boston, MA, USA), ABCG2 (GTX100437; GeneTex, Irvine, CA, USA), HO-1 (SC-10789;

(3) Functional impact of genetic background on variable expressivity in neurodevelopmental disordersNature CommunicationsMay 1, 2026Jiawan Sun, Serena Noss, Corrine Smolen, Venkata Hemanjani Bhavana, Deepro Banerjee, Maitreya Das, Belinda Giardine, Anisha Prabhu, David J. Amor, Kate Pope, et al.Signaling Technology); NANOG (1:500, catalog #4893 T, Cell signaling Technology); OCT4 (1:200, catalog #2750S, Cell Signaling Technology). Secondary antibodies used were: Anti-mouse IgG (H + L), F(ab’)2 Fragment

(4) Exosomes derived from galangin preconditioned mesenchymal stem cells attenuate oxidative stress induced senescence and promote geriatric wound healingScientific ReportsApril 30, 2026Su-Ying Wen, Shang-Chuan Ng, Yen-Tun Chiu, Shih-Wen Kao, Tzu-Jung Chen, Hsin-Ying Huang, Chih-Yang Huang, Wei-Wen Kuowere utilized in this study for Western blotting or immunostaining: anti-Nanog (#4903), anti-Oct4 (#2750), anti-CD9 (#13,174), anti-p-PI3K p85(Tyr458)/p55 (Tyr199) (#4228), and anti-p53 (#2524) from Cell Signaling Technology

(5) Triazine Thiols Decrease Apolipoprotein B Secretion From Hepatocytes Through Inhibition of Human Carboxylesterase 1Cellular and Molecular Gastroenterology and HepatologyApril 29, 2026Josef Blaszkiewicz, Yu-Lin Jiang, Jui-Tung Liu, Carla Martinez-Morant, Troy Dearth, Casper van Altena, Mary Paige Lamprecht, Christiana Kappler, Mi-Hye Lee, Jennifer R. Bethard, et al.XP,IF – primary,Cell Signaling Technology,4903 Rabbit anti-OCT4,IF – primary,Cell Signaling Technology,2750 Rabbit anti-SOX2,IF – primary,Abcam,ab97959 Mouse anti-AFP,IF – primary,Sigma-Aldrich,A8452 Rabbit

(6) Targeting TYRO3 attenuates apoptotic cell–driven cancer stemness in triple negative breast cancerCell Communication and SignalingApril 23, 2026Phuong Thao Tran, Wan Seob Shim, Won-Cheol Jeong, Jaewoo Park, Myung Jun Kim, Seungseok Oh, Yong June Choi, Munkyung Choi, Miso Park, Keon Wook Kangantibodies were used: AXL (#8661), MERTK (#4319), TYRO3 (#5585), YAP/TAZ (#8418), NANOG (#4903), OCT4 (#2750), phospho-LATS1(#8654), phospho-YAP S127 (#13008) and α-tubulin (#2144) (all from Cell Signaling Technology,

(7) ALKBH5/IGF2BP1-mediated m6A demethylation of TRIM37 promotes pancreatic cancer tumorigenesis and glycolysis by mediating RBMX degradationCell Biology and ToxicologyApril 1, 2026Cheng Yan, Xuan Li, Ying Zhao, Jinhui Chen, Xinke Peng, Daichao Wu, Yabin Chang, Guodong Chencontrol vectors were purchased from GenePharma. CD133 (cat# 64,326), SOX2 (cat# 23,064), OCT4 (cat# 2750), GLUT1 (cat#12,939), HK2 (cat#2867), LDHA (cat#2012), PKM2 (cat#4053), RBMX (cat#14,794), ALKBH5 (cat#80,283)

(8) PKM2 Lactylation Promotes Colorectal Cancer Vasculogenic Mimicry and Bevacizumab Resistance by Facilitating FOSL1 Super-enhancer FormationCancer ResMarch 5, 2026Weihao Li, Jianhong Peng, Jiahua He, Leen Liao, Da Kang, Weili Zhang, Weifeng Wang, Ruowei Wang, Song Wang, Yuanbin Liao, et al.(Abcam, ab93689), anti-NANOG (Cell Signaling Technology, 4903), anti-OCT4 (Cell Signaling Technology, 2750), anti-Snail (Cell Signaling Technology, 3879), anti–E-cadherin (Cell Signaling Technology, 3195), anti-vimentin

(9) Exosomal circ0000549 promotes MNNG‑induced gastric cancer through miR‑15b‑5p/KIF1BInt J Mol MedMarch 4, 2026Zhaofeng Liang, Zihan Gao, Yue Zhang, Jiajia Song, Hui Qian, Xuezhong Xu(Cell Signaling Technology, Inc.; cat. no. 4903S; dilution: 1:1,000), OCT4 (Cell Signaling Technology, Inc; cat. no. 2750; dilution: 1:1,000), Lin28 (Cell Signaling Technology, Inc.; cat. no. 8706S; dilution: 1:1,000), SOX2

(10) Human papillomavirus16 E7 enhances cell stemness by regulating the APC2/SPIN4/β-catenin axis in cervical cancerOncogenesisFebruary 24, 2026Tao Shen, Yuejiang Ma, Tingting Wu, Zhu Cao, Peng Yi, Xiufeng Huang, Shizhou YangName,Brand,Number,Dilution rate APC2,Proteintech,13559-1-AP,1:1000 (WB)1:400 (IHC) OCT4,CST,#2750,1:1000 (WB)1:400 (IHC) SOX2,Proteintech,66411-1-Ig,1:5000 (WB)1:200 (IHC) Nanog,CST,#4903,1:2000 (WB)1:400

(11) Isogenic cortical organoids enable precision targeting of APP variant-specific pathways in Alzheimer’s diseasebioRxivFebruary 24, 2026T Grass, I M Cosacak, A Ordureau, F D Price, S Kavali, A Caldarelli, M N Qiao, B Vardarajan, Joao A. Paulo, Michele Marass, et al.used: CTIP2 (Abcam ab18465, rat), MAP2 (Lifespan Biosciences LS-C61805, chicken), OCT4 (Cell Signaling 2750 Rabbit), SOX2 (Invitrogen, 14-9811-82, rat), amyloid precursor protein (Abcam, ab32136, rabbit), PHF1

(12) PRDM1 Knockdown Promotes Ferroptosis and Sunitinib Sensitivity by Modulating the PI3K/Akt Signaling Through Inhibition of ESM1 Transcription in Renal Cell CarcinomaThe Kaohsiung Journal of Medical SciencesFebruary 16, 2026Yi‐Shuai Zhang, Xin‐Qi Pei, Ji‐Ping Sun, Chen‐Hui Ma, Xin‐Yang Wang, Xu‐Dong LiCat. No. 52455; Cell Signaling Technology), octamer‐binding transcription factor 4 (OCT4; Cat. No. 2750; Cell Signaling Technology), SRY (sex‐determining region Y)‐box 2 (SOX2; Cat. No. 2748; Cell Signaling Technology

(13) Connexin43 Deficiency Leads to Ventricular Arrhythmias by Reprogramming Proline MetabolismAdvanced ScienceJanuary 31, 2026Hangying Ying, Hangping Fan, Yunhe Wang, Ruhong Jiang, Dongsheng Cai, Hui Cheng, Hao Wang, Chenyang Jiang, Ping Liangµg/mL) or Hoechst (Solarbio, HOE 33258). The primary antibodies were OCT4 (Cell Signaling Technology, 2750S, 1:200), NANOG (Santa Cruz Biotechnology, sc‐33759, 1:200), SSEA‐4 (Abcam, ab16287, 1:200), SOX2 (Abcam,

(14) Otic organoids: A model to study spiral ganglion neuron characteristics in Tmprss3-deficiencyiScienceJanuary 16, 2026André U. Deutschmann, Lucie Pifkova, Betül Findik, Moritz Klingenstein, Anton Betz, Maksim Klimiankou, Julia Skokowa, Stefan Liebau, Ellen Reisinger, Stefanie KlingensteinLab,MAP MYO7A,Proteus bioscience,25-6790 NANOG,Cell Signaling,4903 NF200,Sigma,N4142 OCT4,Cell Signaling,2750 PAX8,Abcam,ab97477 POU4F1,Millipore,MAB1585 PRH,Santa Cruz,Sc-377093 PROX1,Millippore,ABN278 S100ß,Abcam,ab52642 SOX2,Cell Signaling

(15) ENOX2 (tNOX)–Associated Stemness in Oral Cancer Cells and Its Clinical Correlation in Head and Neck TumorsAntioxidants (Basel)January 13, 2026Che-Wei Wang, Atikul Islam, Yu-Tung Shih, Chin-Fang Chang, Mu Kuan Chen, Pin Ju Chueh(1:1000, Cat#2496), anti-Nanog (1:2000, Cat#4903), anti-SOX2 (1:1000, Cat#2748), anti-Oct4 (1:1000, Cat#2750), anti-ABCG2 (1:1000, Cat#4477), anti-ALDH1 (1:1000, Cat#54135), anti-PKCδ (1:1000, Cat#2058), anti-c-Myc

(16) Tumor-associated neutrophils drive liver-specific metastatic progression in breast cancer through methionine metabolism mediated by methionine adenosyltransferase II alphaTranslational Cancer ResearchDecember 31, 2025Lifeng Huang, Shuang Zhen, Qi Wang, Yue Sun, Tian Ji, Rui Chen, Jue Wang, Qiannan Zhu, Dongwei Xu, Xiaoming Zhaanti-SOX2 (Cat# 23064, RRID: AB_2714146, Cell Signaling Technology, Danvers, MA, USA), anti-OCT4 (cat. #2750, RRID: AB_823583, Cell Signaling Technology), anti-β-catenin (cat. #9562, RRID: AB_331149, Cell Signaling Technology

(17) Sestrin3 confers resistance to recombinant human arginase in small cell lung cancer by activating Akt/mTOR/ASS1 axisPLoS OneDecember 29, 2025Zhongqiang Zhang, Zizhe Lin, Weishan Li, Binxiong Chen, Yueming Liu, Hanchao Gao, Shi Xu,Cell Signaling Technology,36671,55,1:1000 Nanog,Rabbit,Cell Signaling Technology,8822,42,1:1000 Oct-4,Rabbit,Cell Signaling Technology,2750,45,1:1000 AKT,Rabbit,Cell Signaling Technology,9272,60,1:1000 p-AKT,Rabbit,Cell Signaling Technology,4060,60,1:1000 List

(18) Gedunin Impacts Pancreatic Cancer Stem Cells Through the Sonic Hedgehog Signaling PathwayPharmaceuticals (Basel)December 22, 2025Karla Perez, Sheryl Rodriguez, Jose Barragan, Poornimadevi Narayanan, Alberto Ruiseco, Preetha Rajkumar, Nallely Ramirez, Victor Vasquez, Rajkumar Lakshmanaswamy, Ramadevi Subramani(Cell Signaling Technology, #4903S), Sox2 (Cell Signaling Technology, #3579S), Oct4 (Cell Signaling Technology, #2750S), Slug (Cell Signaling Technology, #9585S), Snail (Cell Signaling Technology, #3879S), BCL-2 (Cell Signaling Technology

(19) TYRAY-Functionalized Alginate Bioinks for 3D Bioprinting Support Stem Cell Culture and Endothelial Network FormationACS Biomaterials Science & EngineeringDecember 8, 2025Vaclav Chochola, Karolina Spustova, Josef Lavicky, Anna Golunova, Jakub Pospisil, Jana Dvořáková, Ilya Kotelnikov, Mario Kandra, Libor Streit, Ariel Szklanny, et al.5 min. Samples were blocked with 5% BSA for 1 h and stained with primary antibodies against Oct-4 (2750, Cell Signaling) and E-Cadherin (3195, Cell Signaling) overnight at 4 °C. Constructs were washed 3 times

(20) INSM1 governs a neuronal progenitor state that drives glioblastoma in a human stem cell modelNat CommunDecember 7, 2025Patrick A. DeSouza, Matthew Ishahak, Xuan Qu, Colin McCornack, Devi Annamalai, Diane D. Mao, Rajanikanth Vangipurapu, Yiwei Fu, Alexandre T. Vessoni, Ryan T. Cleary, et al.(Sigma-Aldrich #X100) for 45 min. at room temp. and incubated with Oct-4 (Cell Signaling Technology #2750S, 1:400) or Pax-6 antibodies (Cell Signaling Technology #60433S, 1:200) overnight at 4 oC. Cells were

Write a Review

(1)

1/26/2024Oct-4 Antibody Worked Great for ICC on iPSCs Grace TerryI used this antibody at 1:200 dilution to confirm OCT-4 expression in my iPSC cultures. Worked great and did not have much background. I paired it with an Alexa Fluor 488 goat anti-mouse secondary.

Oct-4 Antibody

Oct-4 Antibody from Cell Signaling Technology

Description

Questions and Answers

Cell Signaling Technology

Citations (338)

Reviews

Oct-4 Antibody from Cell Signaling Technology

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map