Goat Anti-Mouse IgM-AP 1021-04 from SouthernBiotech

Product Specs
ItemGoat Anti-Mouse IgM-AP
CompanySouthernBiotech
Price $174.00Supplier Page View Company Product Page
Catalog Number1021-04
ApplicationsWB, ELISA, ELISPOT, ICC, IHC-fr, IHC-p
ReactivityMouse (Ms)
ConcentrationLot specific
PurityPooled antisera from goats hyperimmunized with mouse IgM; purified by affinity chromatography on mouse IgM covalently linked to agarose; reacts with the heavy chain of mouse IgM; cross-adsorbed against mouse IgG and IgA for minimal reactivity
ImmunogenMouse IgM
NCBI Full Gene Nameimmunoglobulin heavy constant mu
NCBI Gene AliasesIgh-6, Igh-M, Igh6, Igm, muH
Research AreaImmunology, Secondary Antibodies, Stem Cells
Antibody TypeSecondary Antibody, Primary Antibody
ClonalityPolyclonal
FormatLiquid - 50 mM Tris/1 mM MgCl2/50% Glycerol containing < 0.1% sodium azide, pH 8.0
HostGoat
IsotypeIgG
TargetIgM
Conjugate/TagAP (Alkaline Phosphatase)
Quantity1.0 mL

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SouthernBiotech

160 Oxmoor Boulevard

Birmingham, Alabama 35209

United States

Phone: (205) 945-1774
Toll-Free U.S. and Canada: (800) 722-2255
Fax: (205) 945-8768

Company Profile

Website: http://www.southernbiotech.com

(1) A new mouse model of typhoid fever using Salmonella enterica serovar Paratyphi C as a surrogate pathogenmBioFebruary 11, 2026Hoan T. Pham, Masatomo Morita, Kohei Yamazaki, Toshihiro Endo, Satoshi Takayama, Azusa Hiyoshi, Takeshi Haneda, Renée M. Tsolis, Andreas J. Bäumler, Toshio Kodama, et al.phosphatase-conjugated goat anti-mouse Ig secondary antibodies (Southern Biotech), diluted 1:4,000 for IgM (#1021-04). Detection was performed using p-nitrophenyl phosphate substrate (Sigma-Aldrich). After 1 h of incubation,

(2) MKLN1-dependent GID4/CTLH E3 ubiquitin ligase complex assemblies are required to support B-cell antibody diversificationThe Journal of ImmunologyOctober 1, 2025Philip Barbulescu, Matthew K Wong, Leon Baronian, Pengyu Wang, Abdulmateen Aderinto, Matthias Kneussel, Alberto Martinanti-mouse Ig conjugated with alkaline phosphatase (AP) in 2.5% BSA-PBS (all from SouthernBiotech): IgM-AP (1021-04, 1:2,000 dilution); IgA-AP (1040-04, 1:1,000 dilution); IgG1-AP (1070-04, 1:2,000 dilution); IgG2b-AP

(3) B cell maturation antigen (BCMA) is dispensable for the survival of long-lived plasma cellsNat CommunAugust 2, 2025Shannon R. Menzel, Edith Roth, Jens Wittner, Stefanie Brey, Leonie Weckwerth, Jana Thomas, Thomas H. Winkler, Wolfgang Schuh, Hans-Martin Jäck, Katharina Pracht, et al.antibody. Alkaline phosphatase (AP)-coupled goat-anti-mouse IgG, IgM, or IgA (Southern Biotech., Cat.: 1021-04, 1030-04, 1040-04) were used as detection antibodies with 50 µl/well at a concentration of 0.25 µg/ml.

(4) Skin autonomous antibody production regulates host-microbiota interactionsNatureDecember 11, 2024Inta Gribonika, Victor I. Band, Liang Chi, Paula Juliana Perez-Chaparro, Verena M. Link, Eduard Ansaldo, Cihan Oguz, Djenet Bousbaine, Michael A. Fischbach, Yasmine Belkaidgoat anti-mouse IgA–AP (catalogue number 1040-04, 1:1,000), goat anti-mouse IgM–-AP (catalogue number 1021-04, 1:1,000), goat anti-mouse IgG–AP (catalogue number 1036-04, 1:1,000), goat anti-mouse IgG2b–AP (catalogue

(5) EAF2 deficiency attenuates autoimmune disease in Fas (lpr) mice by modulating B cell activation and apoptosisiScienceNovember 15, 2024Luan Y, Min Q, Zhang R, Wen Z, Meng X, Hu Z, Feng X, Yu M, Dong L, Wang JY.cell culture incubator. AP-conjugated secondary antibodies (anti-mouse IgG, 1031-04; anti-mouse IgM, 1021-04; SouthernBiotech) and BCIP/NBT substrate (C3206, Beyotime) were applied for detection. Data were acquired

(6) Arid1a-dependent canonical BAF complex suppresses inflammatory programs to drive efficient germinal center B cell responsesNature ImmunologySeptember 1, 2024Ajay Abraham, Daniela Samaniego-Castruita, Isabella Han, Prathyaya Ramesh, Mi Thao Tran, Jillian Paladino, Heather Kligfeld, Roxroy C. Morgan, Rebecca L. Schmitz, Rebecca M. Southern, et al.anti-mouse IRF4 (#48-9858-82) PE anti-mouse IL-1 beta (Pro-form) (#12-7114-82) ELISA Anti-Mouse IgM-AP (#1021-04; Southern Biotech) Anti-Mouse IgG1-AP (#1071-04; Southern Biotech) From BioXcell InVivoMAb anti-mouse/rat IL-1β

(7) Systemic prime mucosal boost significantly increases protective efficacy of bivalent RSV influenza viral vectored vaccineNPJ vaccinesJune 26, 2024Bissett C, Belij-Rammerstorfer S, Ulaszewska M, Smith H, Kailath R, Morris S, Powers C, Sebastian S, Sharpe HR, Allen ER, et al.Goat Anti-Mouse IgA-AP (Cat: 1040-04 (SouthernBiotech), diluted 1:1500), Goat Anti-Mouse IgM-AP (Cat: 1021-04 (SouthernBiotech), diluted 1:1500), Goat Anti-Mouse IgG1, Human/Bovine/Horse SP ads-AP (Cat: 1071-04

(8) In BTK, phosphorylated Y223 in the SH3 domain mirrors catalytic activity, but does not influence biological function∗†Blood AdvancesApril 23, 2024H. Yesid Estupiñán, Thibault Bouderlique, Chenfei He, Anna Berglöf, Andrea Cappelleri, Nicolai Frengen, Rula Zain, Mikael C. I. Karlsson, Robert Månsson, C. I. Edvard Smithincubated with diluted serum, then AP (alkaline phosphatase)-conjugated goat antibody to mouse IgM (1021-04; SouthernBiotech), IgG (1030-04, SouthernBiotech), IgG1 (1071-04, SouthernBiotech), IgG2b (1091-04,

(9) Memory B cell subsets have divergent developmental origins that are coupled to distinctive imprinted epigenetic statesNature ImmunologyMarch 1, 2024Derrick Callahan, Shuchi Smita, Stephen Joachim, Kenneth Hoehn, Steven Kleinstein, Florian Weisel, Maria Chikina, Mark Shlomchikwash buffer, 2x H20 protocol, and then 50 ul of goat-ant-mouse IgM-AP or IgG1-AP (Southern Biotech #1021–04 and #1071–04) in PBS with 2% Fetalplex was added to appropriate wells. Plates were incubated 1 hour

(10) Attempts to evaluate locus suicide recombination and its potential role in B cell negative selection in the mouseFrontiers in ImmunologyJune 9, 2023Nicolas Denis-Lagache, Christelle Oblet, Tiffany Marchiol, Audrey Baylet, Ophélie Têteau, Iman Dalloul, Zeinab Dalloul, Lina Zawil, Ophélie Dézé, Jeanne Cook-Moreau, et al.washing, secondary anti-mouse-IgM–alkaline phosphatase (AP)-conjugated antibodies (Southern Biotech, #1021-04) were added and incubated for 1 hour at 37°C. After final washing, ELISA were revealed with SigmaFAST™

(11) Orai3 and Orai1 mediate CRAC channel function and metabolic reprogramming in B cellseLifeFebruary 21, 2023Emrich SM, Yoast RE, Zhang X, Fike AJ, Wang YH, Bricker KN, Tao AY, Xin P, Walter V, Johnson MT, et al.anti-rabbit IgG secondary antibody (SouthernBiotech, Cat#1030–04), goat anti-rabbit IgM (SouthernBiotech, Cat#1021–04) at room temperature for 2 hr. After the addition of substrate solution, absorption was measured at

(12) Distinct roles of ORAI1 in T cell-mediated allergic airway inflammation and immunity to influenza A virus infectionScience advancesOctober 7, 2022Wang YH, Noyer L, Kahlfuss S, Raphael D, Tao AY, Kaufmann U, Zhu J, Mitchell-Flack M, Sidhu I, Zhou F, et al.with alkaline phosphatase goat anti-mouse IgM, IgG, and IgG1 secondary antibodies (SouthernBiotech, 1021-04, 1036-04, and 1071-04, respectively) at 37°C for 2 hours. After addition of substrate solution, absorption

(13) ORAI3 is dispensable for store-operated Ca2+ entry and immune responses by lymphocytes and macrophagesThe Journal of general physiologyOctober 3, 2022Wang L, Noyer L, Wang YH, Tao AY, Li W, Zhu J, Saavedra P, Hoda ST, Yang J, Feske S.were measured using AP-conjugated goat anti-mouse IgM and IgG detection antibodies (1:2,000; cat. nos. 1021-04 and 1030-04; Southern Biotech). Phosphatase substrate kit was purchased from Sigma-Aldrich (cat. no.

(14) CD36 and LC3B initiated autophagy in B cells regulates the humoral immune responseAutophagyNovember 1, 2021He C, Wang S, Zhou C, He M, Wang J, Ladds M, Lianoudaki D, Sedimbi SK, Lane DP, Westerberg LS, et al.(BioTek). Secondary antibodies for Elisa: AP-conjugated goat antibody to mouse IgM (SouthernBiochem, 1021–04), IgG (SouthernBiotech, 1030–04), IgG1 (SouthernBiotech, 1071–04), IgG2b (SouthernBiotech, 1091–04),

(15) Defining the Mechanistic Correlates of Protection Conferred by Whole-Cell Vaccination against Pseudomonas aeruginosa Acute Murine PneumoniaInfection and immunityJanuary 19, 2021Sen-Kilic E, Blackwood CB, Huckaby AB, Horspool AM, Weaver KL, Malkowski AC, Witt WT, Bevere JR, Damron FH, Barbier M.1091-04), IgG2a (SouthernBiotech; 1081-04), IgG3 (SouthernBiotech; 1100-04), IgM (SouthernBiotech; 1021-04), and IgA (SouthernBiotech; 1040-04) conjugated to alkaline phosphatase. The secondary antibodies were

(16) Neonatal Exposure to Commensal-Bacteria-Derived Antigens Directs Polysaccharide-Specific B-1 B Cell Repertoire DevelopmentImmunityJuly 14, 2020New JS, Dizon BLP, Fucile CF, Rosenberg AF, Kearney JF, King RG.,Southern Biotech,CAT NO 1030-01 "Goat polyclonal anti-Mouse IgM, alkaline phosphatase",Southern Biotech,CAT NO 1021-04 "Goat polyclonal anti-Mouse IgA, alkaline phosphatase",Southern Biotech,CAT NO 1040-04 "Goat polyclonal

(17) Conditional antibody expression to avoid central B cell deletion in humanized HIV-1 vaccine mouse modelsProceedings of the National Academy of Sciences of the United States of AmericaApril 7, 2020Tian M, McGovern K, Cheng HL, Waddicor P, Rieble L, Dao M, Chen Y, Kimble MT, Cantor E, Manfredonia N, et al.antibody (SouthernBiotech 1021-01) for capture and anti-mouse IgM-alkaline phosphatase antibody (SouthernBiotech 1021-04) for detection. Purified mouse IgM (SouthernBiotech 0101-01) served as standard. Downloaded by guest

(18) Induction of Fc-Mediated Effector Functions Against a Stabilized Inner Domain of HIV-1 gp120 Designed to Selectively Harbor the A32 Epitope RegionFrontiers in ImmunologyApril 2, 2019Maria L. Visciano, Neelakshi Gohain, Rebekah Sherburn, Chiara Orlandi, Robin Flinko, Amir Dashti, George K. Lewis, William D. Tolbert, Marzena Pazgier1091-04, 1078-04, 1103-04, respectively), IgA and IgM antibodies (SouthernBiotech cat #1040-04 and 1021-04, respectively) diluted 1:1000 in blocking buffer, were added followed by incubation for 1 h at 37°C.

(19) Variant surface glycoprotein density defines an immune evasion threshold for African trypanosomes undergoing antigenic variationNature CommunicationsDecember 1, 2017Jason Pinger, Shanin Chowdhury, F. Nina PapavasiliouSecondary antibody solutions contained 1:1000 dilutions of Goat Anti-Mouse IgM-AP (SouthernBiotech 1021-04) or Goat Anti-Mouse IgG1-AP (SouthernBiotech 1070-04). Washes were performed using PBS with 0.05% Tween

(20) Autophagy in Dendritic Cells and B Cells Is Critical for the Inflammatory State of TLR7-Mediated AutoimmunityJournal of immunology (Baltimore, Md. : 1950)February 1, 2017Weindel CG, Richey LJ, Mehta AJ, Shah M, Huber BT.and incubated at 4˚C overnight. Alkaline phosphatase–conjugated goat anti-mouse IgG (1030-04), IgM (1021-04), IgG1 (1070-04), IgG2a (1080-04), IgG2b (1090-04), IgG2c (1079-04), or IgG3 (1100-04) at 1 mg/ml were

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Goat Anti-Mouse IgM-AP

Goat Anti-Mouse IgM-AP from SouthernBiotech

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Goat Anti-Mouse IgM-AP from SouthernBiotech

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