Fig 1: Effects of hyperaldosteronism and/or hypoestrogenism on selected pro/anti-apoptotic signaling molecules in female wild type (FWT) or aldostereone synthase overexpressing (FAS) mouse hearts. (A) shows representative immunoblots of phospho-Bcl2 (phospho-Ser87), Bcl2, caspase 3 and Bax. (B–E) reported their respective quantification. The phosporylation of Bcl2 was calculated as the ratio of phosphorylated form to total form (B), and, Bcl2 (C), caspase 3 (D), and Bax (E) protein expression levels were normalized to those of GAPDH. (F) the ratio of Bcl2 to Bax indicates the balance between pro- or antiapoptotic signals. The values expressed as mean ± SEM (n = 5–10 in each group). **P < 0.01; ***P < 0.001; and ## P < 0.01.