Fig 2: Detection of transfected tPA gene in goat mammary epithelial cells by PCR. 1-8: monoclonal mammary epithelial cell DNA; M: DL2000 digested DNA Marker; N: negative control (normal cell genome); P: positive control (BLC14/tPA plasmid).

Fig 3: FAPA of goat mammary epithelial cell induction solution. 1: 1.0 µg/mL alteplase standard; 2: 100 µg/mL alteplase standard; 3: 90# tPA/GH double transgenic goat mammary epithelial cell induction liquid (diluted 100 times); 4: 108# tPA/GH double transgenic goat mammary epithelial cell induction liquid (diluted 1000 times); 5: 15# tPA single transgenic goat mammary epithelial cell induction fluid (diluted 10 times); 6: 32# tPA single transgenic goat mammary epithelial cell induction fluid (diluted 10 times); 7: normal nontransgenic goat mammary epithelial cell induction fluid; 8: PBS.

Fig 4: Gene structure of mammary gland-specific expression vectors BLC14/tPA and BLC14/GH.

Fig 5: Western blotting detection of tPA expression in transgenic monoclonal mammary epithelial cell lines. M: protein molecular weight standard; 1: positive control (tPA standard); 2: negative control (normal nontransgenic cell inducer); 3-6: expression of cell inducer (108#, 90#, 32#, and 15#).