Perilipin-2/ADFP Antibody

Product Specs
ItemPerilipin-2/ADFP Antibody - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberNB110-40877
ImmunogenA synthetic peptide made to a C-terminal region of mouse ADFP (within residues 350-425) [Swiss-Prot# P43883]
NCBI Gene AliasesADRP, ADPH, Adrp, ADFP, Adfp
Concentration1.0 mg/ml
Quantity0.1 ml (also 0.025 ml)
ApplicationsIF/IHC,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,SDS-Page,Simple Western,Dual RNAscope ISH-IHC,Immunocytochemistry/ Immunofluorescence
Conjugate/TagUnconjugated
FormatPBS
ReactivityBovine,Human,Mouse,Plant,Rat
TargetPerilipin-2/ADFP
HostRabbit
NCBI Full Gene Nameperilipin 2
ClonalityPolyclonal
PurityImmunogen affinity purified
Molecular Weight51 kDa
Research AreaCancer, Cardiovascular Biology, Diabetes Research, Epigenetics, Hypoxia, Lipid and Metabolism, Lipid Droplets, metabolism, Signal Transduction
IsotypeIgG
Antibody TypePrimary Antibodies
StorageAliquot and store at -20C or -80C. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Sizes Available: 0.1 ml (also 0.025 ml)

Questions and Answers

What species does this antibody work with, and has it been used in relevant studies?

The Perilipin-2/ADFP Antibody is reactive with multiple species, including mouse, bovine, human, plant, and rat. It has been utilized in various studies, such as in the research by Antony et al. (2022), where it was used to investigate the regulation of lipid and perilipin 2 levels in skeletal muscle.

Can this antibody be used for immunofluorescence, and what evidence supports its effectiveness in this application?

Yes, the Perilipin-2/ADFP Antibody has been shown to be effective for immunofluorescence applications. For instance, in the study by Ibayashi et al. (2024), the antibody was employed to visualize perilipin 2 in ovarian sections, demonstrating its utility in this technique.

Is there any evidence of this antibody being used in cancer research, particularly related to lipid metabolism?

Yes, the Perilipin-2/ADFP Antibody has been referenced in cancer research. For example, in the study by Taïb et al. (2019), it was used to examine lipid accumulation and oxidation in glioblastoma multiforme, highlighting its relevance in studies focused on lipid metabolism in cancer.

What are some specific applications where this antibody has been validated, and how has it been cited in the literature?

The Perilipin-2/ADFP Antibody has been validated for use in Western blotting, immunocytochemistry, immunohistochemistry, and in situ hybridization. It has been cited in at least 55 scientific articles, indicating its widespread use and acceptance in various research contexts, including studies on metabolic disorders and cancer.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Hepatic inflammation after exposure to a mixture of low-dose arsenic and cadmium in a murine model of fatty liver diseaseToxicological SciencesJuly 1, 2026Nivetha K Subramaniam, Natascha Gagnon, Cynthia Guilbert, Rushmi Perinpanathan, Madelyn Abraham, Christophe Goncalves, Jaymie R Meliker, Sonia Del Rincon, Koren K Mannblocking, sections were incubated with a primary antibody against perilipin-2 (Novus Biologicals, Cat No. NB110-40877, polyclonal antibody, 1:600). Detection was performed using an HRP-conjugated secondary antibody (Agilent

(2) A high‐fat, high‐sucrose diet exacerbates muscle and metabolic pathology and undermines glucocorticoid efficacy in dystrophin‐deficient miceJ PhysiolJuly 1, 2026Morgan E. Vorwald, Swathy Krishna, Ji Heun Lee, Melissa Roths, Rudy J. Valentine, Joshua T. SelsbyTBST,1:1000 TBST Parkin,Cell Signaling,2132,Rabbit,1:500 TBST,1:500 TBST Perilipin 2,Novus Biologicals,NB110‐40877,Rabbit,1:1000 TBST,1:1000 TBST Perilipin 5,Proteintech,26951‐1‐AP,Rabbit,1:1000 TBST,1:1000 TBST PINK1,Cell Signaling

(3) HDL-mimetic peptide treatment reverses APOE4-induced transcriptomic and lipidomic alterations in the brain of humanized APOE miceAlzheimers DementJune 23, 2026Allison Chang, Minwoo Kim, Matthew Glittenberg, Wenhui Qu, Danni Li, Ling Liblocking buffer. Primary antibodies included: rabbit anti‐perilipin‐2 (PLIN2; 1:500; Novus Biologicals, NB110‐40877); mouse anti‐MAP2 (1:500; Invitrogen, MA5‐12826); chicken anti‐glial fibrillary acidic protein (GFAP;

(4) Macrophage signaling and function are regulated by distinct sterol biochemistriesJournal of Lipid ResearchMay 7, 2026Jazmine D.W. Yaeger, Bijaya Pradhan, Jason G. Kerkvliet, Amelia G. Lawver, Sonali Sengupta, Natalie W. Thiex, Kevin R. Francis(Cell Signaling Technologies, 9367, 1:200), rat anti-CD68 (Bio-Rad, MCA1957, 1:500), and rabbit anti-perilipin-2 (Novus, NB110-40877, 1:200). After primary antibody incubation, cells were washed three times with PBS before being exposed

(5) Myeloid AEG-1/MTDH drives inflammation and hepatocellular dysfunction in diet-induced steatohepatitisJournal of Biological ChemistryFebruary 1, 2026Suchismita Raha, Saranya Chidambaranathan Reghupaty, Xufeng Qu, Rachel G. Mendoza, Devon Farrar, Ali Gawi Ermi, Eva Davis, Rebecca K. Martin, Mark A. Subler, Paul B. Fisher, et al.using primary antibodies, such as anti–PLIN2/adipophilin (rabbit; Novus Biologicals; catalog nos.: NB110-40877; 1:200 dilution); F4/80 antibody (rat; Bio-Rad; catalog no.: MCA497R; 1:100 dilution) and antineutrophil

(6) Lactate Dehydrogenase Inhibition Reverts the Fatty Acid‐Induced Neurotoxic Phenotype of AstrocytesGliaJanuary 6, 2026Daniel Esteve, Mariana Bresque, Daniel Okhuevbie, Sandhya Ramachandran, Mariana Pehar, Marcelo R. Vargasantibodies used were: GFAP Antibody (GA5) (Novus, #NBP2‐29415) and Perilipin‐2/ADFP antibody (Novus, #NB110‐40877). Secondary antibodies were as follows: Alexa Fluor 594 AffiniPure Donkey Anti‐Rabbit IgG (H + L) and

(7) Pioglitazone modulates metabolic adaptation and peripheral nerve regeneration after injuryMolecular neurodegenerationDecember 1, 2025Seitz A, Özöncü T, Sinske D, Klugmann C, Tews D, Fischer-Posovszky P, Knöll B, Meyer Zu Reckendorf S.antibodies: anti-PPARɣ (rabbit, 1:5000, Abcam, ab59256), anti-PLIN2/ADFP (rabbit, 1:500, Novus biologicals, NB110-40877), anti-CD45 (rat, 1:100, BD Pharmingen, 550539), and anti-cJUN (rabbit, 1:500, Cell Signaling, #9165).

(8) Knockout of Perilipin-2 in Microglia Alters Lipid Droplet Accumulation and Response to Alzheimer’s Disease StimuliCellsNovember 13, 2025Isaiah O. Stephens, Lance A. Johnsonanti-Iba1 (Novus NB100-1028, 1:500) and rabbit anti-PLIN2/ADRP (Novus Biologicals, Centennial, CO, USA, NB110-40877, 1:500). After three 10-min PBS-T washes, sections were incubated 2 h at room temperature (protected

(9) Oral administration of the probiotic strain Lactobacillus helveticus BGRA43 reduces high-fat diet–induced hepatic steatosis in mice and restores gut microbiota compositionFrontiers in PharmacologyNovember 4, 2025Ana Teofilović, Miloš Vratarić, Biljana Bursać, Ljupka Gligorovska, Danijela Vojnović Milutinović, Nemanja Stanisavljević, Ivana Strahinić, Danijela Mišić, Filip Nikolić, Bojan Pavlović, et al.primary antibodies: anti-SREBP-1c (1:500, sc-366, Santa Cruz Biotechnology), anti-perilipin 2 (1:1000, NB110-40877, Novus Biologicals), anti-NOD-like receptor protein 3 (NLRP3) (1:500, NBP2-12446, Novus Biologicals),

(10) Gut microbiota composition combined with reduced intestinal fatty acid uptake prevents hepatic steatosis in obesity-resistant mice fed a high-fat dietScientific reportsOctober 23, 2025Vratarić M, Teofilović A, Vojnović Milutinović D, Veličković N, Đmura G, Djordjevic A.ab54481, Abcam), anti-CPT1 (1:500, sc-139482, Santa Cruz Biotechnology), anti-perilipin-2 (1:1000, NB110-40877, Novus Biologicals), anti-GLUT2 (1:1000, ab192599, Abcam), anti-IRS1 (1:500, FNab04400, FineTest), anti-IRS2

(11) One day of environment‐induced heat stress causes injury to the murine kidneyExp PhysiolOctober 2, 2025Melissa Roths, Alyona Michael, Zachary J. Schlader, Joshua T. Selsbymouse,1:1000,1:1000 Perilipin (PLIN) 1,"Novus, NB100‐60554 goat",1:1000,1:2000 Perilipin (PLIN) 2,"Novus, NB110‐40877",1:1000,1:1000 Peroxisome proliferator‐activated receptor alpha (PPARα),"Proteintech, 66826‐1 mouse",1:1000,1:1000 Peroxisome

(12) Macrophage signaling and function are regulated by distinct sterol biochemistriesbioRxivAugust 11, 2025Jazmine D. W. Yaeger, Jason G. Kerkvliet, Bijaya Pradhan, Amelia G. Lawver, Sonali Sengupta, Natalie W. Thiex, Kevin R. Francis(Cell Signaling Technologies, 9367, 1:200), rat anti-CD68 (Bio-Rad, MCA1957, 1:500), and rabbit anti-perilipin-2 (Novus, NB110-40877, 1:200). After primary antibody incubation, cells were washed three times with PBS before being exposed

(13) Protein carbamylation in atherosclerotic plaques correlates with uremia and disease progression, localizing predominantly to foam cellsFrontiers in ImmunologyMay 28, 2025Valeria Saar-Kovrov, Aleksandra Pawlowska, Adrien Guillot, Marion J. J. Gijbels, Judith C. Sluimer, Lieve Temmerman, Pieter Goossens, Barend M. E. Mees, Frank Tacke, Vera Jankowski, et al.above using mouse-anti-human CD68 antibody (1:100; M0814; Agilent) and rabbit-anti-human PLIN2 (1:50; NB110-40877; Novus Biologicals). The secondary antibodies used were goat-anti-mouse (1:500; Alexa Fluor 647; 4410S;

(14) DHCR7 inhibition ameliorates MetALD and HCC in mice and human 3D liver spheroidsJHEP ReportsApril 5, 2025Gen Yamamoto, Raquel Carvalho-Gontijo Weber, Wonseok Lee, Vivian Zhang, Haeum Jang, Sadatsugu Sakane, Xiao Liu, Hyun Young Kim, David A. Brenner, Na Li, et al.type I (72026S, Cell Signaling Technology, 1:1,000), CYP2E1 (AB1252, Millipore, 1:1,000), Perilipin 2 (NB110-40877, Novus Biologicals, 1:1,000), PAI-1 (13801-1-AP, Proteintech, 1:1,000), ɑSMA (ab5694, Abcam, 1:2,000),

(15) Loss of fatty acid degradation by astrocytic mitochondria triggers neuroinflammation and neurodegenerationNature MetabolismMarch 23, 2023Yashi Mi, Guoyuan Qi, Francesca Vitali, Yuan Shang, Adam C. Raikes, Tian Wang, Yan Jin, Roberta D. Brinton, Haiwei Gu, Fei YinNF-κB antibody (Cell Signaling Technology, 8242, 1:1000), perilipin-2/ADFP antibody (Novus Biologicals, NB110–40877, 1:200), PFKFB3 (D7H4Q) antibody (Cell Signaling Technology, 13123s, 1:1000), Phospho-AMPKα (Thr172)

(16) Characterization of a second sterol-esterifying enzyme in Toxoplasma highlights the importance of cholesterol storage pathways for the parasiteMolecular microbiologyJanuary 1, 0001"Lige B, Sampels V, Coppens I."

(17) Lipolytic signaling in response to acute exercise is altered in female mice following ovariectomyJournal of cellular biochemistryJanuary 1, 0001"Wohlers LM, Jackson KC, Spangenburg EE."

(18) C/EBP homologous protein (CHOP) contributes to suppression of metabolic genes during endoplasmic reticulum stress in the liverThe Journal of biological chemistryJanuary 1, 0001"Chikka MR, McCabe DD, Tyra HM, Rutkowski DT."

(19) Perilipin 2 (PLIN2)-deficiency does not increase cholesterol-induced toxicity in macrophagesPloS oneJanuary 1, 0001"Son SH, Goo YH, Chang BH, Paul A."

(20) Resveratrol increases CD68⁺ Kupffer cells colocalized with adipose differentiation-related protein and ameliorates high-fat-diet-induced fatty liver in miceMolecular nutrition & food researchJanuary 1, 0001"Nishikawa K, Iwaya K, Kinoshita M, Fujiwara Y, Akao M, Sonoda M, Thiruppathi S, Suzuki T, Hiroi S, Seki S, et al."

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