Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [Rhodamine] (Pre-adsorbed) [NB120-7087] - Goat anti-Rabbit IgG (H+L) Secondary Antibody [Rhodamine] [NB120-7087] - Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane 4: Rabbit IgM Whole Molecule. Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for Goat anti-Rabbit IgG (H+L) Secondary Antibody [Rhodamine] (Pre-adsorbed)
Available conjugates: Available conjugates: DyLight 800, PE/Cy7, Rhodamine, DyLight 550Specificity: This antibody was pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.Sizes Available: 0.5 mg