Fig 1: Labeling of nicotinic acetylcholine receptors (nAChRs) and presynaptic proteins in diaphragm NMJs of ΔFUS(1-359) mice vs. those of WT mice. (A–F) Comparison between ΔFUS(1-359) mice at pre-onset (A–C) and onset (D–F) stages and age-matched WT animals. (A,D) Representative fluorescent and overlay images. Scale bars—20 µm. (B,E) Fluorescence intensity of tetramethylrhodamine-conjugated αBtx and calculation of number of end-plate fragments. (C,F) Immunoexpression of synapsin 1 and SNAP-25. * p ˂ 0.05, ** p ˂ 0.01 according to Mann–Whitney U test compared to that for WT mice. n = 5–6 muscles from individual mice per group; 11–15 NMJs from each muscle. Arrows indicate an increase or decrease in parameter. NS—non significant.
Fig 2: Immunohistochemical analysis of GPRC6A in taste cells of mouse circumvallate papilla taste buds. (A,B) Fluorescence micrographs of the taste buds immunostained with the anti-GPRC6A antibody in the absence (A) or presence (B) of the antigen peptide (left panels; middle panels, Nomarski images of the left panels; right panels, merged images of respective right and middle panels). (C) Immunoreactivity for GPRC6A is expressed in a small subset of type II taste cells. Shown are immunoreactivity for GPRC6A (red, left panel), immunoreactivity for a-gustducin (green, middle panel) and a merged image of both panels (right panel). Arrowheads indicate GPRC6A-positive type II taste cells. (D) Immunoreactivity for GPRC6A is expressed in a subset of type III taste cells. Shown are immunoreactivity for GPRC6A (red, left panel), immunoreactivity for SNAP-25 (green, middle panel) and a merged image of both panels (right). Arrowheads indicate GPRC6A-positive type III taste cells. Scale bars, 20 mm.
Fig 3: Immunohistochemical analysis of colocalization of GPRC6A and cell type-specific markers in taste cells of rat fungiform papilla taste buds.(A) Some but not all GPRC6A-immunopositive cells exhibited immunoreactivity for IP3R3, a marker of most type II cells. White arrow indicates GPRC6A/IP3R3 double-positive taste cell. (B) α-Gustducin, another marker of a subset of type II cells, was unlikely to colocalize with GPRC6A in single taste cells. (C, D) Neither 5-HT (C) nor SNAP25 (D), specific markers of type III taste cells, were likely to colocalize with GPRC6A in single taste cells. Scale bars, 10 µm. GPRC6A, G-protein-coupled receptor family C group 6 subtype A; IP3R3, inositol 1,4,5-trisphosphate receptor type 3; 5-HT, 5-hydroxytryptamine; SNAP25, synaptosomal-associated protein 25 kDa.
Fig 4: Immunohistochemical analysis of colocalization of GPRC6A and cell type-specific markers in taste cells of rat fungiform papilla taste buds.(A) Some but not all GPRC6A-immunopositive cells exhibited immunoreactivity for IP3R3, a marker of most type II cells. White arrow indicates GPRC6A/IP3R3 double-positive taste cell. (B) α-Gustducin, another marker of a subset of type II cells, was unlikely to colocalize with GPRC6A in single taste cells. (C, D) Neither 5-HT (C) nor SNAP25 (D), specific markers of type III taste cells, were likely to colocalize with GPRC6A in single taste cells. Scale bars, 10 µm. GPRC6A, G-protein-coupled receptor family C group 6 subtype A; IP3R3, inositol 1,4,5-trisphosphate receptor type 3; 5-HT, 5-hydroxytryptamine; SNAP25, synaptosomal-associated protein 25 kDa.
Supplier Page from Abcam for Anti-SNAP25 antibody