Western blot was performed using anti-Flotillin 2 Polyclonal Antibody (Product # PA5-30033) and a 53 kDa band corresponding to FLOT2 was observed across cell lines tested and increased upon Cisplatin treatment in SW480, along with an uncharacterized band (*) at ~60 kDa in the cell lines tested. Whole cell extracts (30 µg lysate) of SW480 (Lane 1), SW480 treated with Cisplatin (10µM for 24 hours) (Lane 2), Molt-4 (Lane 3) and HeLa (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).