Cyclin B1 (D5C10) XP ® Rabbit mAb 12231S from Cell Signaling Technology

Product Specs
ItemCyclin B1 (D5C10) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number12231S
Quantity100 µl
ApplicationsW, W-S, IP, IF-IC, FC-FP
ReactivityH, R
ClonalityMonoclonal
HostRabbit
TargetCyclin B1
NCBI Full Gene Namecyclin B1
NCBI Gene AliasesCCNB
Research AreaCell Cycle / Checkpoint Control
IsotypeIgG
CloneD5C10
Molecular Weight55

Questions and Answers

Can this antibody be used to study the role of Cyclin B1 in mitosis?

Yes, the Cyclin B1 (D5C10) XP® Rabbit mAb has been shown to be effective in various applications, including Western blotting and immunofluorescence, to study Cyclin B1's role in cell cycle regulation during mitosis. For instance, it has been utilized to investigate the phosphorylation of DENR by Cyclin B/CDK1, which is crucial for mitotic protein translation and cell division fidelity ().

Is this antibody suitable for use in flow cytometry?

Yes, the Cyclin B1 antibody has been demonstrated to work in flow cytometry applications. It has been cited in studies that analyze cell cycle progression and checkpoint control, making it a valuable tool for researchers examining these processes in various cell types ().

What species can this antibody detect?

The Cyclin B1 (D5C10) XP® Rabbit mAb is reactive with human and rat samples, making it suitable for studies involving these species. Its specificity for Cyclin B1 allows for the investigation of cell cycle dynamics in both human and rat cell lines ().

Are there any known applications of this antibody in cancer research?

Yes, the Cyclin B1 antibody has been used in cancer research to explore mechanisms of drug resistance and cell cycle dysregulation. For example, it has been implicated in studies examining how aberrant signaling pathways, such as those involving the androgen receptor, can lead to mitotic checkpoint dysregulation and resistance to chemotherapy in prostate cancer ().

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Nebivolol suppresses glioblastoma progression via dual modulation of mitochondrial metabolism and AKT/mTOR/4EBP1 signaling axisPLoS GenetApril 24, 2026Lingni Zhou, Hongyu Che, Hongyan Jiang, Luxin Yin, Yongang Jiang, Yuhui Zhang, Haoran Liang, Rutong Yu, Xu Zhang, Xuejiao Liumedium before use. Bax (#2772), Bcl-xl (#2762), p21 (#2947), p27 (#3686), cyclin D1 (#2922), cyclin B1 (#12231), CDK6 (#3136), AKT (#9272), p-AKT (#4058), p-mTOR (#5536), mTOR (#2983), p-4EBP1 (#2855), 4EBP1 (#9644),

(2) QW-5-70 targets the colchicine site and demonstrates antitumor activity in P-gp-overexpressing cancer modelsMol Cancer TherApril 22, 2026Yang Xie, Ruida Hou, Najah Albadari, Hao Chen, Darcie J. Miller, Judith Quadrozzi Quadrozzi Gruntz, Michael L. Oldham, Mir Shahriar Kamal, Jianxiong Jiang, Zhongzhi Wu, et al.(CST, #9664, RRID: AB_2070042), cleaved-caspase9 (CST, #7237, RRID: AB_10895832), Cyclin B1 (CST, #12231, RRID: AB_2783553), phospho-CDK1 (Thr161) (CST, #9114, RRID: AB_2074652), phospho-Histone H3 (CST, #9701,

(3) Denaturing SUMO Immunoprecipitation From Mitotic CellsBIO-PROTOCOLApril 5, 2026Alexandra K. Walker, Alexander J. Lanz, Joanna R. Morris(ThermoFisher Scientific, catalog number: 22663) 41. (Optional) Cyclin B1 antibody (Cell Signaling, catalog number: 12231) 42. (Optional) α Tubulin antibody (Novus Biologicals, catalog number: NB100-690) 43. (Optional) Histone H3

(4) FBXW7 Targets the SPT6-ΔNp63 Axis for Degradation to Inhibit Esophageal Tumorigenesis Induced by 4-Nitroquinoline N-OxideMedComm (2020)March 4, 2026Jiagui Zhang, Lu Yin, Xiahong You, Xiufang Xiong, Yi Sun(ABclonal, AE012), cleaved‐NOTCH1 (Cell Signaling Technology, 4147), Cyclin B1 (Cell Signaling Technology, 12231P), PLK1 (Proteintech, 10305‐1‐AP), PARP (Cell Signaling Technology, 9542S), Cleaved PARP (Cell Signaling Technology

(5) Macrophage-delivered Nifuroxazide reprograms immunosuppressive microenvironment and synergizes with oxaliplatin for enhanced anti-hepatocellular carcinoma therapyJournal of NanobiotechnologyFebruary 23, 2026Zhentao Yang, Hong Tang, Ning Wang, Ruiqi Sun, Ke Zhou, Wentao Zhao, Zhi Liang, Haojing Ma, Haoyu Li, Penghong Song, et al.Phospho-β-CateninSer552, (Cat# 5651, CST, USA), c-Myc (Cat# 18583, CST, USA), c-Jun (Cat# 9165, CST, USA), Cyclin-B1 (Cat# 12231, CST, USA), Cdc2 (Cat# 9116, CST, USA), Cleaved PARP (Cat# 5625, CST, USA), Cleaved Caspase-3 (Cat#

(6) FAM64A Potentiates Bladder Carcinoma Tumorigenesis and Metastasis Through PI3K/mTORC2/AKT Pathway ActivationCancers (Basel)February 6, 2026Tao Zhu, Cen Liufu, Cong Yin, Jinqing He, Junhua Luo, Bentao Shi, Yan WangFAM64A (Thermo Fisher H00054478-D01P, 1:1000), β-tubulin (Proteintech 2013, 1:10,000), Cyclin B1 (CST 12231, 1:1000), Ki-67 (Proteintech 241499E7, 1:10,000), AKT (CST 11E7, 1:1000), mTOR (CST 2983, 1:1000), PI3K

(7) IGFBP-6 regulates breast cancer cell cycle progression by promoting exit out of G1Journal of Biological ChemistryFebruary 1, 2026Francisco J. Lariz, Shayla Hernandez, Diana C. Bautista-Tovar, Kevin D. HoustonCell Signaling), Phospho-STAT2 (Y690) (#88410 Cell Signaling), STAT2 (#72604 Cell Signaling), Cyclin B1 (#12231 Cell Signaling), Aurora A (#14475 Cell Signaling), CDC20 (#14866 Cell Signaling), HMMR (#55463 Cell Signaling

(8) Multi-omic analysis of human PHACTR1 signaling networksCommunications BiologyJanuary 19, 2026Kathryn Wolhuter, Lijiang Ma, Nicole S. Bryce, Osvaldo Contreras, Natalie Mellett, Ling Zhong, Chris Thekkedam, Siiri E. Iismaa, Corey Giles, Richard P. Harvey, et al.(#8064), Thymidine Kinase 1 (#28755), Phospho-Histone H3 (Ser10, #53348), Cyclin A2 (#91500), Cyclin B1 (#12231), Cyclin E1 (#20808), Phospho-cdc2 (Tyr15, #4539), Tom20 (#42406), OPA1 (#80471), DRP1 (#8570), Phospho-DRP1

(9) HMMR/RHAMM recruits SACK1D/FAM83D-CK1α complex at the mitotic spindle to control spindle alignmentiScienceJanuary 16, 2026Tyrell N. Cartwright, Naveen K. Nakarakanti, Karen Dunbar, Luke J. Fulcher, Selina Bader, Nicola T. Wood, Thomas J. Macartney, Gopal P. SapkotaPericentrin,Invitrogen,"Thermo Fisher Scientific Cat# PA5-54109, RRID:AB_2645392" Rabbit Cyclin B1,CST,12231S Rabbit GAPDH (HRP conjugated),ProteinTech,"Proteintech Cat# HRP-60004, RRID:AB_2737588" "Goat anti-Rabbit

(10) Identification of hub genes and construction of a survival prediction model for patients with nasopharyngeal carcinomaScientific ReportsJanuary 16, 2026Jinfei Zhu, Yizhuo Feng, Zhanwei Zhu, Yubin Chen, Can-e TangCell Signaling Technology), anti-CCNA2 (1:200 dilution; #67955; Cell Signaling Technology), and anti-CCNB2 (1:200 dilution; #12231; Cell Signaling Technology). Construction and evaluation of prediction model Age and gender were included

(11) Apigenin Alleviates Zearalenone-Induced Oxidative Stress and Apoptosis in Swine Testis Cells Through the Wnt Signaling PathwayAntioxidants (Basel)December 29, 2025Chenyun Guo, Yidan Zhang, Yiwei Wang, Yile Sun, Haoze Ning, Jiaxin Gao, Fei Guo, Pengyun Ji, Lu Zhang, Guoshi Liu, et al.Antibody Name,Catalog No.,Vendor,Dilution Factor PCNA,2586S,Cell Signaling Technology,1:2000 CCNB1,12231S,Cell Signaling Technology,1:1000 CCNA2,4656S,Cell Signaling Technology,1:2000 Bcl-2,F0125,Selleck,1:1000 Caspase3,14220S,Cell Signaling Technology

(12) Neutrophils Extracellular Traps Impair Lung Endothelial Proliferation in Sepsis via PLK1 Inhibition and Cell Cycle ArrestJ Inflamm ResDecember 27, 2025Chenyu Zhu, Mengdi Qu, Dan Wu, Yuxin Shi, Fu Zeng, Changhong Miao, Di Zhouspecific primary antibodies: anti-PLK1 (1:500, 4513, Cell Signaling Technology), anti-CyclinB1 (1:1000, 12231, Cell Signaling Technology), anti-p-CDC25C (1:250, 9529, Cell Signaling Technology), anti-CDC25C (1:1000,

(13) Sappanchalcone suppresses NSCLC by oxidative stress-driven DNA damage and ER stress activation through PIEZO1 modulationiScienceDecember 19, 2025Weiyu Wu, Ren Zhang, Geer Chen, Ziyu Chen, Zicong Lin, Yin Chen, Jiaqi Li, Weilin Liao, Junyi Wang, Xiaoxuan Wang, et al.anti-Phospho-CDC2 (Tyr15),Cell Signaling Technology,Cat#4539; RRID: AB_560953 anti-Cyclin B1,Cell Signaling Technology,Cat#12231; RRID: AB_2783553 anti-KEAP1,Cell Signaling Technology,Cat#7705; RRID: AB_10860422 β-ACTIN,Cell Signaling Technology

(14) SBFI Inhibitors Reprogram Transcriptomic Landscape of Prostate Cancer Cells Leading to Cell DeathCancers (Basel)November 21, 2025Shubhra Rajput, Joseph F. LaComb, Chris Gordon, Hehe Wang, Manisha Sarder, Martin Kaczocha, Iwao Ojima, Agnieszka B. Bialkowska(Cell signaling Technology, 67955, RRID:AB_2909603 1:1000 in BSA), anti-Cyclin B1 (Cell Signaling Technology, 12231, RRID:AB_2783553, 1:1000 in BSA), anti-Cyclin D1 (BioCare Medical, Pacheco, CA, USA, CRM307A, 1:1000

(15) Mutant p53 protein accumulation is selectively targetable by proximity-inducing drugsNature Chemical BiologyNovember 3, 2025Ananthan Sadagopan, Maximilian Carson, Eriks J. Zamurs, Nicholas Garaffo, Heng-Jui Chang, Stuart L. Schreiber, Matthew Meyerson, William J. Gibsona PVDF membrane and treated with primary antibodies: cyclin B1 (D5C10, Cell Signaling Technologies, 12231T; 1:1,000) and phospho-PLK-binding motif (D73F6, Cell Signaling Technologies, 5243T; 1:1,000). The membrane

(16) BATF2-mediated control of astrocyte proliferationJournal of Biological ChemistryNovember 1, 2025Rachel A. Tinkey, Benjamin J. Frostino, Maria L. Habean, Jessica L. Williamsand Proteintech, 60186-I-Ig), CDK2 (78B2) (Cell Signaling, 2546S), cyclin B1 (D5C10) (Cell Signaling, 12231S), and vinculin (Invitrogen, 700062). The following day, membranes were washed with TBST three times

(17) Oxphos targeting of MYCN-amplified neuroblastomaFrontiers in OncologyOctober 24, 2025Soraya Epp, Donagh Egan, Evon Poon, Amirah Adlina Abdul Aziz, Kieran Wynne, Louis Chesler, Melinda Halasz, Walter Kolch#3465, Cell Signaling Technology; Phospho-Histone H2AX (Ser139) #05-636, Sigma-Aldrich; Cyclin B1 #12231, Cell Signaling Technology; Cyclin D1 #2922, Cell Signaling Technology; GAPDH #2118, Cell Signaling Technology

(18) Reversible arginine methylation of PI3KC2α controls mitotic spindle dynamicsCell Commun SignalOctober 2, 2025Yena Cho, Jee Won Hwang, Mark T. Bedford, Dong Hee Na, Dae-Geun Song, Su-Nam Kim, Yong Kee KimSanta Cruz Biotechnology, Dallas, TX, USA), CARM1 (A300-421 A; Bethyl Laboratories, Montgomery, TX, USA), Cyclin B1 (#12231; Cell Signaling Technology, Danvers, MA, USA), Cyclin D3 (#2936; Cell Signaling Technology), EB1 (#2164;

(19) TUFT1 regulates cancer progression by suppressing centrosome amplification and mitotic spindle multipolarityCell Death & DiseaseSeptember 29, 2025Shaojie Feng, Mengmeng Zhao, Donghui Zhang, Yan Zhang, Lingyuan Min, Xianqiang Liu, Huan Shi, Tianning Wanganti-Cep164 (22227-1-AP, Proteintech), rabbit anti-Odf2 (12058-1-AP, Proteintech), rabbit anti-Cyclin B1 (12231, Cell Signaling Technology), and mouse anti-NEK2 (610593, BD Biosciences, NJ, USA). Secondary antibodies

(20) Dual molecular therapy targeting tumor cell heterogeneity improves therapeutic efficacy in glioblastomaiScienceSeptember 19, 2025Shuichiro Hirano, Atsuhito Uneda, Yoshihiro Otani, Yasuki Suruga, Ryoji Imoto, Madoka Hokama, Tsuyoshi Umeda, Ryosuke Ikemachi, Shohei Nishigaki, Nobushige Tsuboi, et al.Cell Signaling Technology, Danvers, MA, US, #4539), anti-Cyclin B1 (1:1000, Cell Signaling Technology, #12231), anti-phospho-Akt (Ser 473) (1:1000, Cell Signaling Technology, #4060), anti-Akt (1:1000, Cell Signaling Technology

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Cyclin B1 (D5C10) XP ® Rabbit mAb

Cyclin B1 (D5C10) XP ® Rabbit mAb from Cell Signaling Technology

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Questions and Answers

Cell Signaling Technology

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Cyclin B1 (D5C10) XP ® Rabbit mAb from Cell Signaling Technology

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