Fig 1: Laminin activated integrin a6ß4 signals to promote tumor development. A, the expression of ITGA3, ITGA6, ITGA7, ITGB1 and ITGB4 in Biu-87 cells cultured on dish and 2D laminin/collagen gels. B, western blotting of integrin a6 and ß4 in Biu-87 and MB49 cells cultured on dish and 2D laminin/collagen gels. C, the relative expression of ITGA6 and ITGB4 in Biu-87 cells treated with scrambled siRNA, ITGA6 siRNA and ITGB4 siRNA. D, cell proliferation of 2D laminin/collagen cultured Biu-87 cells treated with scrambled siRNA, ITGA6 siRNA and ITGB4 siRNA. E, relative migrating cells of 2D laminin/collagen cultured Biu-87 cells treated with scrambled siRNA, ITGA6 siRNA and ITGB4 siRNA. F, cell proliferation of dish cultured Biu-87 cells treated with scrambled siRNA, ITGA6 siRNA and ITGB4 siRNA. G, relative migrating cells of dish cultured Biu-87 cells treated with scrambled siRNA, ITGA6 siRNA and ITGB4 siRNA. H, immunofluorescence of integrin a6 in tumor tissues from NMIBC and MIBC patients. The expression of integrin a6 was quantified in each group (n = 15). The scale bar was 25 µm. I, immunofluorescence of integrin ß4 in tumor tissues from NMIBC and MIBC patients. The expression of integrin ß4 was quantified in each group (n = 15). The scale bar was 25 µm