Myosin IIb (D8H8) XP ® Rabbit mAb 8824S from Cell Signaling Technology

Product Specs
ItemMyosin IIb (D8H8) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number8824S
Quantity100 µl
ApplicationsW, IF-IC
ReactivityH, M, R, Mk
ClonalityMonoclonal
TargetMyosin IIb
HostRabbit
NCBI Gene AliasesNMMHC-IIB, NMMHCB
NCBI Full Gene Namemyosin heavy chain 10
Research AreaCytoskeletal Signaling
IsotypeIgG
CloneD8H8
Molecular Weight230

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Epithelial cell chirality emerges through the dynamic concentric pattern of actomyosin cytoskeletonElifeJuly 8, 2025Takaki Yamamoto, Tomoki Ishibashi, Yuko Mimori-Kiyosue, Sylvain Hiver, Naoko Tokushige, Mitsusuke Tarama, Masatoshi Takeichi, Tatsuo Shibataanti-Myosin IIA (Sigma-Aldrich, M8064, dilution 1:1000); rabbit anti-Myosin IIB (Cell Signaling Technology, 8824, dilution 1:1000); mouse anti-GAPDH (Santa Cruz Biotechnology, sc-166574, dilution 1:1000); HRP-conjugated

(2) Epithelial cell chirality emerges through the dynamic concentric pattern of actomyosin cytoskeletonElifeJuly 8, 2025Takaki Yamamoto, Tomoki Ishibashi, Yuko Mimori-Kiyosue, Sylvain Hiver, Naoko Tokushige, Mitsusuke Tarama, Masatoshi Takeichi, Tatsuo Shibataanti-Myosin IIA (Sigma-Aldrich, M8064, dilution 1:1000); rabbit anti-Myosin IIB (Cell Signaling Technology, 8824, dilution 1:1000); mouse anti-GAPDH (Santa Cruz Biotechnology, sc-166574, dilution 1:1000); HRP-conjugated

(3) Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systemsCell reportsJune 24, 2025Tidball AM, Luo J, Walker JC, Yang CY, Lee K, Spencer RC, Matthews C, Feng G, Hsu PP, Lee Y, et al.(1:1000),Sigma,Cat#G8795; RRID: AB_1078991 Rabbit monoclonal anti-MyoIIB (1:1000),Cell Signaling,Cat#8824; RRID: AB_11217639 Rabbit polyclonal anti-Myosin IIB (1:1000),Biolegend,Cat#909901; RRID: AB_2565101 Mouse

(4) Physiological role and mechanisms of action for a long noncoding haplotype regionCell ReportsJune 24, 2025Hong Xue, Manoj K. Mishra, Yong Liu, Pengyuan Liu, Michael Grzybowski, Rajan Pandey, Kristie Usa, Mark A. Vanden Avond, Niharika Bala, Abdel A. Alli, et al.vascular smooth muscle cells, anti-human α-SMA (1:200, sc-130616, Santa Cruz Biotechnology) or Myosin IIb (1:200, 8824S, Cell Signaling Technology) primary antibodies were used. After washing three times with PBS-T (PBS

(5) Protein covariation networks for elucidating ferroptosis inducer mechanisms and potential synergistic drug targetsCommunications BiologyMarch 31, 2025Rina Kunishige, Yoshiyuki Noguchi, Naomi Okamoto, Lei Li, Akito Ono, Masayuki Murata, Fumi Kano(Invitrogen; #PA5-100033; 1:400), F-actin (Phalloidin) (Invitrogen; #A22283; 1:200), Myosin IIB (CST; #8824; 1:200), Beta tubulin (SIGMA; #T8328; 1:500), pFAK (abcam; #ab81298; 1:400), FAK (Invitrogen; #39-6500;

(6) MRCK activates mouse oocyte myosin II for spindle rotation and male pronucleus centrationJournal of Cell BiologyNovember 6, 2023Anne Bourdais, Benoit Dehapiot, Guillaume HaletCell Signaling Technology), non-muscle myosin IIA (1:100; ab24762; Abcam), myosin IIB (D8H8; 1:100; #8824; Cell Signaling Technology), MRCKβ (A-2; 1:100; sc-390127; Santa Cruz Biotechnology), tubulin (1:200;

(7) Cell contractility drives mechanical memory of oral squamous cell carcinomaMolecular Biology of the CellAugust 1, 2023So Youn Moon, Paloma Santos de Campos, Bibiana Franzen Matte, Jesse K. Placone, Virgı´lio G. Zanella, Manoela D. Martins, Marcelo Lazzaron Lamers, Adam J. Englerantibody at 4°C for 24 h to detect NMM2A (1:500, Cell Signaling, #3403), NMM2B (1:500, Cell Signaling, #8824), NMM2C (1:500, Cell Signaling, #8189), E-cadherin (1:1000, Cell Signaling, #3915), N-cadherin (1:1000,

(8) Cell contractility drives mechanical memory of oral squamous cell carcinomaMol Biol CellAugust 1, 2023So Youn Moon, Paloma Santos de Campos, Bibiana Franzen Matte, Jesse K. Placone, Virgı´lio G. Zanella, Manoela D. Martins, Marcelo Lazzaron Lamers, Adam J. Englerantibody at 4°C for 24 h to detect NMM2A (1:500, Cell Signaling, #3403), NMM2B (1:500, Cell Signaling, #8824), NMM2C (1:500, Cell Signaling, #8189), E-cadherin (1:1000, Cell Signaling, #3915), N-cadherin (1:1000,

(9) Down‐regulation of MYH10 driven by chromosome 17p13.1 deletion promotes hepatocellular carcinoma metastasis through activation of the EGFR pathwayJournal of Cellular and Molecular MedicineDecember 1, 2021Qian Jin, Min Cheng, Xia Xia, Yuqing Han, Jing Zhang, Pengbo Cao, Gangqiao Zhoumouse monoclonal anti‐Flag (1:1000; NBP1‐06712, MBL, Japan), rabbit monoclonal anti‐MYH10 (1:1000; 8824, CST, USA), rabbit monoclonal anti‐EGFR (1:1000; 4267, CST, USA), rabbit monoclonal anti‐phosphorylated‐EGFR

(10) EFhd2 brain interactome reveals its association with different cellular and molecular processesJournal of neurochemistryDecember 1, 2021Soliman AS, Umstead A, Grabinski T, Kanaan NM, Lee A, Ryan J, Lamp J, Vega IE.Rabbit polyclonal anti-myosin 2a (cat. no. 3403, 1:500), Rabbit monoclonal anti-myosin 2b (cat. no. 8824, 1:500). 2.3 | Monoclonal Anti-EFhd2 antibody production A mouse monoclonal anti-EFhd2 (clone 10D6)

(11) Membrane-bound SCF and VCAM-1 synergistically regulate the morphology of hematopoietic stem cellsJournal of Cell BiologyOctober 4, 2021Jia Hao, Hao Zhou, Kristen Nemes, Daniel Yen, Winfield Zhao, Charles Bramlett, Bowen Wang, Rong Lu, Keyue Shenmyosin IIa antibody (3C7; catalog no. H00004627-M01; NovusBio) and myosin IIb antibody (D8H8; catalog no. 8824; Cell Signaling). Cell nucleus was visualized with DAPI staining. The Alexa Fluor 405–, 488–, or 568–conjugated

(12) Precise Tuning of Cortical Contractility Regulates Cell Shape during CytokinesisCell reportsApril 7, 2020Taneja N, Bersi MR, Baillargeon SM, Fenix AM, Cooper JA, Ohi R, Gama V, Merryman WD, Burnette DT.Rabbit anti-myosin IIA (#909801) was purchased from BioLegend (San Diego, CA). Rabbit anti-myosin IIB (#8824S and #3404S) was purchased from Cell Signaling Technology (Danvers, MA). The #8824S antibody was used

(13) Myosin IIA drives membrane bleb retractionMolecular biology of the cellApril 15, 2019Taneja N, Burnette DT.Rabbit anti-myosin IIA (#909801) was purchased from BioLegends (San Diego, CA). Rabbit anti-myosin IIB (#8824S) and myosin IIC (#8189) were purchased from Cell Signaling Technology (Danvers, MA). Mouse anti-tubulin

(14) BVES is required for maintenance of colonic epithelial integrity in experimental colitis by modifying intestinal permeabilityMucosal immunologySeptember 1, 2018Choksi YA, Reddy VK, Singh K, Barrett CW, Short SP, Parang B, Keating CE, Thompson JJ, Verriere TG, Brown RE, et al.Signaling, 3674S), and Myosin IIc (1:200, Cell Signaling, 8189S), Myosin IIb (1:200, Cell Signaling, 8824T), and Phalloidin (1:500, Biotium, 00043T). Secondary antibodies were conjugated to either 488 or 594

(15) Manic Fringe deficiency imposes Jagged1 addiction to intestinal tumor cellsNature communicationsJuly 31, 2018López-Arribillaga E, Rodilla V, Colomer C, Vert A, Shelton A, Cheng JH, Yan B, Gonzalez-Perez A, Junttila MR, Iglesias M, et al.

(16) Reducing insulin via conditional partial gene ablation in adults reverses diet-induced weight gainFASEB journal : official publication of the Federation of American Societies for Experimental BiologyMarch 1, 2018Page MM, Skovsø S, Cen H, Chiu AP, Dionne DA, Hutchinson DF, Lim GE, Szabat M, Flibotte S, Sinha S, et al.

(17) BioID identifies proteins involved in the cell biology of caveolaePloS oneJanuary 1, 2018Mendoza-Topaz C, Yeow I, Riento K, Nichols BJ.

(18) MiR-27b Promotes Muscle Development by Inhibiting MDFI ExpressionCellular Physiology and BiochemistryJanuary 1, 2018Lianjie Hou, Jian Xu, Yiren Jiao, Huaqin Li, Zhicheng Pan, Junli Duan, Ting Gu, Chingyuan Hu, Chong WangfluMyoD Monoclonal abcam ab16148 1:2000 oride. Electrophoresis supplies Myogenin Monoclonal abcam ab1835 1:2000 Myosin Monoclonal CST #8824 1:3000 were purchased from Bio-Rad β-Actin Monoclonal Bioss bsm-33036M 1:1000 (California, USA). The

(19) Injury induced expression of caveolar proteins in human kidney tubules - role of megakaryoblastic leukemia 1BMC nephrologyOctober 24, 2017Krawczyk KM, Hansson J, Nilsson H, Krawczyk KK, Swärd K, Johansson ME.

(20) TNFα drives pulmonary arterial hypertension by suppressing the BMP type-II receptor and altering NOTCH signallingNature communicationsJanuary 13, 2017Hurst LA, Dunmore BJ, Long L, Crosby A, Al-Lamki R, Deighton J, Southwood M, Yang X, Nikolic MZ, Herrera B, et al.

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Myosin IIb (D8H8) XP ® Rabbit mAb

Myosin IIb (D8H8) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

Citations (21)

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Myosin IIb (D8H8) XP ® Rabbit mAb from Cell Signaling Technology

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