Stat6 (D3H4) Rabbit mAb 5397S from Cell Signaling Technology

Product Specs
ItemStat6 (D3H4) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number5397S
Quantity100 µl
ApplicationsW, W-S, IP, ChIP
ReactivityH, M, R
ClonalityMonoclonal
TargetStat6
HostRabbit
NCBI Gene AliasesD12S1644, IL-4-STAT, STAT6B, STAT6C
NCBI Full Gene Namesignal transducer and activator of transcription 6
Research AreaImmunology and Inflammation
IsotypeIgG
CloneD3H4
Molecular Weight110

Questions and Answers

Can this antibody be used to study the role of STAT6 in macrophage polarization?

Yes, the Stat6 (D3H4) Rabbit mAb has been utilized in studies examining the role of STAT6 in macrophage polarization. For instance, it was shown that IL-4 derived from cancer cells activates STAT6, which is crucial for M2-like macrophage polarization, as indicated in research focusing on tumor-associated macrophages in pancreatic cancer ().

Is this antibody effective for Western blotting applications?

The Stat6 (D3H4) Rabbit mAb has been successfully employed in Western blotting, as evidenced by its use in various studies to detect STAT6 protein levels in different experimental contexts, including macrophage polarization and cancer research (, ).

What species can this antibody react with in experimental settings?

The Stat6 (D3H4) Rabbit mAb is reactive with multiple species, including humans, mice, and rats, making it suitable for a wide range of biological studies across different model organisms.

Has this antibody been cited in studies related to cancer research?

Yes, the Stat6 (D3H4) Rabbit mAb has been cited in numerous scientific articles, including those investigating its role in cancer-related signaling pathways, such as the JAK-STAT pathway in Ewing sarcoma and pancreatic cancer, highlighting its relevance in oncological research (, ).

Cell Signaling Technology

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Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Multi-omics analysis reveals the association of cesarean delivery with altered gut microbial profiles and a Th2-biased immune response in neonatesJournal of Translational MedicineMarch 11, 2026Chunhui Lai, Jiangang Zhang, Yu Xiong, Yijin Wang, Zhenxing Liu, Mingfang Shi, Shenglin Ye, Jianghui Zengmembrane, blocked, and probed with primary antibodies against STAT4 (Cat# 2653T, CST, USA) and STAT6 (Cat# 5397T, CST, USA), followed by incubation with appropriate HRP-conjugated secondary antibodies. Protein bands

(2) CXCL5 suppresses osteoclastogenesis and protects against lipoteichoic acid-induced bone loss by modulating PLCγ2 and c-Fos signalling in gram-positive periprosthetic joint infectionBone Joint ResMarch 4, 2026Yuhan Chang, Pei-Heng Jiang, Yung-Heng Hsu, Kee-Chin Sia, Steve Wen-Neng Ueng, Mei-Feng Chenof transcription 5 (STAT5) (Tyr694, 9359S), STAT5 (94205T), phospho-STAT6 (Tyr641, 56554S), STAT6 (5397S), phospho-FAK (Tyr397, 3283S), and FAK (3285T) (all from Cell Signaling Technology, USA); TRAP (ab191406)

(3) DNA-PK-mediated phosphorylation of STAT6 establishes a non-canonical type 2 immunity axis to prevent macrophage senescenceNature CommunicationsFebruary 24, 2026Zhao Zhou, Xinmeng Li, Yushuang Wang, Long Liang, Chunyang Wang, Yongchang Sun, Dongmei Wu, Yifu QiuHSP90 (Santa Cruz, #sc-13119, 1:10,000), α-Tubulin (Sigma, #T6199, 1:10,000), STAT6 (Cell Signaling, #5397, 1:1500), pSTAT6(pY641) (Cell Signaling, #56554, 1:1000), pSTAT6(pS807) (ABclonal Tech, customized,

(4) Role of the protease-activated receptor 2 in multi-walled carbon nanotube-induced macrophage polarization ex vivo and airway fibrosis in murine allergic lung disease in vivoFrontiers in ToxicologyFebruary 18, 2026Logan J. Tisch, Ryan D. Bartone, Silvio Antoniak, James C. Bonnerpurchased from Cell Signaling Technology, including phosphorylated (p)-STAT6 at Tyr640 #56554S; STAT6 #5397S; Arginase-1 #93668S; and β-actin #4967L. Bands were visualized using an Amersham Imager 680 (GE Life

(5) TGM2 Aggravates Acute Pancreatitis by Impairing Macrophage Efferocytosis Through Inhibition of the STAT6–GAS6 AxisAdvanced ScienceFebruary 17, 2026Xuxu Liu, Zhiwei Du, Liyi Wang, Zhihong Xie, Ziang Meng, Yi Zheng, Heming Wang, Yuanhang He, Dongbo Xueantibodies—TGM2 (Proteintech, 15100‐1‐AP, 1:20000), GAS6 (Proteintech, 13795‐1‐AP, 1:1000), STAT6 (CST, #5397, 1:1000), p‐STAT6 (CST, #56554, 1:1000), p‐JAK1 (CST, #3331, 1:1000) and p‐JAK3 (Proteintech, 29101‐1‐AP,

(6) Mechanotransduction-driven macrophage polarization via Integrin-SRC-STAT6 pathway in distraction osteogenesisJournal of Orthopaedic TranslationDecember 24, 2025Xu Yan, Haixing Wang, Xuan Lu, Linlong Li, Shanshan Bai, Zitong Li, Han Su, Rongjie Wu, Hui Chen, Yinuo Fan, et al.Cambridge, UK), Integrin-β (1:1000, #4706, Cell Signaling Technology, Danvers, USA), STAT6 (1:1000, # 5397, Cell Signaling Technology, Danvers, USA), p-STAT6 (1:1000, #56554, Cell Signaling Technology, Danvers,

(7) Tubule-derived CCN1 drives renal repair via αvβ5-STAT6-ARG1-dependent reprogramming of macrophagesCell Death & DiseaseDecember 21, 2025Ningxin Zhang, Chenyu Li, Yanlu Xin, Zhuo Song, Tianyang Li, Ruizhe Zhao, Minghao Gu, Lingyu Xu, Yanfei Wang, Xiaofei Man, et al.incubated overnight at 4°C with CCN1 (#39382), ARG1 (#93668), Phospho-STAT6 (Tyr641) (#9361), STAT6 (#5397) and GAPDH (#2118) primary antibodies (all 1:1000, Cell Signaling Technology). After washing, membranes

(8) L-Phenylalanine is a metabolic checkpoint of human Th2 cellsCell Reports MedicineDecember 16, 2025Abhijeet J. Kulkarni, Juan Rodriguez-Coira, Nino Stocker, Urszula Radzikowska, Antonio J. García-Cívico, María Isabel Delgado Dolset, Nuria Contreras, Inés Jardón Parages, Vanesa Saiz Sanchez, Pilar Serrano, et al.,Cell Signaling Technology,Cat#2983; RRID: AB_2105622 Stat6 (D3H4) Rabbit mAb,Cell Signaling Technology,Cat#5397; RRID: AB_11220421 Phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb,Cell Signaling Technology,Cat#5536;

(9) Inhibition of FKBP5 alleviates obstetric antiphospholipid syndrome by regulating macrophage polarizationScientific ReportsNovember 21, 2025Mingjie Song, Juan Wang, Wenli Mu, Yanwei Zheng, Rongzhen Jiang, Yanjun Cai, Yincheng Teng, Yu Xiaantibody (#9172, CST, Danvers, USA), anti-pSTAT1 antibody (AP0135, ABclonal, China), anti-STAT6 antibody (#5397, CST, Danvers, USA), anti-pSTAT6 antibody (#56554, CST, Danvers, USA), anti-PPARγ antibody (#2435, CST,

(10) Inhibition of protease-activated receptor-2 attenuates multi-walled carbon nanotube exacerbation of allergic lung disease in miceParticle and Fibre ToxicologyNovember 19, 2025Logan J. Tisch, Ryan D. Bartone, Silvio Antoniak, James C. Bonnerantibodies purchased from Cell Signaling Technology phosphorylated (p)-STAT6 at Tyr640, #56554S; STAT6, #5397S; Arginase-1, #93668S; and \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts}

(11) TRP75-mediated STAT3 activation promotes anti-apoptotic signaling and Ehrlichia chaffeensis infectionInfection and ImmunityNovember 11, 2025Nicholas A. Pittner, Jaclyn R. McCoy, Duc-Cuong Bui, Jere W. McBrideCell Signaling Technology), monoclonal rabbit α-STAT5 (94205S; Cell Signaling Technology), monoclonal rabbit α-STAT6 (5397S; Cell Signaling Technology), monoclonal rabbit α-phospho-STAT3 Y705 (9145S; Cell Signaling Technology),

(12) Baitong Decoction Ameliorates DSS-Induced Colitis via Modulation of STING and JAK/STAT PathwaysACS OmegaNovember 4, 2025Yuhang Zhou, XiaoXian Wang, Jingxing Lyu, Qingfeng Ruan, Xiaoxuan Zeng, Yuqi Li, Chuanqi Huang, Hongfeng Xu5483S), anti-IκB (CST, 4814S), antiphospho-IκB (CST, 2859S), anti-STAT3 (CST, 12640S), anti-STAT6 (CST, 5397S), anti-NF-κB (CST, 6956S), anti-JAK1 (CST, 3344S), antiphospho-JAK1 (CST, 3331S), anti-JAK2 (CST, 3230S),

(13) CTRP6 as a negative regulator of anti-inflammatory M2 macrophage polarizationImmunometabolism (Cobham)October 27, 2025Jeevotham Senthil Kumar, Emma Kempton, Muhammad Zubair Mehboob, Dingbo Lin, Xia Lei,Cell Signaling Technology®,1:500 AKT,9272S,Cell Signaling Technology®,1:1000 phospho-STAT6,56554S,Cell Signaling Technology®,1:500 STAT6,5397S,Cell Signaling Technology®,1:1000 phospho-ERK1/2 (p44/42),9101S,Cell Signaling Technology®,1:1000 ERK1/2

(14) Uncovering the individual immunotherapeutic roles of PTPN1 and PTPN2 in T cells during dual inhibitioniScienceOctober 17, 2025Luis-Alberto Perez-Quintero, Alexandre J. Poirier, Chu-Han Feng, Zuzet Martinez-Cordoba, Isabelle Aubry, Cedric Carli, Samaneh Kamyabiazar, Alain Pacis, Yevgen Zolotarov, Kelly-Anne Pike, et al.(Cat#4948; P173), STAT1 (Cat#9172T), STAT3 (Cat#4904), STAT4 (Cat#2653), STAT5a (Cat#94205), STAT6 (Cat#5397), pSTAT1 Y701 (Cat#7649), pSTAT3 Y705 (Cat#9131), pSTAT4 Y693(Cat#5267), pSTAT5 Y694 (Cat#9351) and

(15) Beyond Antagonism: IL-4 Exploits TNF signaling to Shape Its Gene Expression Signature in Monocytes and MacrophagesbioRxivJuly 31, 2025Ruoxi Yuan, Chao Yang, Bikash Mishra, David Oliver, Richard Bell, Lionel B. Ivashkivmin following previous development. The antibodies used were from Cell Signaling Technology: STAT6 (5397S), phosphor-STAT6 (9361S), C/EBPβ (3087S), HSP90α (8165S), IRF1 (8478S), from Sigma-Aldrich: α-Tubulin

(16) STAT1 and STAT6 orchestrate Cbs transcription and transsulfur metabolism in microglia and contribute to parkinson’s disease-related neuroinflammationCellular and Molecular Life SciencesJuly 30, 2025Yang Liu, Zhen Wang, Ya-Ting Ma, Yu-Xuan Chen, Xin-Yi Qian, Xiao-Ou Hou, Bo Wan, Hai-Gang Ren, Li-Fang Huγ-lyase (CSE) (SC-374249) was purchased from Santa Cruz. Antibodies against p-STAT6Tyr641, STAT6 (56554, 5397) and NLRP3 (15101) were purchased from Cell Signaling Technology (USA). Anti-FLAG (T0003) and anti-DJ-1

(17) Catalyst-modulated hydrogel dynamics for decoupling viscoelasticity and directing macrophage fate for diabetic wound healingBioact MaterJuly 5, 2025Yuening Mai, Honglei Wang, Jianyu Lu, Songsong Shi, Yixin Cai, Wei Zhang, Sujie Xie, Runzhi Huang, Shizhao Ji, Xue Qup-STAT3 (ab76315, Abcam), STAT3 (ab68153, Abcam), p-STAT6 (#9361, Cell Signaling Technology), STAT6 (#5397, Cell Signaling Technology), Arg1 (16001-1-AP, Proteintech), and GAPDH (AC002, ABclonal). HRP-conjugated

(18) Interleukin-4 prevents increased endothelial permeability by inducing pericyte survival and modulating microglial responses in diabetic retinopathyFront Endocrinol (Lausanne)July 2, 2025Jang-Hyuk Yunfollowing primary antibodies were used: anti-phospho-STAT6 (1:1000, CST; #9361), anti-STAT6 (1:1000, CST; #5397), anti-phospho-Akt (1:1000, CST; #4060), anti-Akt (1:1000, CST; #4691), anti-phospho-Erk1/2 (1:1000,

(19) ATF3-mediated inhibition of Trem2 by Toxoplasma gondii contributes to adverse pregnancy outcomesParasites & vectorsJuly 1, 2025Geng X, Yao T, Wang X, Wang J, Zhang T, Qian T, Liu C, Chen J.EPR22610-19, no. ab254268), STAT6 (D3H4) mAb (1:1000, rabbit monoclonal; CST, Danvers, MA, USA, D3H4, no. 5397), GAPDH antibody (1:50,000, mouse monoclonal; 1E6D9, no. 60004-1-Ig), HRP-conjugated goat anti-mouse

(20) Anti-IL-4Rα aptamer reduces IL-4 signalling and formation of nasal polypsERJ Open ResearchJune 16, 2025Fatemeh Saheb Sharif-Askari, Shirin Hafezi, Ibrahim Hachim, Bushra Mdkhana, Baraa Khalid, Salah Al-Sheakly, Narjes Saheb Sharif-Askari, Balachandar Selvakumar, Rabih Halwani(cat #5852, Cell Signaling Technology), p-STAT6 (cat #9361, Cell Signaling Technology), STAT6 (cat #5397, Cell Signaling Technology) and β-actin (cat #8457, Cell Signaling Technology), which served as a loading

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(1)

3/10/2023Good Stat6 (D3H4) Rabbit mAb for Western Blot Gherardo BaudoI used this antibody diluted 1:5000 (v/v) in 5% BSA TBST. The membrane was incubated overnight at 4 °C. Secondary antibody was diluted 1:10000. Results were great.
(1)

3/31/2017Stat6 Rabbit mAb Sachin SamuchiwalI studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's system using anti-mycobacterial ESAT6 binding peptides.

Stat6 (D3H4) Rabbit mAb

Stat6 (D3H4) Rabbit mAb from Cell Signaling Technology

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Stat6 (D3H4) Rabbit mAb from Cell Signaling Technology

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