APRIL/TNFSF13 Antibody (Aprily-5)

Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Breast cancer cells induce secretion of APRIL from neutrophils. APRIL stalk is clearly detectable in neutrophils but not in breast cancer cells. On the left panel, lysates of MCF7, T47D, Hs578T, and SKBR3 cells (60 ug each), as well as neutrophils (5 ug) were subjected to SDS-PAGE and western blotting using APRIL stalk antibody. On the right panel, neutrophils and T47D cells [non-transfected and transfected with pCR3 (T47D-pCR3) or pCR3-soluble APRIL (T47D-pCR3-sAPRIL)] were immunoblotted using Aprily-5 (Novus Biologicals: NBP1-97599; top panel) and reblotted using April ED (middle panel) antibodies. Aprily-5 detects soluble APRIL. The bottom panel shows Ponceau S staining of the membranes

Western Blot: APRIL/TNFSF13 Antibody (Aprily-5) [NBP1-97599] - Breast cancer cells induce secretion of APRIL from neutrophils. APRIL stalk is clearly detectable in neutrophils but not in breast cancer cells. On the left panel, lysates of MCF7, T47D, Hs578T, and SKBR3 cells (60 ug each), as well as neutrophils (5 ug) were subjected to SDS-PAGE and western blotting using APRIL stalk antibody. On the right panel, neutrophils and T47D cells [non-transfected and transfected with pCR3 (T47D-pCR3) or pCR3-soluble APRIL (T47D-pCR3-sAPRIL)] were immunoblotted using Aprily-5 (Novus Biologicals: NBP1-97599; top panel) and reblotted using April ED (middle panel) antibodies. Aprily-5 detects soluble APRIL. The bottom panel shows Ponceau S staining of the membranes