HIF-2 alpha/EPAS1 Antibody AF2886 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHIF-2 alpha/EPAS1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $479.00Supplier Page View Company Product Page
Catalog NumberAF2886
NCBI Gene AliasesHIF2A, MOP2, bHLHe73, ECYT4, HLF, PASD2
ApplicationsWestern Blot,Chromatin Immunoprecipitation (ChIP)
Conjugate/TagUnconjugated
IsotypeIgG
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
HostGoat
TargetHIF-2 alpha/EPAS1
ReactivityHuman,Rat
Quantity100 ug (also 25 ug)
NCBI Full Gene Nameendothelial PAS domain protein 1
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human HIF-2 alpha /EPAS1, Ser543-Thr870, Accession # Q99814
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human and rat HIF-2 alpha /EPAS1.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) HIF-2α accumulation in human monocytes upon transfer of hypoxia-associated miRNAs via plasma-derived small extracellular vesicles from head and neck cancer patientsFrontiers in OncologyJanuary 12, 2026Marie-Nicole Theodoraki, Linda Hofmann, Helen Bauer, Jonas Fleckner, Reinhard Depping, Christian Idel, Kirstin Plötze-Martin, Zuzana Penxova, Dirk Rades, Thomas K. Hoffmann, et al.Systems, Minneapolis, MN, USA; Cat: AF1935), anti-HIF-2α (1:1,000, R&D Systems, Minneapolis, MN, USA; Cat: AF2886), and anti-β-actin (1:5,000, Santa Cruz Biotechnology, Dallas, TX, USA) as described before (29). Molecular

(2) VHL Ser65 mutations enhance HIF2α signaling and promote epithelial-mesenchymal transition of renal cancer cellsCell & bioscienceMay 3, 2022Ma X, Tan Z, Zhang Q, Ma K, Xiao J, Wang X, Wang Y, Zhong M, Wang Y, Li J, et al.cocktail (Roche)) pre-washed Dynabead protein G (Invitrogen) was incubated with antibody against HIF2α (AF2886, R&D Systems). The fragmented chromatin lysate was precipitated with antibodies/beads complexes at 4

(3) Mitochondrial complex IV defects induce metabolic and signaling perturbations that expose potential vulnerabilities in HCT116 cellsFEBS open bioMay 1, 2022Uchenunu O, Zhdanov AV, Hutton P, Jovanovic P, Wang Y, Andreev DE, Hulea L, Papadopoli DJ, Avizonis D, Baranov PV, et al.Santa Cruz Biotechnologies (Dallas, TX, USA); SCO2 #PA5‐76209 from Thermo Fisher Scientific, HIF‐2α # AF2886 from R&D Systems (Minneapolis, MN, USA), SOD2 #ab16956, PDH E1‐α # Ab110330 and p‐PDH E1‐α (Ser293)

(4) New panel of biomarkers to discriminate between amelanotic and melanotic metastatic melanomaFrontiers in oncologyJanuary 1, 2022Militaru IV, Rus AA, Munteanu CVA, Manica G, Petrescu SM.Institute for Cancer Research, Lausanne Branch, Switzerland (16). Anti-HIF1α (AF1935) and anti-HIF2α (AF2886) antibodies were from R&D Systems. Anti-p44/42 MAP Kinase (ERK1/2) (4695), anti-Phospho-p44/42 MAPK

(5) Nonclinical Characterization of the Hypoxia-Inducible Factor Prolyl Hydroxylase Inhibitor Roxadustat, a Novel Treatment of Anemia of Chronic Kidney DiseaseThe Journal of pharmacology and experimental therapeuticsAugust 1, 2020Del Balzo U, Signore PE, Walkinshaw G, Seeley TW, Brenner MC, Wang Q, Guo G, Arend MP, Flippin LA, Chow FA, et al.610959, lot number 23681; BD Biosciences, San Jose, CA) or HIF-2a (1:1000 dilution, catalog number AF2886, lot number VEA0312021; R&D Systems, Minneapolis, MN) antibodies. The horseradish peroxidase (HRP)-conjugated

(6) Translational repression of HIF2α expression in mice with Chuvash polycythemia reverses polycythemiaThe Journal of clinical investigationApril 2, 2018Ghosh MC, Zhang DL, Ollivierre H, Eckhaus MA, Rouault TA.chamber. Hif2α antibody (affinity-purified goat anti-human) was obtained from R&D Systems (catalog AF2886), and a final concentration of 1.0 μg/ml was used as described in the R&D Systems protocol. Monoclonal

(7) Preferential activation of HIF-2α adaptive signalling in neuronal-like cells in response to acute hypoxiaPloS oneJanuary 1, 2017Martín-Aragón Baudel MAS, Rae MT, Darlison MG, Poole AV, Fraser JA.(Santa Cruz, #SC-1615), antiHIF1α (BD Sciences, #610958; Abcam, #ab1), anti-HIF-2α (1:500, R&D Systems, #AF2886), anti-HIF-3α (Acris antibodies, #AP20606PU-N), anti-CD44 (CST, #5640) or anti-Vimentin PLOS ONE |

(8) Cited2 modulates hypoxia-inducible factor-dependent expression of vascular endothelial growth factor in nucleus pulposus cells of the rat intervertebral discArthritis and rheumatismDecember 1, 2008Agrawal A, Gajghate S, Smith H, Anderson DG, Albert TJ, Shapiro IM, Risbud MV.

(9) Functional characterization of the 12p12.1 renal cancer-susceptibility locus implicates BHLHE41Nature communicationsJanuary 1, 0001"Bigot P, Colli LM, Machiela MJ, Jessop L, Myers TA, Carrouget J, Wagner S, Roberson D, Eymerit C, Henrion D, et al."were: anti-human HIF-1α (clone 54; BD Transduction Laboratories, San Jose, CA; 1:1,000), anti-HIF-2α (AF2886, R&D Systems, Minneapolis, MN; 1:1,000), anti-β-actin (NB600-505, Novus Biologicals, Littleton, CO;

(10) Identification of the Cysteine Protease Legumain as a Potential Chronic Hypoxia-Specific Multiple Myeloma Target GeneCellsJanuary 1, 0001"Clees AS, Stolp V, Häupl B, Fuhrmann DC, Wempe F, Seibert M, Weber S, Banning A, Tikkanen R, Williams R, et al."Novus biologicals (Bio-Techne GmbH, Wiesbaden, Germany) and a polyclonal goat antibody for HIF-2α (#AF2886) from R&D systems Inc. were used. A rabbit antibody against nucleolin was obtained from Santa Cruz Biotechnology

(11) Bafilomycin A1 activates HIF-dependent signalling in human colon cancer cells via mitochondrial uncouplingBioscience reportsJanuary 1, 0001"Zhdanov AV, Dmitriev RI, Papkovsky DB."Biologicals. Primary antibodies were against: α-tubulin (T5168, Sigma), HIF-1α and HIF-2α (MAB1536 and AF2886, R&D Systems), HIF-1α (NB100-105, Novus Biologicals), HIF-1β (ab2771, Abcam), HIF PHD2 (4835, Cell Signalling

(12) Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cellsScientific reportsJanuary 1, 0001"Li X, Lu J, Kan Q, Li X, Fan Q, Li Y, Huang R, Slipicevic A, Dong HP, Eide L, et al."CD44 (ab51037, Abcam), anti-integrin α2β3 (ab662, Abcam), anti-HIF1A (AF1935, R&D System), anti-HIF2A (AF2886, R&D System) and α-tubulin (T5168, Sigma). Statistical analyses For the experiments listed below at

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HIF-2 alpha/EPAS1 Antibody

HIF-2 alpha/EPAS1 Antibody

HIF-2 alpha/EPAS1 Antibody

HIF-2 alpha/EPAS1 Antibody

HIF-2 alpha/EPAS1 Antibody

HIF-2 alpha/EPAS1 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (12)

Reviews

HIF-2 alpha/EPAS1 Antibody from R&D Systems, a Bio-Techne Brand

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