Fig 1: Knockout of VCAM1 in oligodendrocytes decreases myelin marker protein expression in mice.(a,b) MBP-Cre-driven VCAM1 conditional knockout (VCAM1fl/fl; Mbp-Cre) and control mouse spinal cord cross sections at postnatal day 7 were stained with an antibody against GFAP (green) or NeuN (red). Data are representative. Scale bar, 200 µm (a); 100 µm (b). (c) The intensity of GFAP staining per one square millimetre was semi-quantified at postnatal days 7, 14 and 21. Data were evaluated using Student’s t-test (non-significance; n=6 slices of two independent experiments). (d) The number of NeuN+ cells per field was counted. Data were evaluated using Student’s t-test (non-significance; n=5 slices of two independent experiments). (e) Spinal cord cross sections of VCAM1fl/fl; Mbp-Cre and control mice at postnatal day 7 were co-stained with an antibody against CC1 (red) and DAPI (blue). Data are representative. Scale bar, 100 µm. (f,g) Antibodies against CC1 (red) and Olig2 (green) were used for co-staining in spinal cord cross sections at postnatal days 7, 14, 21 and 28. Data are representative. Scale bar, 100 µm. The percentage of CC1+ cells among the Olig2+ cells is shown. Data were evaluated using Student’s t-test (**P=0.00638 (P7), 0.000961 (P14), 0.00111 (P21) or 2.11E-09 (P28); n=6–10 slices of two independent experiments). (h,i) Corpus callosum sections of VCAM1fl/fl; Mbp-Cre and control mouse (h) or VCAM1fl/fl; Ng2-Cre and control mouse (i) at postnatal day 11 were co-stained with an anti-MBP antibody (red) and DAPI (blue). Data are representative of six slices of two independent experiments. Scale bar, 200 µm. (j) Tissue lysates from 7-day-old spinal cords or 11-day-old brains were immunoblotted with an antibody against MBP, CNPase, VCAM1, CD69, MAG, Olig2, Sox10, Ascl1, Nkx2.1 or actin. Data are representative of three experiments.