Anti-AHR antibody [RPT1] GTX22770 from GeneTex

Product Specs
ItemAnti-AHR antibody [RPT1]
CompanyGeneTex
Price $419.00Supplier Page
Catalog NumberGTX22770
ApplicationsWB, ICC/IF, IHC-P, IP, ELISA, ChIP assay
ReactivityHuman, Mouse, Rat, Pig, Primate
ClonalityMonoclonal
TargetAHR
IsotypeIgG1
HostMouse
ImmunogenSynthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of human AhR
Quantity100 µl
Conjugate/TagUnconjugated
FormatPBS, 0.1% BSA, 0.05% Sodium azide
Antibody TypePrimary Antibodies
CloneRPT1
NCBI Full Gene Namearyl hydrocarbon receptor
NCBI Gene Aliasesaryl hydrocarbon receptor, bHLHe76, bHLHe76, RP85
Research AreaCell Biology
PurityProtein G purified
Gene NameAHR
Molecular Weight96
Concentration1mg/ml

Questions and Answers

Can this antibody be used for immunofluorescence applications, and if so, how has it been utilized in research?

Yes, the Anti-AHR antibody [RPT1] has been shown to work in immunofluorescence applications. For instance, in a study, cells were fixed and permeabilized before being incubated with the antibody overnight at 4 °C, followed by detection with a secondary antibody. This method allowed for the visualization of AHR localization in cells using confocal microscopy, demonstrating its utility in studying AHR dynamics in cellular contexts.

What species does this antibody recognize, and is it effective across different models?

The Anti-AHR antibody [RPT1] recognizes multiple species, including human, mouse, rat, pig, and non-human primates. This broad reactivity makes it suitable for various experimental models, as evidenced by its application in studies involving macrophages and uterine leiomyoma cells, where it facilitated the investigation of AHR's role in inflammation and cell survival.

Has this antibody been cited in any significant studies, and what were the findings related to AHR?

Yes, the Anti-AHR antibody [RPT1] has been cited in at least ten scientific articles. For example, one study identified the role of AHR in promoting macrophage survival and bactericidal activity through its upregulation by a novel modulator, Punicalagin. This highlights the antibody's relevance in exploring AHR's involvement in immune responses and potential therapeutic targets.

What specific applications has this antibody been validated for in the literature?

The Anti-AHR antibody [RPT1] has been validated for several applications, including Western blotting, immunocytochemistry, immunohistochemistry, immunoprecipitation, ELISA, and ChIP assays. Its versatility allows researchers to utilize it in various experimental setups to investigate the function and regulation of the aryl hydrocarbon receptor in different biological contexts.

GeneTex

GeneTex, Inc. 2456 Alton Parkway

Irvine, California 92606

United States

Phone: 1-877-GeneTex (436-3839) or 949-553-1900
Fax: 949-309-2888

Company Profile

Website: http://www.genetex.com

(1) Kynurenine promotes porcine intestinal epithelial cell proliferation by activating the AHR-MST1-YAP1 axisJ Anim Sci BiotechnolMarch 17, 2026Zhenguo Hu, Lanmei Yin, Qianqian Wang, Junhao Deng, Xiaofeng Zhu, Huansheng Yang, Pengpeng Zhang, Yulong Yin, Xiongzhuo Tangovernight at 4 °C with primary antibodies against PCNA (60097-1-Ig, Proteintech, China) and AHR (1:300, GTX22770, GeneTex, USA), followed by incubation with Alexa Fluor 488-conjugated or Alexa Fluor 594-conjugated

(2) Punicalagin as a novel selective aryl hydrocarbon receptor (AhR) modulator upregulates AhR expression through the PDK1/p90RSK/AP-1 pathway to promote the anti-inflammatory response and bactericidal activity of macrophagesCell communication and signaling : CCSOctober 3, 2024Dai W, Yin S, Wang F, Kuang T, Xiao H, Kang W, Yun C, Wang F, Luo L, Ao S, et al.Triton X-100, and blocked for 60 min with QuickBlock™ Blocking Buffer. Cells were incubated with AhR (GTX22770, GeneTex) or Phospho-ERK (4370, CST) antibodies overnight at 4 °C, then incubated with Alexa Fluor 647-conjugated

(3) Gut microbiota-derived tryptophan metabolites alleviate liver injury via AhR/Nrf2 activation in pyrrolizidine alkaloids-induced sinusoidal obstruction syndromeCell & bioscienceJuly 8, 2023Shang H, Huang C, Xiao Z, Yang P, Zhang S, Hou X, Zhang L.milk for 1 h and incubated overnight with specific antibodies against MMP-9 (ab76003, Abcam), AhR (GTX22770, GeneTex), CYP1A1(GTX55582, GeneTex), Nrf2 (PA5-88084, Thermofisher), Lamin B1(YT5180, Immunoway), and

(4) Mono-(2-ethyl-5-hydroxyhexyl) phthalate promotes uterine leiomyoma cell survival through tryptophan-kynurenine-AHR pathway activationProceedings of the National Academy of Sciences of the United States of AmericaNovember 22, 2022Iizuka T, Yin P, Zuberi A, Kujawa S, Coon JS 5th, Björvang RD, Damdimopoulou P, Pacyga DC, Strakovsky RS, Flaws JA, et al.ice. After blocking with 2% BSA in PBS for 30 min, the slides were incubated with antibody (1/100, GTX22770, RPT1, GeneTex) overnight at 4 °C. Then, the slides were incubated for 1 h with secondary antibody Alexa

(5) NUPR1 contributes to radiation resistance by maintaining ROS homeostasis via AhR/CYP signal axis in hepatocellular carcinomaBMC medicineOctober 19, 2022Zhan Y, Zhang Z, Liu Y, Fang Y, Xie Y, Zheng Y, Li G, Liang L, Ding Y.Antibodies used in the study were anti-Flag (F1804, Sigma-Aldrich), NUPR1 (15056-1-AP, Proteintech), AhR (GTX22770, GeneTex), and AhR (A4000, ABclonal). Mouse IgG (B900620) and secondary antibody HRP-goat anti-rabbit

(6) The Host CYP1A1-Microbiota Metabolic Axis Promotes Gut Barrier Disruption in Methicillin-Resistant Staphylococcus aureus-Induced Abdominal SepsisFrontiers in microbiologyJanuary 1, 2022Ma X, Jin H, Chu X, Dai W, Tang W, Zhu J, Wang F, Yang X, Li W, Liu G, et al.

(7) TDO2 and tryptophan metabolites promote kynurenine/AhR signals to facilitate glioma progression and immunosuppressionAmerican journal of cancer researchJanuary 1, 2022Zhong C, Peng L, Tao B, Yin S, Lyu L, Ding H, Yang X, Peng T, He H, Zhou P.

(8) Exposing human primary dermal fibroblasts to particulate matter induces changes associated with skin agingFASEB journal : official publication of the Federation of American Societies for Experimental BiologyNovember 1, 2020Reynolds WJ, Hanson PS, Critchley A, Griffiths B, Chavan B, Birch-Machin MA.

(9) IL4I1 Is a Metabolic Immune Checkpoint that Activates the AHR and Promotes Tumor ProgressionCellSeptember 3, 2020Sadik A, Somarribas Patterson LF, Öztürk S, Mohapatra SR, Panitz V, Secker PF, Pfänder P, Loth S, Salem H, Prentzell MT, et al.

(10) Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissuesLaboratory investigation; a journal of technical methods and pathologySeptember 1, 2016Mariuzzi L, Domenis R, Orsaria M, Marzinotto S, Londero AP, Bulfoni M, Candotti V, Zanello A, Ballico M, Mimmi MC, et al.Aczon Biotech, Bologna, IT; dilution for IHC 1:50, incubation 1 h at 37°C); mouse AhR (clone RPT1, GTX22770, GeneTex, Irvine CA, USA; dilution for IHC 1:100, incubation 16 hr at 4°C); rabbit AhR (LS-A3391, LifeSpan BioSciences

(11) Aryl hydrocarbon receptor-ligand axis mediates pulmonary fibroblast migration and differentiation through increased arachidonic acid metabolismToxicologyAugust 31, 2016Su HH, Lin HT, Suen JL, Sheu CC, Yokoyama KK, Huang SK, Cheng CM.

(12) Severe liver cirrhosis markedly reduces AhR-mediated induction of cytochrome P450 in rats by decreasing the transcription of target genesPloS oneJanuary 1, 2013Floreani M, De Martin S, Gabbia D, Barbierato M, Nassi A, Mescoli C, Orlando R, Bova S, Angeli P, Gola E, et al.

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Anti-AHR antibody [RPT1]

Anti-AHR antibody [RPT1]

Anti-AHR antibody [RPT1]

Anti-AHR antibody [RPT1]

Anti-AHR antibody [RPT1] from GeneTex

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Questions and Answers

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Anti-AHR antibody [RPT1] from GeneTex

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