FCCP, mitochondrial oxidative phosphorylation uncoupler ab120081 from Abcam

Product Specs
ItemFCCP, mitochondrial oxidative phosphorylation uncoupler
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab120081
Quantity10 mg, 50 mg
Purity0.99
TypeChemical
Molecule NameCarbonyl cyanide p-trifluoromethoxyphenylhydrazone

Questions and Answers

Can this mitochondrial uncoupler be used to measure oxygen consumption rates in cancer cells?

Yes, FCCP has been utilized in studies to measure oxygen consumption rates (OCR) in cancer cells, specifically in head and neck squamous cell carcinoma and oesophageal squamous cell carcinoma cell lines. It was used as a positive control in experiments assessing metabolic differences between cancerous and normal cells, confirming its effectiveness in such applications ().

How does FCCP affect mitochondrial function in the context of neurodegenerative diseases?

FCCP has been employed in research to induce mitochondrial stress in human brain endothelial cells, leading to increased oxygen consumption and cytosolic DNA leakage. This suggests that FCCP can be used to study mitochondrial dysfunction and its implications in neuroinflammation associated with neurodegenerative diseases ().

Is FCCP effective in assessing metabolic changes in tumor cells?

Yes, FCCP has been shown to be effective in assessing metabolic changes in tumor cells. In a study involving the BALB/c tumor therapy model, FCCP was used to measure the oxygen consumption rate and extracellular acidification rate, indicating its utility in evaluating metabolic phenotypes of tumor cells ().

What precautions should be taken when handling FCCP in experiments?

FCCP is known to be acutely toxic even at low doses, so it is essential to wear personal protective equipment, including gloves and protective clothing, while handling this reagent ().

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(1) Rab12 is a regulator of mitophagy and mitochondrial homeostasisbioRxivMarch 31, 2026Tara Richbourg, Alex George, Anas Bitar, Ian T. Ryde, Casey Farrell, Tuyana Malankhanova, Jennifer Liu, Silas A. Buck, Ivana Barraza, So Young A Kim, et al.described below. For experiments involving mitophagy induction, we treated cultures with 30 μM FCCP (Abcam, ab120081) or a v/v DMSO vehicle control (Sigma-Aldrich, D2650) in complete medium for 4 h prior to imaging. Rab

(2) CD93-targeted resveratrol-loaded PLGA nanoparticles remodel CD8⁺ T cell metabolism through AIF-mediated oxidative phosphorylation to overcome lung cancer immunotherapy resistanceJournal of NanobiotechnologyMarch 14, 2026Zhou Jiang, Yuning Li(OCR) was measured by sequential injection of oligomycin (1 µM; ab141829, Abcam, USA), FCCP (1 µM; ab120081, Abcam, USA), and rotenone/antimycin A (0.5 µM; ab147494, Abcam, USA). Extracellular acidification rate

(3) Zonal hepatocellular responses to acute ethanol consumption: impacts on mitochondrial function and liver metabolismToxicological SciencesMarch 2, 2026Matthew T. Savoca, Kenji Takemoto, Jiangting Hu, Li Li, Zhi Zhong, John J. LemastersCayman Chemical, Ann Arbor, MI, USA), carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP, 1μM, ab120081, Abcam), and rotenone/antimycin A (1μM each, 557368, A8674, Sigma Aldrich). Wells were imaged using

(4) Biallelic variants in COX18 cause a mitochondrial disorder primarily manifesting as peripheral neuropathyBrainJanuary 8, 2026Camila Armirola-Ricaurte, Laura Morant, Isabelle Adant, Sherifa A Hamed, Menelaos Pipis, Stephanie Efthymiou, Silvia Amor-Barris, Derek Atkinson, Liedewei Van de Vondel, Aleksandra Tomic, et al.perchrolate, Enzo Life Sciences) for 30 min at 37°C. Positive control cells were incubated with 20 nM FCCP (ab120081, Abcam) for 5 min at 37°C before staining. After staining, cells were rinsed with prewarmed PBS and

(5) Hypoxia regulates Th17/Treg balance by altering chromatin accessibility and gene expressionThe FEBS JournalDecember 23, 2025Mariana Cázares‐Olivera, Shiyang Song, Sofia Ylinen, Talha Qureshi, Bin Li, Zhi Chensequential injections of Oligomycin (1.5 μm, cat. no.: O4876‐5MG; Sigma‐Aldrich), FCCP (1 μm, cat. no.: ab120081; Abcam; Cambridge, UK), FCCP (0.5 μm), Rotenone (0.5 μm, cat. no.: R8875‐1G; Sigma‐Aldrich) + Antimycin

(6) Impact of Acute Myeloid Leukemia Cells on the Metabolic Function of Bone Marrow Mesenchymal Stem CellsInternational Journal of Molecular SciencesAugust 27, 2025Helal Ahmed, Pradeep Kumar Patnana, Yahya S. Al-Matary, Maren Fiori, Jan Vorwerk, Marah H. Ahmad, Eva Dazert, Lorenz Oelschläger, Axel Künstner, Bertram Opalka, et al.and measured directly by flow cytometry. Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) (ab120081, Abcam) was used as a negative control for TMRE staining. Mitochondrial mass was detected by resuspending

(7) Metabolic reprogramming of the neovascular niche promotes regenerative angiogenesis in proliferative retinopathyNat CommunJune 25, 2025Gael Cagnone, Sheetal Pundir, Charlotte Betus, Tapan Agnihotri, Anli Ren, Jin Sung -Kim, Noémie-Rose Harvey, Emilie Heckel, Mei Xi Chen, Anu Situ, et al.(Agilent) consisted of sequential injections of oligomycin (3 µM, O4876, Sigma-Aldrich, USA), FCCP (2 µM, ab120081, Abcam, USA), and rotenone (1 µM, R887, Sigma-Aldrich, USA)–antimycin A (1 µM, A8674, Sigma-Aldrich,

(8) Increased burden of rare risk variants across gene expression networks predisposes to sporadic Parkinson''s diseaseCell reportsMay 27, 2025Eubanks E, VanderSleen K, Mody J, Patel N, Sacks B, Farahani MD, Wang J, Elliott J, Jaber N, Akçimen F, et al.were used to inhibit complex V, 5uM of rotenone (#R8875–5G, Sigma) to inhibit complex I and 1uM FCCP (#ab120081, Abcam) to completely depolarize the mitochondria. Experiments were imaged on a Zeiss LSM800 confocal

(9) DNA damage response regulator ATR licenses PINK1-mediated mitophagyNucleic Acids ResMarch 19, 2025Christian Marx, Xiaobing Qing, Yamin Gong, Joanna Kirkpatrick, Kanstantsin Siniuk, Galina V Beznoussenko, Gururaj Rao Kidiyoor, Murat Kirtay, Katrin Buder, Philipp Koch, et al.H8627; all from Sigma–Aldrich), 2 μM of carbonylcyanide 4-(trifluoromethoxy)-phenylhydrazone (FCCP; ab120081), 10 μM of MitoParaquat (MitoPQ; ab146819), 10 μM of MitoTempol (MitoT; ab144644; all from Abcam), 10

(10) Germline Single-Nucleotide Polymorphism GFI1-36N Causes Alterations in Mitochondrial Metabolism and Leads to Increased ROS-Mediated DNA Damage in a Murine Model of Human Acute Myeloid LeukemiaBiomedicinesJanuary 5, 2025Jan Vorwerk, Longlong Liu, Theresa Helene Stadler, Daria Frank, Helal Mohammed Mohammed Ahmed, Pradeep Kumar Patnana, Maxim Kebenko, Eva Dazert, Bertram Opalka, Nikolas von Bubnoff, et al.For negative control in TMRE staining, carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP) (#ab120081, Abcam) was used. Mito-mass was analyzed by resuspending 0.25 × 106 cells in prewarmed (37 °C) cell culture media

(11) MitoTracker Red for Isolation of Zone-Specific Hepatocytes and Characterization of Hepatic Sublobular Metabolism§Biochemical and Biophysical Research CommunicationsNovember 1, 2024Matthew Savoca, Kenji Takemoto, Jiangting Hu, Li Li, Jacob Jacob Kendrick, Zhi Zhong, John J. LemastersCayman Chemical, Ann Arbor, MI, USA), 1 μM carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP, ab120081, Abcam), and 1 μM rotenone (557368, Sigma Aldrich)/1 μM antimycin a (A8674, Sigma Aldrich). Individual

(12) Inflammation-induced epigenetic imprinting regulates intestinal stem cellsCell Stem CellOctober 3, 2024Dongchang Zhao, Visweswaran Ravikumar, Tyler J. Leach, Daniel Kraushaar, Emma Lauder, Lu Li, Yaping Sun, Katherine Oravecz-Wilson, Evan T. Keller, Fengju Chen, et al.Express Enzyme,Thermo Fisher,Cat# 12604013 Oligomycin A,MilliporeSigma,Cat# 75351-5mg FCCP,Abcam,Cat# ab120081 Antimycin,MilliporeSigma,Cat# A8674-25mg Rotenone,Tocris,Cat# 3616 Seahorse XF base medium,Agilent

(13) Alternative splicing expands the clinical spectrum of NDUFS6-related mitochondrial disordersGenetics in medicine : official journal of the American College of Medical GeneticsJune 1, 2024Armirola-Ricaurte C, Zonnekein N, Koutsis G, Amor-Barris S, Pelayo-Negro AL, Atkinson D, Efthymiou S, Turchetti V, Dinopoulos A, Garcia A, et al.incubated cells from healthy individuals with 20 nM carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (ab120081, Abcam) for 5 minutes at 37 °C to depolarize the mitochondrial membrane before staining. After staining,

(14) Interleukin 21 Drives a Hypermetabolic State and CD4+ T-Cell–Associated Pathogenicity in Chronic Intestinal InflammationGastroenterologyMay 1, 2024Adebowale O. Bamidele, Shravan K. Mishra, Guilherme Piovezani Piovezani Ramos, Petra Hirsova, Emily E. Klatt, Leena M. Abdelrahman, Mary R. Sagstetter, Heidi M. Davidson, Patrick J. Fehrenbach, Lucía Valenzuela-Pérez, et al.,Thermo Fisher Scientific,Cat#M7512 ER-Tracker™ Blue-White DPX,Thermo Fisher Scientific,Cat#E12353 Oligomycin,Abcam,Cat#ab141829 FCCP,Abcam,Cat#ab120081 Rotenone,Abcam,Cat#ab143135 Antimycin A,Abcam,Cat#ab141904 2-deoxyglucose (2-DG),APEXBIO,Cat#B1027 Seahorse

(15) THC and sperm: Impact on fertilization capability, pre-implantation in vitro development and epigenetic modificationsPLoS OneMarch 27, 2024Alexander G. Kuzma-Hunt, Reem Sabry, Ola S. Davis, Vivien B. Truong, Jibran Y. Khokhar, Laura A. Favettacontrol group was treated with 0.132mM of carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) (Abcam; ab120081)–a potent mitochondrial oxidative phosphorylation uncoupler. After the 6-hour treatment period, 200μL

(16) G2019S selective LRRK2 kinase inhibitor abrogates mitochondrial DNA damageNPJ Parkinsons DisMarch 1, 2024Nicholas Pena, Tara Richbourg, Claudia P. Gonzalez-Hunt, Rui Qi, Paul Wren, Carrolee Barlow, Natalie F. Shanks, Holly J. Carlisle, Laurie H. Sandersfollowed by three washes with serum-free medium. Complete medium was used to prepare a 20 µM FCCP (Abcam, ab120081) or DMSO control treatment solution, which was used to treat cells for 3 h. Following treatment, cells

(17) Thioflavin T indicates mitochondrial membrane potential in mammalian cellsBiophysical ReportsDecember 13, 2023Emily Skates, Hadrien Delattre, Zoe Schofield, Munehiro Asally, Orkun S. Soyerwas stored at –20°C in the dark. Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP, Abcam ab120081) was dissolved in DMSO to make a 1 mM stock and stored at –20°C in the dark. Ascorbic acid (Sigma Aldrich,

(18) N-acetylneuraminate pyruvate lyase controls sialylation of muscle glycoproteins essential for muscle regeneration and functionScience advancesJune 30, 2023Da Silva A, Dort J, Orfi Z, Pan X, Huang S, Kho I, Heckel E, Muscarnera G, van Vliet PP, Sturiale L, et al.(Sigma-Aldrich, catalog no. O4876), 400 nM carbonyl cyanide-p-trifluoromethoxyphenyl-hydrazon (Abcam, catalog no. ab120081), and 10 nM sodium pyruvate (Gibco, catalog no. 11360070), followed by 1 μM antimycin A (Sigma-Aldrich,

(19) Organelle interactions compartmentalize hepatic fatty acid trafficking and metabolismCell reportsMay 30, 2023Najt CP, Adhikari S, Heden TD, Cui W, Gansemer ER, Rauckhorst AJ, Markowski TW, Higgins L, Kerr EW, Boyum MD, et al.Grade",Promega,V5280 MitoTracker Deep Red,Invitrogen,M22426 [14C]oleate,Perkin Elmer,NEC317250UC FCCP,Abcam,Ab120081 TMRE,Abcam,Ab113852 Etomoxir,Abcam,Ab254445 [13C]palmitate,Sigma-Aldrich,687871–3.80G Ponceau S,Fisher

(20) STING-Triggered CNS Inflammation in Human Neurodegenerative DiseasesBiomedicinesMay 5, 2023Ferecskó AS, Smallwood MJ, Moore A, Liddle C, Newcombe J, Holley J, Whatmore J, Gutowski NJ, Eggleton P.fatty acid oxidation (FAO) activator, FCCP (carbonyl cyanide-4-(trifluormethoxy) phenylhydrazone, Abcam—ab120081), was subsequently supplemented into the wells as a positive control, or 1 μL (2.5 μM—final concentration)

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FCCP, mitochondrial oxidative phosphorylation uncoupler from Abcam

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FCCP, mitochondrial oxidative phosphorylation uncoupler from Abcam

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