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FCCP, mitochondrial oxidative phosphorylation uncoupler
FCCP, mitochondrial oxidative phosphorylation uncoupler from Abcam
Be the first to write a review!
Citations:
(39)
Supplier Page
Supplier Page from
Abcam for
FCCP, mitochondrial oxidative phosphorylation uncoupler
Description
Potent mitochondrial oxidative phosphorylation uncoupler
Product Specs
Q&A
Company Info
Citations
Reviews
Product Specs
Item
FCCP, mitochondrial oxidative phosphorylation uncoupler
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab120081
Quantity
10 mg, 50 mg
Purity
0.99
Type
Chemical
Molecule Name
Carbonyl cyanide p-trifluoromethoxyphenylhydrazone
Add to Compare List
Questions and Answers
Can this mitochondrial uncoupler be used to measure oxygen consumption rates in cancer cells?
Yes, FCCP has been utilized in studies to measure oxygen consumption rates (OCR) in cancer cells, specifically in head and neck squamous cell carcinoma and oesophageal squamous cell carcinoma cell lines. It was used as a positive control in experiments assessing metabolic differences between cancerous and normal cells, confirming its effectiveness in such applications (
blanckes2021
).
How does FCCP affect mitochondrial function in the context of neurodegenerative diseases?
FCCP has been employed in research to induce mitochondrial stress in human brain endothelial cells, leading to increased oxygen consumption and cytosolic DNA leakage. This suggests that FCCP can be used to study mitochondrial dysfunction and its implications in neuroinflammation associated with neurodegenerative diseases (
ferecsko2023
).
Is FCCP effective in assessing metabolic changes in tumor cells?
Yes, FCCP has been shown to be effective in assessing metabolic changes in tumor cells. In a study involving the BALB/c tumor therapy model, FCCP was used to measure the oxygen consumption rate and extracellular acidification rate, indicating its utility in evaluating metabolic phenotypes of tumor cells (
meyer2021
).
What precautions should be taken when handling FCCP in experiments?
FCCP is known to be acutely toxic even at low doses, so it is essential to wear personal protective equipment, including gloves and protective clothing, while handling this reagent (
koopman2016
).
Ask a Question
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (39)
(1)
Rab12 is a regulator of mitophagy and mitochondrial homeostasis
bioRxiv
March 31, 2026
Tara Richbourg, Alex George, Anas Bitar, Ian T. Ryde, Casey Farrell, Tuyana Malankhanova, Jennifer Liu, Silas A. Buck, Ivana Barraza, So Young A Kim,
et al.
described below. For experiments involving mitophagy induction, we treated cultures with 30 μM
FCCP
(
Abcam
,
ab120081
) or a v/v DMSO vehicle control (
Sigma-Aldrich
,
D2650
) in complete medium for 4 h prior to imaging. Rab
(2)
CD93-targeted resveratrol-loaded PLGA nanoparticles remodel CD8⁺ T cell metabolism through AIF-mediated oxidative phosphorylation to overcome lung cancer immunotherapy resistance
Journal of Nanobiotechnology
March 14, 2026
Zhou Jiang, Yuning Li
(OCR) was measured by sequential
injection
of oligomycin (1 µM;
ab141829
,
Abcam
, USA),
FCCP
(1 µM;
ab120081
,
Abcam
, USA), and rotenone/antimycin A (0.5 µM; ab147494, Abcam, USA). Extracellular acidification rate
(3)
Zonal hepatocellular responses to acute ethanol consumption: impacts on mitochondrial function and liver metabolism
Toxicological Sciences
March 2, 2026
Matthew T. Savoca, Kenji Takemoto, Jiangting Hu, Li Li, Zhi Zhong, John J. Lemasters
Cayman Chemical
, Ann Arbor, MI, USA), carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP, 1μM,
ab120081
,
Abcam
), and rotenone/antimycin A (1μM each, 557368,
A8674
,
Sigma Aldrich
). Wells were imaged using
(4)
Biallelic variants in COX18 cause a mitochondrial disorder primarily manifesting as peripheral neuropathy
Brain
January 8, 2026
Camila Armirola-Ricaurte, Laura Morant, Isabelle Adant, Sherifa A Hamed, Menelaos Pipis, Stephanie Efthymiou, Silvia Amor-Barris, Derek Atkinson, Liedewei Van de Vondel, Aleksandra Tomic,
et al.
perchrolate,
Enzo Life Sciences
) for 30 min at 37°C. Positive control cells were incubated with 20 nM
FCCP
(
ab120081
,
Abcam
) for 5 min at 37°C before
staining
. After
staining
, cells were rinsed with prewarmed PBS and
(5)
Hypoxia regulates Th17/Treg balance by altering chromatin accessibility and gene expression
The FEBS Journal
December 23, 2025
Mariana Cázares‐Olivera, Shiyang Song, Sofia Ylinen, Talha Qureshi, Bin Li, Zhi Chen
sequential
injections
of
Oligomycin
(1.5 μm, cat. no.:
O4876
‐5MG;
Sigma‐Aldrich
),
FCCP
(1 μm, cat. no.:
ab120081
;
Abcam
; Cambridge, UK), FCCP (0.5 μm),
Rotenone
(0.5 μm, cat. no.: R8875‐1G;
Sigma‐Aldrich
) + Antimycin
(6)
Impact of Acute Myeloid Leukemia Cells on the Metabolic Function of Bone Marrow Mesenchymal Stem Cells
International Journal of Molecular Sciences
August 27, 2025
Helal Ahmed, Pradeep Kumar Patnana, Yahya S. Al-Matary, Maren Fiori, Jan Vorwerk, Marah H. Ahmad, Eva Dazert, Lorenz Oelschläger, Axel Künstner, Bertram Opalka,
et al.
and measured directly by
flow cytometry
. Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (
FCCP
) (
ab120081
,
Abcam
) was used as a negative control for TMRE
staining
. Mitochondrial mass was detected by resuspending
(7)
Metabolic reprogramming of the neovascular niche promotes regenerative angiogenesis in proliferative retinopathy
Nat Commun
June 25, 2025
Gael Cagnone, Sheetal Pundir, Charlotte Betus, Tapan Agnihotri, Anli Ren, Jin Sung -Kim, Noémie-Rose Harvey, Emilie Heckel, Mei Xi Chen, Anu Situ,
et al.
(Agilent) consisted of sequential
injections
of oligomycin (3 µM,
O4876
,
Sigma-Aldrich
, USA),
FCCP
(2 µM,
ab120081
,
Abcam
, USA), and rotenone (1 µM, R887, Sigma-Aldrich, USA)–antimycin A (1 µM,
A8674
,
Sigma-Aldrich
,
(8)
Increased burden of rare risk variants across gene expression networks predisposes to sporadic Parkinson''s disease
Cell reports
May 27, 2025
Eubanks E, VanderSleen K, Mody J, Patel N, Sacks B, Farahani MD, Wang J, Elliott J, Jaber N, Akçimen F,
et al
.
were used to
inhibit
complex V, 5uM of rotenone (#R8875–5G, Sigma) to
inhibit
complex I and 1uM
FCCP
(#
ab120081
,
Abcam
) to completely depolarize the
mitochondria
. Experiments were imaged on a Zeiss LSM800
confocal
(9)
DNA damage response regulator ATR licenses PINK1-mediated mitophagy
Nucleic Acids Res
March 19, 2025
Christian Marx, Xiaobing Qing, Yamin Gong, Joanna Kirkpatrick, Kanstantsin Siniuk, Galina V Beznoussenko, Gururaj Rao Kidiyoor, Murat Kirtay, Katrin Buder, Philipp Koch,
et al.
H8627; all from Sigma–Aldrich), 2 μM of carbonylcyanide 4-(trifluoromethoxy)-phenylhydrazone (
FCCP
;
ab120081
), 10 μM of MitoParaquat (
MitoPQ
;
ab146819
), 10 μM of
MitoTempol
(MitoT;
ab144644
; all from
Abcam
), 10
(10)
Germline Single-Nucleotide Polymorphism GFI1-36N Causes Alterations in Mitochondrial Metabolism and Leads to Increased ROS-Mediated DNA Damage in a Murine Model of Human Acute Myeloid Leukemia
Biomedicines
January 5, 2025
Jan Vorwerk, Longlong Liu, Theresa Helene Stadler, Daria Frank, Helal Mohammed Mohammed Ahmed, Pradeep Kumar Patnana, Maxim Kebenko, Eva Dazert, Bertram Opalka, Nikolas von Bubnoff,
et al.
For negative control in TMRE
staining
, carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (
FCCP
) (#
ab120081
,
Abcam
) was used. Mito-mass was analyzed by resuspending 0.25 × 106 cells in prewarmed (37 °C)
cell culture media
(11)
MitoTracker Red for Isolation of Zone-Specific Hepatocytes and Characterization of Hepatic Sublobular Metabolism§
Biochemical and Biophysical Research Communications
November 1, 2024
Matthew Savoca, Kenji Takemoto, Jiangting Hu, Li Li, Jacob Jacob Kendrick, Zhi Zhong, John J. Lemasters
Cayman Chemical
, Ann Arbor, MI, USA), 1 μM carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP,
ab120081
,
Abcam
), and 1 μM rotenone (557368, Sigma Aldrich)/1 μM antimycin a (
A8674
,
Sigma Aldrich
). Individual
(12)
Inflammation-induced epigenetic imprinting regulates intestinal stem cells
Cell Stem Cell
October 3, 2024
Dongchang Zhao, Visweswaran Ravikumar, Tyler J. Leach, Daniel Kraushaar, Emma Lauder, Lu Li, Yaping Sun, Katherine Oravecz-Wilson, Evan T. Keller, Fengju Chen,
et al.
Express Enzyme,
Thermo Fisher
,Cat#
12604013
Oligomycin A,MilliporeSigma,Cat# 75351-5mg
FCCP
,
Abcam
,Cat#
ab120081
Antimycin,
MilliporeSigma
,Cat#
A8674
-25mg
Rotenone
,
Tocris
,Cat#
3616
Seahorse XF base medium,Agilent
(13)
Alternative splicing expands the clinical spectrum of NDUFS6-related mitochondrial disorders
Genetics in medicine : official journal of the American College of Medical Genetics
June 1, 2024
Armirola-Ricaurte C, Zonnekein N, Koutsis G, Amor-Barris S, Pelayo-Negro AL, Atkinson D, Efthymiou S, Turchetti V, Dinopoulos A, Garcia A,
et al
.
incubated cells from healthy individuals with 20 nM carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (
ab120081
,
Abcam
) for 5 minutes at 37 °C to depolarize the
mitochondrial membrane
before
staining
. After
staining
,
(14)
Interleukin 21 Drives a Hypermetabolic State and CD4+ T-Cell–Associated Pathogenicity in Chronic Intestinal Inflammation
Gastroenterology
May 1, 2024
Adebowale O. Bamidele, Shravan K. Mishra, Guilherme Piovezani Piovezani Ramos, Petra Hirsova, Emily E. Klatt, Leena M. Abdelrahman, Mary R. Sagstetter, Heidi M. Davidson, Patrick J. Fehrenbach, Lucía Valenzuela-Pérez,
et al.
,
Thermo Fisher Scientific
,Cat#
M7512
ER-Tracker™ Blue-White DPX,
Thermo Fisher Scientific
,Cat#
E12353
Oligomycin
,
Abcam
,Cat#
ab141829
FCCP
,
Abcam
,Cat#
ab120081
Rotenone
,
Abcam
,Cat#
ab143135
Antimycin A,
Abcam
,Cat#
ab141904
2-deoxyglucose (2-DG),APEXBIO,Cat#B1027 Seahorse
(15)
THC and sperm: Impact on fertilization capability, pre-implantation in vitro development and epigenetic modifications
PLoS One
March 27, 2024
Alexander G. Kuzma-Hunt, Reem Sabry, Ola S. Davis, Vivien B. Truong, Jibran Y. Khokhar, Laura A. Favetta
control group
was treated with 0.132mM of carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) (
Abcam
;
ab120081
)–a potent mitochondrial oxidative phosphorylation uncoupler. After the 6-hour treatment period, 200μL
(16)
G2019S selective LRRK2 kinase inhibitor abrogates mitochondrial DNA damage
NPJ Parkinsons Dis
March 1, 2024
Nicholas Pena, Tara Richbourg, Claudia P. Gonzalez-Hunt, Rui Qi, Paul Wren, Carrolee Barlow, Natalie F. Shanks, Holly J. Carlisle, Laurie H. Sanders
followed by three washes with serum-free medium. Complete medium was used to prepare a 20 µM
FCCP
(
Abcam
,
ab120081
) or DMSO control treatment solution, which was used to treat cells for 3 h. Following treatment, cells
(17)
Thioflavin T indicates mitochondrial membrane potential in mammalian cells
Biophysical Reports
December 13, 2023
Emily Skates, Hadrien Delattre, Zoe Schofield, Munehiro Asally, Orkun S. Soyer
was stored at –20°C in the dark. Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP,
Abcam
ab120081
) was dissolved in DMSO to make a 1 mM stock and stored at –20°C in the dark. Ascorbic acid (
Sigma Aldrich
,
(18)
N-acetylneuraminate pyruvate lyase controls sialylation of muscle glycoproteins essential for muscle regeneration and function
Science advances
June 30, 2023
Da Silva A, Dort J, Orfi Z, Pan X, Huang S, Kho I, Heckel E, Muscarnera G, van Vliet PP, Sturiale L,
et al
.
(
Sigma-Aldrich
, catalog no.
O4876
), 400 nM carbonyl cyanide-p-trifluoromethoxyphenyl-hydrazon (
Abcam
, catalog no.
ab120081
), and 10 nM sodium pyruvate (
Gibco
, catalog no.
11360070
), followed by 1 μM antimycin A (
Sigma-Aldrich
,
(19)
Organelle interactions compartmentalize hepatic fatty acid trafficking and metabolism
Cell reports
May 30, 2023
Najt CP, Adhikari S, Heden TD, Cui W, Gansemer ER, Rauckhorst AJ, Markowski TW, Higgins L, Kerr EW, Boyum MD,
et al
.
Grade",
Promega
,
V5280
MitoTracker Deep Red,
Invitrogen
,
M22426
[14C]oleate,
Perkin Elmer
,
NEC317250UC
FCCP
,
Abcam
,
Ab120081
TMRE,
Abcam
,
Ab113852
Etomoxir,
Abcam
,
Ab254445
[13C]palmitate,Sigma-Aldrich,687871–3.80G Ponceau S,Fisher
(20)
STING-Triggered CNS Inflammation in Human Neurodegenerative Diseases
Biomedicines
May 5, 2023
Ferecskó AS, Smallwood MJ, Moore A, Liddle C, Newcombe J, Holley J, Whatmore J, Gutowski NJ, Eggleton P.
fatty acid oxidation (FAO) activator,
FCCP
(carbonyl cyanide-4-(trifluormethoxy) phenylhydrazone,
Abcam
—
ab120081
), was subsequently supplemented into the wells as a positive control, or 1 μL (2.5 μM—final concentration)
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FCCP, mitochondrial oxidative phosphorylation uncoupler from Abcam
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