Immunofluorescence analysis of PSPH was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PSPH Polyclonal Antibody (Product # PA5-22003) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green).Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing predominant cytosolic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Western Blot using PSPH Polyclonal Antibody (Product # PA5-22003). Sample (30 µg of whole cell lysate). A: A431. 12% SDS PAGE. PSPH Polyclonal Antibody (Product # PA5-22003) diluted at 1:1,000.