Immunohistochemistry analysis of VMAT2 was performed on human H1 huES teratoma tissue. To expose target proteins, antigen retrieval was performed using a pressure cooker in 10mM citric acid (pH 6.0). Following antigen retrieval, endogenous peroxidases were blocked with 2.5% hydrogen peroxide. Tissue slide was successively blocked with Avidin D, biotin and protein blocking buffer. Tissues were probed with a VMAT2 polyclonal antibody (Product # PA5-22864) diluted 1:100 overnight at 4°C. After washing tissue slide was incubated with biotinylated anti-rabbit IgG at 37°C for 30 min. After washing tissue slide was incubated at 37°C for 30 min with an HRP kit to conjugate biotinylated anti-rabbit IgG with biotinylated HRP/Avidin. DAB staining for development was performed for 5 min and tissue imaged. Data courtesy of the Antibody Data Exchange Program.