Fig 1: Gene expression signatures of GC PDOs in response to chemotherapeutics(A) Heatmap and (B) volcano plot analysis showing the most significantly differential genes in 5-FU-sensitive organoids (n = 13) compared to organoids with 5-FU resistance (n = 4). The colored bar represents the log2-transformed values. Individual genes are denoted by gene name. Differentially expressed genes (p < 0.05, log2 (fold change) > 1) of 5-FU-sensitive organoids and 5-FU-resistant organoids are indicated in red or blue, respectively.(C) KOBAS of the differential gene expression profiles of 5-FU-sensitive organoids compared to 5-FU-resistant organoids.(D and E) Heatmaps and volcano plot analysis showing the most significantly differential enriched genes in oxaliplatin-sensitive organoids (n = 11) compared to organoids with oxaliplatin resistance (n = 6).(F) KOBAS of the differential gene expression profiles of oxaliplatin-sensitive organoids compared to oxaliplatin-resistant organoids.(G) Knockout of 5-FU-sensitive gene MSMB and S1PR4 in G27T and G72T organoids (n = 3).(H) Knockout of 5-FU-resistant gene FKBP10 in G9T organoids (n = 3).(I) Knockout of oxaliplatin-sensitive gene MYO1A in G27T and G72T organoids (n = 3).(J) Knockout of oxaliplatin-resistant gene NDUFA4L2 in G9T organoids (n = 3).