Fig 2: Upregulation of RPL6 in lung cancer. (A) Immunohistochemistry staining was performed to check RPL6 expression in lung cancer and paracancerous tissues. (B) Western blotting of RPL6 protein level in the 3 lung cancer cell lines. Bar, 100 µm. RPL6, ribosomal protein L6.

Fig 3: Knockdown of RPL6 inhibited cell migration. (A) Transwell results of H1299 and H1975 cells transfected with siCtrl, siRPL6#1, and siRPL6#2. The migrated cells were stained with 0.05% crystal violet. (B) Western blotting results of proteins related to migration in H1299 and H1975 cells transfected with siCtrl, siRPL6#1, and siRPL6#2. **, P<0.01; ***, P<0.001. Bar, 100 µm. RPL6, ribosomal protein L6.

Fig 4: Knockdown of RPL6 inhibited cell proliferation. (A) CCK-8 results showing the viability of H1299 cells transfected with siCtrl, siRPL6#1, and siRPL6#2. (B) CCK-8 results showing the viability of H1975 cells transfected with siCtrl, siRPL6#1, and siRPL6#2. (C) Colony formation results of H1299 and H1975 cells transfected with siCtrl, siRPL6#1, and siRPL6#2. The cells were stained with 0.1% crystal violet. **, P<0.01; ***, P<0.001. Bar, 1 cm. RPL6, ribosomal protein L6.

Fig 5: RPL6 knockdown in H1299 and H1975 cells. (A) RT-qPCR results of RPL6 mRNA levels in H1299 and H1975 cells transfected with siRPL6. (B) Western blotting results of RPL6 protein levels in H1299 and H1275 cells transfected with siRPL6. The loading control was GAPDH. ***, P<0.001. RPL6, ribosomal protein L6.