Fig 2: (a) ELISA of Pax6 eluted using affinity chromatography (Heparin agarose) in the 8 hpf zebrafish embryos (100 embryos/mL) with high immunoreactivity in the first elution and it was used for further purification. All the values presented are the means ± SD of triplicate assays. (b) ELISA of Pax6 eluted using DEAE cellulose fractions in the 8 hpf zebrafish embryos with high immunoreactivity in Fraction D3 and it was used for further purification. All the values presented are the means ± SD of triplicate assays. (c) ELISA of Pax6 eluted using Sephadex G-75 purification in the 8 hpf zebrafish embryos with high immunoreactivity in Elution G6 and it was used for further assays. All the values are presented are the means ± SD of triplicate assays.

Fig 3: Standard graph of ELISA using serially diluted Pax6 as standard protein.

Fig 4: ELISA absorption values of Pax6 expression in the zebrafish embryos during its various stages of development. Results are expressed as mean ± SD (n = 3).