Fig 2: Hsp47 interacting with FKBP10 enhanced the proliferation phenotype. a Interaction between FKBP10 and Hsp47 in glioma cells was detected by immunoprecipitation-Western blot assay. b Cellular localization of FKBP10 and Hsp47 was detected by immunofluorescence staining. DAPI was used to stain nuclei (blue). Scale bar = 30 μM. c, d The expression level of PCNA mRNA, PCNA protein, AKT, p-AKT, CREB, p-CREB and cell proliferation in Hsp47 parental, scramble, siRNAs groups of U118MG and LN229 cells. e Cell proliferation tests in Hsp47 parental, scramble, siRNAs groups of U118MG and LN229 cells by CCK-8 assays. f Representative images of colony formation assays in Hsp47 parental, scramble, siRNAs groups of U118MG and LN229 cells. Statistical results of colony number. The data are represented as means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

Fig 3: Expression level of FKBP10 related molecules in subcutaneous xenografts and glioma tissues. a Representative immunostaining images of FKBP10, p-AKT (Ser473), p-CREB (Ser133) and PCNA in FKBP10 parental and shRNA subcutaneous xenografts. Scale bar = 50 μm. b Representative immunostaining images of Hsp47, p-CREB (Ser133) and PCNA in grade 2, 3 and 4 gliomas. Scale bar = 25 μm. c Correlation analysis between FKBP10 expression and Hsp47 expression. d, e Expression of p-CREB (Ser133) and PCNA in high and low FKBP10 gliomas. f–h Survival analysis of Hsp47, p-CREB (Ser133) and PCNA overexpression in grade 4 glioma. ***P < 0.001

Fig 4: Immunohistochemical staining of HSP47 in SCC (n=30), CIN (n=10), and normal cervical tissue (n=20) by tissue microarray. (A) Negative staining in normal cervical tissue; (B) a weakly stained tumor lesion; (C) a strongly stained tumor lesion; and (D) a moderately stained tumor lesion. (E) Quantification of HSP47 expression. The expression of HSP47 was upregulated in cervical SCC specimens compared with CIN and normal cervical tissue (P<0.0001).

Fig 5: HSP47 mRNA and protein expression levels were suppressed by si-HSP47 transfection in CaSKi and HeLa cells. (A) Expression of HSP47 mRNA as revealed by real-time qRT-PCR. *P<0.0083. (B) Expression of HSP47 protein as revealed by western blot analysis. GAPDH was used as a loading control.