Oct-3/4 (C-10) Antibody sc-5279 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemOct-3/4 (C-10) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-5279
NCBI Gene AliasesOtf-4, OTF3, OTF-3, OCT4, OTF4, OCT3, Oct3/4, Otf3-rs7, Otf3, Otf-3, Oct-3, Oct-4, Otf4, NF-A3, Oct-3/4, Oct3, Oct4, Otf3g
Antibody TypePrimary Antibodies
Quantity200 µg/ml
CloneC-10
ApplicationsWB, IP, IF, IHC(P), FCM, ELISA
Reactivitymouse, rat, human
Conjugate/TagUnconjugated
FormatPurified
ClonalityMonoclonal
Hostmouse
NCBI Full Gene NamePOU domain, class 5, transcription factor 1, POU class 5 homeobox 1
TargetOct-3/4
IsotypeIgG2b

Questions and Answers

What species does this Oct-3/4 antibody work with in experiments?

The Oct-3/4 (C-10) antibody is reactive with mouse, human (Homo sapiens), and rat species, making it suitable for a variety of applications in different model organisms.

Has this antibody been used in any specific applications related to stem cell research?

Yes, the Oct-3/4 antibody has been shown to work in applications such as immunocytochemistry and flow cytometry, as evidenced by its use in studies that assess pluripotency markers in human induced pluripotent stem cells (iPSCs) and mouse embryonic stem cells.

Can this antibody be used for Western blotting, and what are some findings from studies that utilized it?

The Oct-3/4 antibody has been successfully used in Western blotting. For instance, it was employed in research to analyze the expression of pluripotency markers during differentiation processes in embryonic stem cells, providing insights into the molecular mechanisms governing stem cell fate.

What is the significance of using this antibody in studies of cancer stem cells?

The Oct-3/4 antibody has been utilized to investigate cancer stemness in various studies, including those examining the expression of stemness markers in hepatocellular carcinoma cells. Its application helps in understanding the role of stem cell-like properties in cancer progression and treatment resistance.

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Generation and characterization of two human induced pluripotent stem cell lines from myotonic dystrophy type 1 patientsStem Cell ResearchAugust 1, 2026Pooja Darji, Wenqiang Liu, Wenshu Zeng, Jade T. Chao, Paul D. Pang, Matthew T. Wheeler, Marco Perez, Joseph C. Wu(142951–1-AP),AB_1607719 Undifferentiated iPSC state marker,Mouse IgG2bκ Anti-Oct-3/4,1:200,Santa Cruz Biotechnology (Sc-5279),AB_628051 Undifferentiated iPSC state marker,Mouse IgG1κ Anti-Sox2,1:200,Santa Cruz Biotechnology

(2) Generation of two induced pluripotent stem cell lines from patients carrying heterozygous PTEN mutations associated with PTEN Hamartoma Tumor SyndromeStem Cell ResearchAugust 1, 2026Kristina Zdantsevich, Renke Tan, Dong Li, Parker Walther, Uri Ladabaum, Joseph C. WuiPSC State Markers: SOX2, OCT3/4, NANOG",Mouse IgG2bκ anti-Oct-3/4,1:100,Santa Cruz Biotechnology Cat #sc-5279,RRID:AB_628051 "Undifferentiated iPSC State Markers: SOX2, OCT3/4, NANOG",Rabbit anti-NANOG,1:100,Proteintech

(3) Generation of two induced pluripotent stem cell lines from hypertrophic cardiomyopathy patients carrying MYBPC3 mutationsStem Cell ResearchAugust 1, 2026Catherine A. Wu, Matthew A. Wu, Shane R. Zhao, Junyi Sun, Amira G. Flores-Banuelos, Parker Walther, Matthew Wheeler, Nazish Sayed#sc-365823,RRID: AB_10842165 Pluripotency marker,Mouse IgG2bκ anti-Oct-3/4,1:200,Santa Cruz Biotechnology Cat #sc-5279,RRID: AB_628051 Pluripotency marker,Rabbit anti-NANOG,1:200,Proteintech Cat #142951–1-AP,RRID: AB_1607719 Ectoderm

(4) Generation of an induced pluripotent stem cell line from a patient with Loeys–Dietz syndromeStem Cell ResearchAugust 1, 2026Lu Liu, Amit Manhas, Chikage Noishiki, David Wu, Dipti Tripathi, Naima Turbes, Karim Sallam, Jason T. Lee, Nazish Sayed142951–1-AP,",AB_1607719 Pluripotency Markers,Mouse IgG2bκ Anti-OCT-3/4,1:200,"Santa Cruz Biotechnology Cat# sc-5279,",AB_628051 Pluripotency Markers,Mouse IgG1κ Anti-SOX2,1:200,"Santa Cruz Biotechnology Cat# sc-365823,",AB_10842165 Ectoderm

(5) Pasta, a Versatile Transcriptomic Clock, Maps the Chemical and Genetic Determinants of Aging and RejuvenationAdv Sci (Weinh)July 27, 2026Jérôme Salignon, Maria Tsiokou, Patricia Marqués, Enriqueta Rodríguez-Diaz, Hazel Ang, Federico Pietrocola, Christian G. RiedelWaf1/Cip1/CDKN1A p21 (#sc‐6246, Santa Cruz Biotechnology), LaminB1 (12987‐1‐AP, Proteintech) and Oct3/4 (sc‐5279, Santa Cruz Biotechnology). Equal protein loading was monitored with GAPDH (#2118, Cell Signaling) and

(6) Integrative analysis of drug-gene signatures in human pluripotent stem cells reveals prazosin as a novel SQSTM1 regulator for ALS therapeuticsStem Cell ReportsJuly 14, 2026Florine Roussange, Jacqueline Gide, Johana Tournois, Michel Cailleret, Anne Boland, Christophe Battail, Jean-François Deleuze, Hélène Polvèche, Didier Auboeuf, Knut Brockmann, et al.monoclonal anti-LAMP2,Abcam,Cat# ab25631; RRID: AB_470709 Mouse monoclonal anti-Oct3/4,Santa Cruz,"Cat# sc-5279; SantaCruz, RRID: AB_628051" Rabbit monoclonal anti-NANOG,Cell Signaling Technology,Cat# 4903; RRID:

(7) PCBP1 orchestrates amino acid metabolism burst during the naïve-to-primed pluripotency transitionStem Cell ReportsJuly 14, 2026Evgeny I. Bakhmet, Evgeniy V. Potapenko, Oleg Y. Shuvalov, Arseniy A. Lobov, Egor A. Repkin, Daria V. Kriger, Nadezhda E. Vorobyeva, Alexey N. Korablev, Anna S. Zinovyeva, Andrey A. Kuzmin, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Pcbp1,Abcam,Cat# ab74793; RRID: AB_1281060 Oct4,Santa Cruz,Cat# sc-5279 (C-10); RRID: AB_628051 Nanog,Bethyl,Cat# A300-397A; RRID: AB_386108 Oct6,Abcam,Cat# ab272925; RRID:

(8) Decoding stage-specific functions of PRC2 in early embryogenesis uncovers roles in preimplantation development and primordial germ cell fateNat Cell BiolJuly 10, 2026Chengjie Zhou, Meng Wang, Zhiyuan Chen, Yi Zhanganti-H3K27me3 (1:200, Active Motif, 61017), anti-V5 (1:200, Invitrogen, R960–25), anti-OCT4 (1:200, Santa Cruz, sc-5279), anti-GATA4 (1:200, R&D Systems, MAB2606-SP), anti-NANOG (1:200, Abcam, ab80892), anti-CDX2 (1:500,

(9) Opposing function of AEBP2 isoforms fine-tune PRC2 catalytic activityNucleic Acids ResJuly 9, 2026Yingying Li, Cheolan Kwon, Hanbyeol Kim, Chul-Hwan LeeCell Signaling Technology), NANOG (1:2000, #4903S, #8, Cell Signaling Technology), and OCT4 (1:1000, #sc-5279, #G0121, Santa Cruz Biotechnology), EZH2-K514me3 [23, 24]. Lentiviral production and delivery AEBP2

(10) Kat5 cKO mouse replicates biological domain signatures associated with Alzheimer's diseaseAlzheimers DementJune 30, 2026Greg A Cary, Jessica E. Young, Shannon E. Rose, Harald Frankowski, Heather Wilkins, Sai Sruthi Amirtha Ganesh, Julia Draper, Martin Darvas, Mark Bothwell, Suman Jayadev, et al.(Cell Signaling Technology, Catalog No.: 4903) at 1:400 and OCT3/4 (Santa Cruz Biotechnology, Catalog No.: sc‐5279) at 1:100. Alexa Fluor secondary antibodies used were Alexa Fluor 594 goat anti‐rabbit IgG (Thermo Fisher Scientific

(11) Efficient Derivation and Transcriptional Characterization of Mouse Extra-Embryonic Endoderm Stem Cell Lines Generated by Somatic Cell Nuclear TransferStem Cells IntJune 24, 2026Shuaipeng Li, Shu Wei, Guomeng Li, Mei Hu, Jiangwei Lin, Wandong BaoSanta Cruz Biotechnology were against Gata4 (cat. # SC‐1237), Dab2 (cat. # SC‐13982), Oct4 (cat. # SC‐5279), Nanog (cat. # SC‐376915), and Cdx2 (cat. # SC‐166830). Primary antibodies from R&D Systems were against

(12) Microglia derived from human induced pluripotent stem cells are regulated by osteopontin, an endogenous extracellular matrix protein maintaining immune homeostasisFrontiers in NeuroscienceJune 24, 2026Daniel Navin Olschewski, Anna Maria Koenig, Helena Mira Nerone, Elena Gross, Tomo Šarić, Sabine Ulrike Vay, Michael Schroeter, Gereon R. Fink, Maria Adele Rueger1:100, cat# sc-21706, RRID:AB_628386, Santa Cruz Biotechnologies), OCT3/4 (goat, dilution 1:200, cat# sc-5279, RRID:AB_628051, Santa Cruz Biotechnologies), NANOG (rabbit, dilution 1:200, cat# 4903-S, RRID:AB_10559205,

(13) Protocol for minimizing necrotic core formation in iPSC-derived cortical organoidsSTAR ProtocJune 19, 2026Md Mahfuz Al Mamun, Sundari ChettyREAGENT or RESOURCES,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Oct3/4 (1:100),Santa Cruz,sc-5279 (RRID:AB_628051) Nanog (1:200),Cell Signaling,3580 (RRID:AB_2150399) TRA-1-60 (1:200),Cell Signaling,4746

(14) Spatial architecture of autism pathogenesis reveals mosaic structural disarray during early developmentNature CommunicationsJune 13, 2026Li Lin, Tzuen Yih Saw, Nigel Chou, Jie Lin Jolene Goh, Jing Eugene Kwa, Wan Kee Chock, Vipul Singhal, Zheng Li, Mike J. Huang, Huck Hui Ng, et al.also expressed pluripotency markers, SOX2 (Abcam ab92494, 1:500), OCT3/4 (Santa Cruz Biotechnology sc-5279, 1:500) and NANOG (R&D systems, AF1997, 1:500). Generation of cortical organoids Cortical organoids

(15) Generation of Self‐Organizing Macrovascular Constructs by Bioprinting Human iPSC‐Derived Mesodermal Progenitor CellsAdvanced ScienceJune 9, 2026Leyla E. Dogan, Nathaly A. Chicaiza‐Cabezas, Florian Kleefeldt, Philipp Wörsdörfer, Jürgen Groll, Süleyman Ergünat 4°C with primary antibodies diluted in blocking buffer. The following antibodies were used: OCT4 (sc‐5279, Santa Cruz Biotech, Dallas, TX, USA), SOX2 (MAB2018, Santa Cruz Biotech), Brachyury/T (AF2085, Santa

(16) Novel feeder-cell-free 3C system promotes the transition from primed to formative pluripotency, self-renewal, and germline differentiation in rabbit embryonic stem cells in vitroFront Cell Dev BiolJune 8, 2026Xiao Chen, Zhihui Liu, Qing Li, Rahman Ullah, Yuan Gao, Xiyue Wang, Lanjun Liu, Fuliang DuA4306, Abclonal; 1:200), goat anti-TFAP2C (cat. no. AF5059, R&D; 1:200), mouse anti-POU5F1 (cat. no. Sc5279, Santa Cruz; 1:50), and rabbit anti-OTX2 (cat. no. A4351, Abclonal; 1:200). After three washes with

(17) Replication stress primes a trophectoderm fate in embryonic stem cellsCell Death DiscoveryJune 2, 2026Andrea Gnocchi, Christelle El Kai, Cristina Castellan, Lucia Santorelli, Negar Arghavanifard, Angela Bachi, Vincenzo Costanzo(Santa Cruz, sc8408), anti-GAPDH (Sigma-Aldrich, G8795), anti-EOMES (Abcam, ab233345), anti-OCT4 (Santa Cruz, sc-5279), anti-c-JUN (Cell signaling, 9165), anti-p-c-JUN (T91) (Abcam, ab79756), anti-γH2AX (Millipore, 05-636),

(18) Fibroblast Growth Factor 2-Immobilized Biointerfaces Drive Exogenous Transforming Growth Factor β1-Independent Chondrogenesis of Human Mesenchymal Stem Cell and Ectopic Cartilage Tissue FormationBiomaterials ResearchJune 1, 2026Ji Hoon Jeong, Jae Hong Park, Myung Jin Ban, Yeongrok Lee, Hyeongtae Kim, Yura Kim, Gahyun Kim, Sai-Won Kwon, Ju Hun Lee, Sang-Heon Kim, et al.diluted in 1% (w/v) BSA for 1 h at room temperature. The following primary antibodies were used: Oct3/4 (sc-5279, Santa Cruz, Dallas, TX, USA), collagen type I (1:200, ab34710, Abcam, Cambridge, UK), collagen type

(19) Induced pluripotent stem cells from a transgenic minipig model of Huntington's disease reveal early metabolic changesDisease Models & MechanismsJune 1, 2026Irena Rysankova, David Sekac, Hana Hansikova, Katerina Vodickova Kepkova, Petr Vodicka, Michaela Vaskovicova, Marie Altmanova, Stefan Juhas, Jana Juhasova, Eliska Taborska, et al.and then incubated overnight at 4°C with primary antibodies (anti-OCT3/4, Santa Cruz Biotechnology, sc5279, 1:50; anti-NANOG, Cell Signaling Technology, 4903, 1:50; anti-SOX2, Cell Signaling Technology, 3579,

(20) STOML2 Maintains Pluripotency and Cell Cycle Integrity in Human Pluripotent Stem Cells via PHB–ERK SignalingInternational Journal of Stem CellsMay 30, 2026Yena Song, Bomi Kim, Ahyeon Kim, Dongyue No, Jeong Bin Lee, Hyun Kyu Kim, Jae Sang Oh, Sung-Hwan Moon, Dong-yun Shin, Youngsok Choi, et al.used for Western blotting: anti-β-actin (Santa Cruz Biotechnology, sc-47778), anti-OCT3/4 (Santa Cruz, sc-5279; and Cell Signaling Technology, #2890S), anti-NANOG (Cell Signaling Technology, #4893S), anti-STOML2

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6/17/2020Good Santa Cruz Antibody for OCT4 Western Blot Wei XuI would recommend this antibody for OCT4 western blot experiments.
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9/17/2018Oct-3/4 Antibody for Western Blot Lucy LeBlancThis antibody was used in a study of embryonic stem cell differentiation. Thus, we used lentiviral shRNA knockdown of Oct4 as a control for loss of self-renewal. We chose this product as it was ... Read Review

Oct-3/4 (C-10) Antibody

Oct-3/4 (C-10) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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Oct-3/4 (C-10) Antibody from Santa Cruz Biotechnology, Inc.

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