Good Santa Cruz Antibody for OCT4 Western Blot

June 17, 2020

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Oct-3/4 Antibody

Catalog Number:

sc-5279

Clone Number:

C-10

I use this antibody to detect OCT4 by western blot. It works super well.

Experimental Design and Results Summary

Applications

Western Blot

Sample

H9 cell line

Primary Incubation

1;200, 4 degree, overnight

Blocking Agent

5% milk

Secondary Incubation

1:10000, room temperature, 1 hour

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

With clean background, OCT4 bands were detected at the correct molecular weight. The signal was strong and specific.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong and specific signal

The Bad

None

The Bottom Line

I would recommend this antibody for OCT4 western blot experiments.

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