Tom20 (F-10) Antibody sc-17764 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemTom20 (F-10) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-17764
Antibody TypePrimary Antibodies
NCBI Gene AliasesMAS20, 1810060K07Rik, mKIAA0016, Gm19268, MOM19, TOM20
ApplicationsWB, IP, IF, IHC(P), ELISA
Reactivitymouse, rat, human
CloneF-10
Quantity200 µg/ml
FormatPurified
Hostmouse
Conjugate/TagUnconjugated
ClonalityMonoclonal
NCBI Full Gene Nametranslocase of outer mitochondrial membrane 20
TargetTom20
IsotypeIgG2a

Questions and Answers

What species does this antibody react with, and how has it been utilized in research?

The Tom20 (F-10) antibody reacts with mouse, human, and rat species. It has been utilized in various applications, including immunofluorescence assays, where it was used to detect TOM20 in cultured cells, as demonstrated in studies such as and .

Can this antibody be used for Western blotting, and what are some examples of its application in scientific studies?

Yes, the Tom20 antibody has been successfully used in Western blotting. For instance, it was employed to analyze mitochondrial protein levels in studies investigating mitochondrial dysfunction and stress responses, as seen in and .

How does the Tom20 antibody contribute to understanding mitochondrial dynamics in cellular stress conditions?

The Tom20 antibody is crucial for studying mitochondrial dynamics, particularly in the context of cellular stress. It has been used to assess mitochondrial morphology and function in various experimental setups, including those examining the effects of replication stress on mitochondrial homeostasis, as reported in .

What is the recommended dilution for using this antibody in immunofluorescence assays?

The recommended dilution for the Tom20 antibody in immunofluorescence assays is typically around 1:500, as indicated in studies like and .

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) 3D matrix stiffness drives energy metabolism to orchestrate stem cell osteogenesis via microtubule acetylation and mitochondrial dynamicsBioactive MaterialsNovember 1, 2026Zhijie Yang, Jing Na, Yu Liu, Gan Yue, Fei Song, Chiyu Li, Qiusheng Shi, Yubo Fan, Lisha Zhengtransported towards the centre of cells. 4.9 Super-resolution imaging Cells were stained with Tom20 (1:200, sc-17764, Santa Cruz Biotechnology) in 25 μm sections as above. Then cells were incubated with the secondary

(2) Metabolic fatty acid substrates enhance the structural and mechanical performance of human-induced pluripotent stem cell-derived cardiomyocytesCell Mol Life SciAugust 6, 2026Mahmoud Gaballah, Kirsi Penttinen, Chandra Prajapati, Katriina Aalto-Setälä(Invitrogen; 700505). The second group was incubated with mouse monoclonal Tom20 antibody (Santa Cruz; sc-17764) and rabbit anti-sarcomeric α-Actinin polyclonal antibody (Thermo Scientific; BS-10367R). The third

(3) Mitochondrial DNA mutations drive tumor heterogeneity in papillary thyroid carcinomaiScienceJuly 17, 2026Han Sai Lee, Jinsun Lim, Jin-Hyung Heo, Hak Hoon Jun, Young Shin Songupregulated in thyroid cells upon mitochondrial dysfunction. Immunohistochemical staining TOM20 (sc-17764; Santa Cruz Biotechnology, Dallas, TX, USA) immunohistochemical staining was performed on formalin-fixed, paraffin-embedded

(4) TBX20 promotes doxorubicin resistance in breast cancer cells through enhanced ABCC1 expression and inhibition of mitophagyPLoS OneJuly 17, 2026Shuai Nie, Dejian Liu, Jun Hu, Yu Gan, Liangliang Miat 4°C with primary antibodies against LC3 (Abcam, ab192890, 1:300, Rabbit) and TOM20 (Santa Cruz, sc-17764, 1:200, Mouse). The next day, after washing three times with PBST (PBS containing 0.1% Tween-20), cells

(5) Pink1-mediated mitophagy in the endothelium releases proteins encoded by mitochondrial DNA and activates neutrophil responses during inflammationElifeJuly 1, 2026Priyanka Gajwani, Li Wang, Koushik Debnath, Pierina Danos, Young-Mee Kim, Shubhi Srivastava, Zijing Ye, Sarah Krantz, Dong-Mei Wang, Chinnaswamy Tiruppathi, et al.primary antibodies against Pink1 (Cell Signaling Technologies, Cat# 6946 S) and Tomm20 (Santa Cruz, Cat# sc-17764), with AlexaFluor 647 anti-rabbit (Invitrogen, Cat# A32795) and AlexaFluor 488 anti-mouse (Invitrogen,

(6) SARS-CoV-2 ORF3a blocks lysosomal cholesterol egress by disrupting VPS39-regulated NPC2 trafficking and BMP metabolismCell ReportsJune 23, 2026Baley A. Goodson, Valeria Montenegro Montenegro Vazquez, Aliza Doyle, Oralia M. Kolaczkowski, Rui Liu, Jingyue Jia, Morié Ishida, Chunyan Ye, Alison M. Kell, Steven B. Bradfute, et al.9367 STX17,Abcam,RRID:AB_10903821; ab316119 TGN46,BioRad,AHP500GT TOMM20,Santa Cruz,RRID:AB_628381; sc-17764 TOMM20,Cell Signaling Technologies,RRID: AB_2687663: 42406 ULK1,Cell Signaling Technologies,RRID:

(7) Mitochondrial oxidative stress promotes the accumulation of advanced glycation end productsPLoS OneJune 23, 2026Firoz Akhter, Sourav Samanta, Alexandre A. Sosunov, Huawei Wu, Ethan Kim Tieu, Shi Fang Yan, Shirley ShiDu Yan(Cell Signaling Technology, 36406S (D8F6H), 1:5000), VDAC (Calbiochem, 185−197, 1:5000,), TOM20 (Santa Cruz Biotechnology, SC-17764, 1:5000,), and mouse anti-β actin (Sigma, A5441, 1:5000). HRP-conjugated anti-rabbit or anti-mouse IgG

(8) Glucose concentration of neuronal media formulations influences PINK1-dependent mitophagy in human iNeuronsAutophagy RepJune 19, 2026Benjamin O'Callaghan, Daniela Melandri, Darija Soltic, Katharina Cosker, Marc P.M. Soutar, James R. Evans, Hannah Lucas-Clarke, Joe Robin, Sonia Gandhi, Nicol Birsa, et al.Cell Signaling Technology Cat# 62,802, RRID:AB_2799632), mouse anti-TOM20 IgG2a (1:1000; Santa Cruz Biotechnology Cat# sc-17764, RRID:AB_628381), chicken anti-MAP2 IgY (1:5000; Millipore Cat# AB5543, RRID:AB_571049). Cells were

(9) FUNDC1-Associated Regulation of Mitochondrial Function Is Crucial for Preventing Endothelial Injury in HyperglycemiaOxid Med Cell LongevJune 10, 2026Vinothkumar Rethineswaran, Young Joon Hong, Woong Bi Jang, Jaewoo Choi, Hye Ji Lim, Sangmi Park, Eun Ji Lee, Jong Seong Ha, Jisoo Yun, Sang-Mo Kwonof AKT, P70S6K, and 4E‐BP1 (all from Cell Signaling technology); FUNDC1 (#bs‐3227R); Bioss. TOMM20 (#sc‐17764); Beta‐Actin (#sc‐47778) (all from Santa Cruz Biotechnology); p62 (#NBP1‐48320); LA‐3B (#NB100‐2220)

(10) SEC14L2 couples chaperone-mediated autophagy to microtubule stability by targeting Stathmin 1Nature CommunicationsJune 10, 2026Guangyuan Li, Hanfang Xu, Bo Gong, Shunji JiaGM130 (610822, BD Biosciences, 1:2000 for IF), CALX/Calnexin (ab22595, Abcam, 1:1000 for IF), TOMM20 (sc-17764, Santa Cruz, 1:400 for IF), LAMP1 (9091S, Cell Signaling Technology, 1:1000 for IF), LAMP2A (ab125068,

(11) A mitochondria-driven quality control mechanism for peroxisomal membrane proteinsNature CommunicationsJune 10, 2026Sarin Segev-Nakar, Itay Koren#19168), rabbit anti-MUL1 (1:1,000; Proteintech, #16133-1-AP), mouse anti-TOMM20 (1:1,000; Santa Cruz (SC) #17764), rabbit anti-BAG6 (1:1,000; CST #8523), rabbit anti-β-Tubulin (9F3) (1:1,000; CST #2128), rabbit anti-PEX26

(12) Mitofusins are required for specialized mitochondrial morphology and function of rod photoreceptor cellsFront Cell Dev BiolJune 3, 2026Michael Landowski, Ryo Hagimori, Purnima Gogoi, Pawan K. Shahi, Kazuya Oikawa, Vijesh J. Bhute, Gillian J. McLellan, Sakae Ikeda, Ken-Ichi Yamada, Bikash R. Pattnaik, et al.1:50 diluted primary antibody against TOMM20 (Santa Cruz Biotechnology, Hercules, TX, USA; catalog # sc-17764; RRID: AB_62838) at 4 °C with slow shaking. They were rinsed in PBS, and incubated with a 1:250 diluted

(13) Wavelength‐Dependent Photobiomodulation Regulates Macrophage Polarization via Mitochondrial Dynamics and Metabolic ReprogrammingAdvanced ScienceJune 2, 2026Qiusheng Shi, Hao Jia, Jianfei Dong, Linhao Li, Jingqi Cao, Xun Chen, Zhenzhen Jia, Jing Na, Zhijie Yang, Xinyuan Chen, et al.temperature, followed by overnight incubation at 4 °C with a primary antibody against TOM20 (CAS no. sc‐17764, Santa Cruz, 1:200). After additional PBS washes, cells were incubated with a STAR RED–conjugated secondary

(14) In Vitro Sperm–Epididymosomes Interaction Immediately Before Fertilization Changes Sperm Fertility PotentialAndrologyMay 29, 2026Maíra Bianchi Rodrigues Alves, Maria Alice de Almeida, Ana Beatriz Bossois Moura, Laura Gabrielli Haupenthal, Raissa Braido Rangel, Flávio Vieira Meirelles, Juliano Coelho da Silveira, Felipe Perecintemperature for 2 h. Finally, the mitochondrial membrane marker TOM20 (Santa Cruz Biotechnology, USA; cat. SC‐17764) was used as a negative marker to demonstrate the absence of contaminants in the isolated epEVs. For

(15) Porphyromonas gingivalis outer membrane vesicles drive neuroinflammation via TGM2-mediated mitochondria-associated endoplasmic reticulum membranesBMC Oral HealthMay 19, 2026Pengye Zhang, Qingyu Zhu, Kairun Zhang, Shengcai Qi(Thermo Fisher Scientific, United States), and permeabilization, the slices were incubated with anti-TOM20 (5 µg/mL, sc-17764, Santa Cruz Biotechnology, Germany), anti-TGM2 (1:200, 145561, Proteintech, China), anti-NeuN (1:200,

(16) Pharmacological restoration of impaired autophagy in retinal ganglion cells prevents abnormal mitochondrial accumulation and glaucomatous neurodegenerationMolecular NeurodegenerationMay 16, 2026Prabhavathi Maddineni, Balasankara Reddy Kaipa, Bindu Kodati, Karthikeyan Kesavan, Linya Li, J Cameron Millar, Sam Yacoub, Ramesh B. Kasetti, Abbot F. Clark, Gulab S. Zodesources: RBPMS (catalog # 118619, Gene Tex and catalog #PA5-11 9676, Invitrogen), TOMM20 (catalog # sc-17764 from Santa Cruz and catalog # 11802 from Proteintech Inc), CoxIV (catalog # 11242-1, Proteintech), SOD2

(17) α-Synuclein aggregates induce mitochondrial damage and trigger innate immunity to drive neuron–microglia communicationNature CommunicationsMay 15, 2026Ranabir Chakraborty, Stephanie Maya, Veronica Testa, Jara Montero-Muñoz, Takashi Nonaka, Masato Hasegawa, Antonella Consiglio, Chiara Zurzolo30 min at 37 °C). Primary antibodies against the following antigens were used: TOMM20 (Santa Cruz, sc-17764; 1:400), Cytochrome c (BD Pharmigen, 556432, 1:400), dsDNA (Progen, clone AC-30-10, 690014; 1:100),

(18) The role of ATP synthase subunit e (ATP5I) in mediating the metabolic and antiproliferative effects of metformin in cancer cellsElifeMay 15, 2026Guillaume Lefrançois, Emilie Lavallée, Marie-Camille Rowell, Véronique Bourdeau, Farzaneh Mohebali, Thierry Bertomeu, Ana Maria Duman, Maya Nikolova, Mike Tyers, Simon-Pierre Gravel, et al.antibodies were used: Anti-ATP5I (Rabbit, 1:100, #16483-1-AP, Proteintech) and TOMM20 (Mouse, 1:100, #sc-17764, Santa Cruz Biotechnology). The following secondary antibodies were used: anti-mouse AF488 (Goat, 1:2000,

(19) FAM162A Is a Key Regulator of Mitochondrial Structure, Dynamics, and Bioenergetics, Driving Cellular Protection and LongevityAging CellMay 10, 2026Andrea Matamoros, Juan Pablo Soffia, Marcelo Muñoz, Michael Maturana, Ma Andreina Rangel-Ramirez, Alvaro Gonzalez-Ibañez, Gabriela Gómez-Lillo, Cesar Astorga, Lina M. Ruiz, Ramón A. Jorquera, et al.2,ab50843,Abcam,rabbit,1:1000, OPA1,ab42364,Abcam,rabbit,1:1000, OPA1,ab119685,Abcam,mouse,1:1000, OXPHOS,ab110413,Abcam,mouse,1:1000, SDHA,sc‐390381,Santa Cruz Biotechnology,mouse,1:500, TOM‐20,sc‐17764,Santa Cruz Biotechnology,mouse,1:500, Mouse IgG (H + L) HrP,31432,Invitrogen,goat,1:10000, Mouse IgG

(20) TMEM106B C-terminal fragments drive nucleocytoplasmic transport failure and TDP-43 mislocalization in the aging human brainbioRxivMay 4, 2026Kedamawit Tilahun, Janani Parameswaran, Myles Dudley, Daniel Pun, Fuying Ma, Jessee Zhang, Tatiana Bold, Jie Jiang#12987-1-AP), rabbit anti-LAP1 (1:200; Proteintech #21459-1-AP), and mouse anti-TOM20 (1:50; Santa Cruz #sc-17764). Sections were washed and incubated with Alexa Fluor-conjugated secondary antibodies (Invitrogen, 1:500)

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2/7/2020Sensitive Tom20 Antibody Vinay EapenThis a a good antibody to study the degradation of mitochondria by mitophagy.

Tom20 (F-10) Antibody

Tom20 (F-10) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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Tom20 (F-10) Antibody from Santa Cruz Biotechnology, Inc.

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