ACE (2E2) Antibody sc-23908 from Santa Cruz Biotechnology, Inc.

Product Specs
ItemACE (2E2) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price $316.00Supplier Page View Company Product Page
Catalog Numbersc-23908
Antibody TypePrimary Antibodies
NCBI Gene AliasesACE1, CD143, DCP1, DCP
Clone2E2
Quantity200 µg/ml
Reactivitymouse, rat, human
ApplicationsWB, IP, IF, IHC(P)
ClonalityMonoclonal
Conjugate/TagUnconjugated
FormatPurified
Hostmouse
NCBI Full Gene Nameangiotensin I converting enzyme, angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
TargetACE
IsotypeIgG1

Questions and Answers

Can this antibody be used for immunohistochemistry in rat tissues?

Yes, the ACE (2E2) Antibody has been utilized in immunohistochemistry applications, as demonstrated in studies involving rat tissues, where it was used to assess ACE expression levels in various experimental contexts, including those related to hypertension and COVID-19 research.

What specific applications has this antibody been shown to work in?

The ACE (2E2) Antibody has been shown to work in several applications, including Western blotting, immunofluorescence, immunohistochemistry, and immunoprecipitation, as evidenced by its use in multiple scientific studies.

Is this antibody effective for detecting ACE in human cell lines?

Yes, the ACE (2E2) Antibody has been effectively used to detect ACE in human cell lines, such as HEK293 cells, in studies investigating the role of ACE in various physiological and pathological conditions, including its involvement in SARS-CoV-2 entry mechanisms.

What species does this antibody react with, and how has this been validated?

The ACE (2E2) Antibody is reactive with mouse, human, and rat species. Its reactivity has been validated through its use in various studies, including those that employed immunofluorescence microscopy to confirm ACE expression in HEK cell lines transfected with ACE.

Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) High expression of angiotensin-converting enzyme in macrophages exacerbates renal interstitial damage in diabetic miceBiochemical and Biophysical Research CommunicationsFebruary 19, 2026Takayuki Uemura, Keigo Tomita, Masahiro Eriguchi, Kumiko Torisu, Masatoshi Nishimoto, Yushi Uchida, Jorge F. Giani, Kenneth E. Bernstein, Kazuhiko Tsuruya, Toshiaki Nakanomembrane using a Trans-Blot Turbo transfer system (BioRad). Mouse monoclonal anti-ACE (1:1000, 2E2, sc-23908, Santa Cruz Biotechnology, Dallas, TX) and rabbit polyclonal anti-β-actin (1:2000, ab8227, Abcam, Cambridge,

(2) Azelaic Acid Regulates the Renin-Angiotensin System and Improves Colitis Based on Network Pharmacology and ExperimentationACS omegaMay 2, 2023Liao Y, Wu X, Luo W, Chen J, Huang Y, Ma K, Zhang C, Wang J, Yang Y, Deng M, et al.GAPDH mouse mAb (1:2000; cat. no. 60004-1-Ig; ProteinTech Group, China), ACE mouse mAb (1:500; cat. no. sc-23908; Santa Cruz Biotechnology, USA), ACE2 mouse mAb (1:100; cat. no. sc-390851; Santa Cruz Biotechnology,

(3) A tool for nuclear imaging of the SARS-CoV-2 entry receptor: molecular model and preclinical development of ACE2-selective radiopeptidesEJNMMI ResearchApril 19, 2023Darja Beyer, Christian Vaccarin, Xavier Deupi, Ana Katrina Mapanao, Susan Cohrs, Fan Sozzi-Guo, Pascal V. Grundler, Nicholas P. van der Meulen, Jinling Wang, Matthias Tanriver, et al.Santa Cruz Biotechnology, Inc. sc-390851) and mouse anti-ACE (1:50 diluted; Santa Cruz Biotechnology, Inc. sc-23908) in 1% BSA were added, followed by incubation at 4 °C overnight. The cells were rinsed with PBS before

(4) COVID-19: Understanding the impact of anti-hypertensive drugs and hydroxychloroquine on the ACE1 and ACE2 in lung and adipose tissue in SHR and WKY ratsPhysiological reportsFebruary 1, 2023Corrêa BSG, de Barros S, Vaz JB, Peres MA, Uchiyama MK, da Silva AA, Furukawa LNS.were sealed in glass slides and submitted to immunohistochemistry using an anti‐ACE Antibody (2E2: sc‐23908) and anti‐ACE2 antibody (E‐11: sc‐390851) from Santa Cruz and revealed using Dako EnVision® + Dual Link

(5) Heme oxygenase-1 protects against PM2.5 induced endothelial dysfunction through inhibition of HIF1αEnvironmental toxicology and pharmacologyJanuary 1, 2023Xu H, Wen Q, Xu X, Yu D, Liu Z, Zhang C, Zhang X, Ma J, Zhao H, Song L.

(6) Long Chain N3-PUFA Decreases ACE2 Protein Levels and Prevents SARS-CoV-2 Cell EntryInternational journal of molecular sciencesNovember 10, 2022Huang S, Taylor CG, Zahradka P.Hercules, CA, USA) were used to detect ACE2. Mouse anti-ACE1 monoclonal primary antibody (Catalog #: sc-23908, Santa Cruz, Dallas, TX, USA), together with an HRP-conjugated goat anti-mouse secondary antibody (Catalog

(7) Role of Arginase-II in Podocyte Injury under Hypoxic ConditionsBiomoleculesAugust 31, 2022Ren Z, Potenza DM, Ma Y, Ajalbert G, Hoogewijs D, Ming XF, Yang Z.(Waltham, MA, USA); mouse antibody against synaptopodin (sc-515842) and mouse antibody against ACE1 (sc-23908) were from Santa Cruz Biotechnology (Dallas, TX, USA); mouse antibody against tubulin (T5168), mouse

(8) Diminazene aceturate mitigates cardiomyopathy by interfering with renin-angiotensin system in a septic rat modelBMC pharmacology & toxicologyJuly 4, 2022Lu Z, Wu D, Wang Z, Zhang H, Du Y, Wang G.

(9) Angiotensin II receptor 1 controls profibrotic Wnt/β-catenin signalling in experimental autoimmune myocarditisCardiovascular researchJanuary 29, 2022Czepiel M, Diviani D, Jaźwa-Kusior A, Tkacz K, Rolski F, Smolenski RT, Siedlar M, Eriksson U, Kania G, Błyszczuk P.

(10) Shenkang injection improves chronic kidney disease by inhibiting multiple renin-angiotensin system genes by blocking the Wnt/β-catenin signalling pathwayFrontiers in pharmacologyJanuary 1, 2022Wang YN, Liu HJ, Ren LL, Suo P, Zou L, Zhang YM, Yu XY, Zhao YY.States), fibronectin (ab2413, Abcam, United States), E-cadherin (ab76055, Abcam, United States), ACE (sc-23908, Santa Cruz, United States), AT1R (ab124505, Abcam, United States), Wnt1 (ab85060, Abcam, United States),

(11) Identification of genes involved in neuronal cell death and recovery over time in rat axotomy and neurorrhaphy models through RNA sequencingMolecular and cellular neurosciencesJune 1, 2021Choi MJ, Choi JH, Park YS, Park KS, Lee JS.

(12) Role of tubular epithelial arginase-II in renal inflammagingNPJ aging and mechanisms of diseaseMarch 2, 2021Huang J, Liang X, Ladeiras D, Fellay B, Ming XF, Yang Z.(#9234) were purchased from Cell Signaling Technology (Danvers, USA); Mouse antibody against ACE1 (sc-23908), ICAM-1 (sc-8439) and p16 (sc-81156) were from Santa Cruz Biotechnology (Dallas, USA); Rabbit antibody

(13) Renal ischemia-reperfusion leads to hypertension and changes in proximal tubule Na⁺ transport and renin-angiotensin-aldosterone system: Role of NADPH oxidaseLife sciencesFebruary 1, 2021Lima NKS, Farias WRA, Cirilo MAS, Oliveira AG, Farias JS, Aires RS, Muzi-Filho H, Paixão ADO, Vieira LD.

(14) Uncovering the mechanisms of leech and centipede granules in the treatment of diabetes mellitus-induced erectile dysfunction utilising network pharmacologyJournal of ethnopharmacologyJanuary 30, 2021Ma JX, Wang B, Li HS, Yu J, Hu HM, Ding CF, Chen WQ.

(15) Lactation Leads to Modifications in Maternal Renin-Angiotensin System in Later LifeReproductive sciences (Thousand Oaks, Calif.)January 1, 2020La Rosa M, Kechichian T, Olson G, Saade G, Bytautiene Prewit E.

(16) Exposure to Traffic-Generated Pollutants Exacerbates the Expression of Factors Associated with the Pathophysiology of Alzheimer's Disease in Aged C57BL/6 Wild-Type MiceJournal of Alzheimer's disease : JADJanuary 1, 2020Armstrong TD, Suwannasual U, Kennedy CL, Thasma A, Schneider LJ, Phillippi D, Lund AK.#11713), Aβ (1:500, Novus Biologicals, Littleton, CO, #NBP2–13075), ACE (1:1000, Santa Cruz Biotechnology #sc-23908), or BACE1 (1:1000, Santa Cruz Biotechnology #sc-33711SS). The secondary antibodies anti-rabbit Alexa

(17) Genetic disruption of guanylyl cyclase/natriuretic peptide receptor-A upregulates renal (pro) renin receptor expression in Npr1 null mutant micePeptidesApril 1, 2019Periyasamy R, Das S, Pandey KN.utilized in the Western blot analyses in the current work. Protein Description Source ACE Cat# SC-23908 Santa Cruz, Biotechnology (Santa Cruz, CA) Beta-actin Cat # SC-47778HRP Santa Cruz, Biotechnology

(18) Rapeseed protein-derived peptides, LY, RALP, and GHS, modulates key enzymes and intermediate products of renin-angiotensin system pathway in spontaneously hypertensive ratNPJ science of foodJanuary 1, 2019He R, Yang YJ, Wang Z, Xing CR, Yuan J, Wang LF, Udenigwe C, Ju XR.purchased from Solarbio Science and Technology Co., Ltd. (Beijing, China). Primary antibodies against ACE (sc-23908), ACE2 (sc-390851), renin (sc-137252,) and Ang II (sc-74402) as well as secondary antibodies and anti-GAPDH

(19) Prenatal high-salt diet impaired vasodilatation with reprogrammed renin-angiotensin system in offspring ratsJournal of hypertensionDecember 1, 2018Liu Y, Qi L, Wu J, Xu T, Yang C, Chen X, Lv J, Xu Z.

(20) Novel inhibitors of the cellular renin-angiotensin system components, poricoic acids, target Smad3 phosphorylation and Wnt/β-catenin pathway against renal fibrosisBritish journal of pharmacologyJuly 1, 2018Wang M, Chen DQ, Chen L, Cao G, Zhao H, Liu D, Vaziri ND, Guo Y, Zhao YY.