Fig 1: Co-expression of CD16 and MHC-II on γδ T-IELs in porcine jejunum. (A) Representative flow cytometry gating showing the expression of CD16 and MHC-II on gated γδ T-IELs in epithelial-enriched cell fractions from jejunum of a 6-week-old pig. (B) Stacked bar plot of the percentage of CD16+ γδ T-IELs that are MHC-II+ (turquoise) versus MHC-II- (salmon). Data are derived from jejunum of eight 6-week-old pigs. Epithelial-enriched cell fractions were isolated from jejunum and stained for flow cytometry as previously described (67). Detection reagents used for flow cytometry staining are as follows: Fixable Viability Dye eFluor780 (ThermoFisher Scientific 65-0865-14); anti-CD3ε-PE-Cy7 (clone BB23-8E6-8C8; BD 561477); anti-γδTCR (clone PGBL22A; Washington State University PG2032; conjugated to mFluor510 fluorophore by Caprico Biotechnologies); anti-CD16 (clone G7; BioRad MCA1971GA) detected with anti-mouse IgG1 (clone A85-1; BD 740234); MHC-II (clone 2E9/13; BioRad MCA2314GA) detected with anti-mouse IgG2b-BUV496 (clone R12-3; BD 750517). FSC-A (forward-scatter area); FSC-H (forward-scatter height); Ig (immunoglobulin); MHC (major histocompatibility complex); SSC-A (side-scatter area); T-IEL (intraepithelial T lymphocyte); TCR (T cell receptor).