Myeloperoxidase/MPO Antibody AF3667 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMyeloperoxidase/MPO Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF3667
ApplicationsELISA Development,Flow Cytometry,ELISA,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Immunocytochemistry,Functional Assay,Dual RNAscope ISH-IHC Compatible,Immunocytochemistry/ Immunofluorescence,ELISA Capture
Conjugate/TagUnconjugated
Quantity100 ug (also 25 ug)
ReactivityHamster,Human,Mouse,Primate,Rabbit,Rat,Transgenic Mouse,Xenograft
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
HostGoat
TargetMyeloperoxidase/MPO
NCBI Full Gene Namemyeloperoxidase
ClonalityPolyclonal
NCBI Gene AliasesmKIAA4033
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse MPO, Met16-Thr718, Accession # P11247
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human and mouse Myeloperoxidase/MPO in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse EPPO is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this myeloperoxidase antibody be used in immunohistochemistry for studying lung inflammation?

Yes, the Myeloperoxidase/MPO Antibody has been utilized in immunohistochemistry applications, as evidenced by its use in studies examining neutrophil infiltration in various models, including acute respiratory distress syndrome and inflammatory bowel disease, where it helped to visualize neutrophil presence in tissue sections.

Is this antibody effective for detecting myeloperoxidase in mouse models?

The Myeloperoxidase/MPO Antibody has been shown to effectively detect myeloperoxidase in mouse models, as indicated by its application in studies involving mouse pneumonia models and other inflammatory conditions, where it contributed to understanding neutrophil function and inflammation.

What species can this antibody react with, and how does that impact its utility in research?

This antibody is reactive with several species, including humans, mice, rats, non-human primates, and hamsters. This broad reactivity makes it a versatile tool for comparative studies across different animal models and human samples, facilitating research in various fields such as immunology and cancer biology.

Are there any specific applications in which this antibody has been cited in the literature?

Yes, the Myeloperoxidase/MPO Antibody has been cited in multiple studies, including those investigating neutrophil extracellular traps in severe asthma and the role of neutrophils in colorectal cancer inflammation. Its use in these contexts highlights its relevance in studying neutrophil-related pathologies and inflammatory responses.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Urinary UDP-sugars provide actionable prediction of acute kidney injury in cardiac surgery patientsAmerican Journal of Physiology-Heart and Circulatory PhysiologyOctober 1, 2026Christine Légaré, Patrick Caron, Mohammad M. Al-bataineh, Aisha Ford, David R. Emlet, Jessica Deslauriers, Dennis Brown, Chantal Guillemette, Siamak Mohammadi, Sylvie BretonAdditional sections were double-labeled using a goat antibody against myeloperoxidase (MPO) (R&D Systems AF3667; 1:100 dilution) followed by donkey anti-goat antibody conjugated to Cy3, together with a mouse antibody

(2) DVT-primed neutrophils reshape the brain microenvironment to promote brain metastasisbioRxivSeptember 17, 2026Shun Endo, Denys Rujchanarong, Karen Keeran, Kenneth Jeffries, Wei Zhang, Maxwell Bannister, Keita Saeki, Debbie Wei, Andy Tran, Kate Ellison, et al.and 5% bovine serum albumin (BSA); and stained with antibodies to citH3 (#ab281584, Abcam) and MPO (# AF3667, R&D Systems) at 1:200 dilution in blocking buffer at 4 °C overnight. Then, the cells were washed and

(3) Neutrophil Senescence Induced by Tumor-Derived HMGB1 Promotes Hepatocellular Carcinoma Progression through Neutrophil Extracellular Trap-Mediated Suppression of CD8+ T Cell ImmunityCancer CommunicationsSeptember 11, 2026Yaqian Duan, Shan Yu, Xuanyi Wang, Rong Wu, Yuqing Zhao, Xuehua Kong, Shiyu Cao, Dan Xiang, Rui Wu, Jingying Zhou, et al.(11774425001; Roche). Briefly, 96-well plates were precoated with 5 μg/ml anti-MPO capture antibody (AF3667; Bio-techne) overnight at 4 °C. After blocking with 1% bovine serum albumin, samples were incubated

(4) NEAT1 modulates neutrophil functions via glycolysis to restrain mucosal inflammation in ulcerative colitisFrontiers in ImmunologyAugust 19, 2026Fengqin Zhu, Guiyuan Jin, Lihao Shi, Fengxian Dai, Cuimei Ma, Zongjing Hu, Yonghong Yang, Guanjun Dong, Guangxi Zhou20874-1AP; Proteintech), CD4 (1:200; ab133616; Abcam), F4/80 (1:200; ab300421; Abcam), MPO (1:100; AF3667; Biotechne), CitH3 (1: 200; ab5103; Abcam), or Ly6G (1: 200; RB6-8C5; Invitrogen). Samples were then

(5) A Premature Rabbit Kitten Model for Evaluation of Bronchopulmonary Dysplasia Therapies Reproduces Myeloperoxidase-Associated Pathology and Therapeutic ResponsesInt J Mol SciAugust 15, 2026Carlos Dounce, Xigang Jing, Keguo Li, Deron Jones, Tzong-Jin Wu, Adeleye J. Afolayan, Girija Ganesh Konduri, Billy W. Day, Kirkwood A. Pritchard, Stephen Naylor, et al.concentration of 10 mg/mL and filtered through a 0.22 μm syringe filter for injection. Goat anti-MPO antibody (AF3667) from R&D Systems (Minneapolis, MN, USA) was used for immunoblots. Rabbit anti-MPO antibody (EPR17996)

(6) Amiloride mitigates respiratory distress caused by WFDC2 deficiency via inhibiting the epithelial sodium channelNature CommunicationsAugust 12, 2026Jae Won Roh, Soo Kyung Seo, Jiyoung Oh, Se Jin Kim, Ji Won Hong, Jiyeon Ohk, Hyo Sup Shim, Hyung-Ju Cho, Ala Woo, Song Yee Kim, et al.secondary antibodies were used: anti-Neutrophil Elastase (#68672, Abcam; 1:200 dilution), anti-MPO (#AF3667, R&D Systems; 1:200 dilution), anti-Scgb1a1 (#213203, Abcam; 1:200 dilution), Alexa Fluor 488 donkey

(7) Integrative multi-omics analysis identifies VCAN and ESM1 as candidate cell-type-associated biomarkers of NET-related glomerular injury in diabetic kidney diseaseRen FailAugust 6, 2026Yufeng Liang, Chenlun Li, Binsan Huang, Azhen Wang, Meilan Qiu, Juan Lu, Jianxin Wanantigen retrieval, and incubated with primary antibodies against myeloperoxidase (MPO; R&D Systems, AF3667, 1:200), Cit-H3 (Abcam, ab5103, 1:100), Versican (VCAN; ImmunoWay, YT4874, 1:150), and Endothelial cell-specific

(8) Deciphering the regulatory role of ADAM8 in the PDAC tumor microenvironmentCell Oncol (Dordr)August 3, 2026Kimia Zandieh, Lena Cook, Kai Zhao, Constanze Nagl, Yutong Gao, Pietro diFazio, Detlef K. Bartsch, Uta-Maria Bauer, Marion Meixner, Daniela Yildiz, et al.compare neutrophil levels in the mice tissues, anti-Human/Mouse Myeloperoxidase/MPO antibody (1:100; AF3667, R&D Systems, Minneapolis, MN, USA) was utilized. The protocol for staining of macrophages and neutrophils

(9) Impaired NLRP3 inflammasome activation by chitin underlies refractory chromoblastomycosis caused by Fonsecaea pedrosoi muriform cellsFront ImmunolJuly 14, 2026Yao Chen, Zilu Qu, Zhaolan Xie, Xiaowen Wang, Zhongsheng Tong, Luoyao Yang, Xu Zhang, Liuqing Chen, Bilin Dongbinding. Subsequently, sections were incubated overnight at 4°C with a goat anti-h/mMPO antibody (1:80, #AF3667, R&D systems), with a rabbit anti-hCD68 antibody (1:500, #76437, Cell Signaling) or with a rat anti-h/mNLRP3

(10) CHAC2-mediated glutathione metabolic reprogramming drives N1 polarization of bone marrow neutrophils and exacerbates inflammatory comorbiditiesInt J Oral SciJuly 9, 2026Yuting Niu, Yuman Li, Shiyu Sun, Chenyu Deng, Fan He, Yiming Chen, Tiansong Xu, Zhewen Hu, Gai Yang, Leran Li, et al.(ab92517, Abcam), anti-Insulin (ab181547, Abcam), anti-Chac2 (16304-1-AP, Proteintech), and anti-MPO (AF3667, R&D systems). The images were then scanned with ZEISS AXIOSCAN 7 and analyzed with the ImageJ software. Immunofluorescence

(11) MG53 Coordinates Macrophage Polarization and Neuroimmune Coupling to Promote Corneal Nerve Regeneration via the MPEG1-MVP-STAT6 AxisAdvanced science (Weinheim, Baden-Wurttemberg, Germany)July 1, 2026Chen P, Zhang Z, Sakai L, Xu Y, Zhang L, Ye H, Sollenberger W, Ikeda T, Yan Z, Sathish K, et al.β3‐tublin (Thermo Scientific, cat#PA5‐85639), CD31 (BD Biosciences, cat#550274), MPO (R&D Systems, cat#AF3667), CD206 (BioLegend, Alexa Fluor 647, cat#141712), CD80 (Thermo Fisher, cat#16‐0801‐82), CD11b (BioLegend,

(12) Prognostic significance of intratumoral neutrophil extracellular traps in pulmonary pleomorphic carcinoma: comparison with adenocarcinomaBJC RepJune 12, 2026Makoto Yoden, Ryosuke Kaku, Daigo Ishihara, Kohei Shibata, Keiko Ueda, Takuya Shiratori, Yoko Kataoka, Yo Kawaguchi, Jun Hanaoka, Fumihiro Shojiwith primary antibodies against CitH3 (NB100-57135, Novus Biologicals, Centennial, CO, USA) and MPO (AF3667, R&D Systems Minneapolis, MN, USA). After washing, the slides were incubated with Alexa Fluor 647 donkey

(13) Mutation-dependent responses to sleep and exercise in clonal haematopoiesisNatureJune 10, 2026Teresa Gerhardt, Walter Jacob, Lena Gaebel, Merlin Heiser, Christopher Wolfram, Pacific Huynh, Tetsushi Nakao, Bernardo Gindri Gindri Dos Santos, Pamela Toh, Aaron Douglas, et al.(SolA15, Invitrogen), anti-IL1b, anti-IL1R1 (MA5–40958 or MA5–40959, Invitrogen), anti-MPO (1:100; AF3667, Bio-Techne) and anti-histone H3 (1:100; ab5103, Abcam). For intracellular staining, extracellular antibody

(14) Replicative and stress-induced premature senescence distinctively affect the endothelial anticoagulation capacityPLoS OneJune 9, 2026Akiko Katayama, Koji Ikeda, Tomoya Kitani, Ekura Yamazaki, Daisuke Ueno, Fumiaki Ito, Satoaki Matoba[13]. Materials and methods Reagents Anti-human myeloperoxidase (MPO) antibody was obtained from R&D Systems (#AF3667). Antibodies against myc and von Willebrand factor were purchased from MBL life science (#M192-3) and

(15) Neutrophil extracellular traps offer a new therapeutic target for elephant endotheliotropic herpes virus-hemorrhagic disease (EEHV-HD)Communications BiologyMay 30, 2026Lisa M. Abegglen, Aaron Rogers, Gareth Mitchell, C. Bradley Nelson, Madison I. Sanborn, Ryan Kennington, McKenna Rogers, Virginia R. Pearson, Miranda Sharp, Lauren L. Howard, et al.avoiding contamination from the other layers. Cells were stained with goat anti-human/mouse MPO (1:67, AF3667; R&D Systems) and DyLight 649-conjugated anti-goat secondary antibodies. ProLong Glass Antifade Mountant

(16) ICAM1high Neutrophils Sculpt Tumor Evolution and Metastasis through Symbiotic Adhesion and Reverse MigrationCancer ResMay 28, 2026Ling Wu, Zhan Xu, Jun Wang, Fengshuo Liu, Yunfeng Ding, Yang Gao, Siyue Wang, Jun Liu, Hilda L. Chan, Weiguo Wu, et al.RRID: AB_10001164), anti-RFP (Rockland, Cat#600-401-379, RRID: AB_11182807), anti-MPO (R&D System Cat#AF3667; RRID: AB_2250866), anti-ICAM1 (Abcam, Cat# ab222736, RRID: 3697845), anti-Ki67 (ThermoFisher, Cat#14-5698-80;

(17) Neutrophil extracellular traps are found at epithelial barriers in canine sinonasal aspergillosisFront ImmunolMay 1, 2026Pierre Janssen, Constance de Meeûs, Mutien-Marie Garigliany, Cecile Clercx, Thomas Marichal, Coraline Radermecker, Frederic Billenwere coated with anti-human MPO antibody (3,125.103 mg/mL, goat anti-human/mouse MPO, R&D Systems, AF3667) in PBS overnight at 4 °C. The day after, plates were blocked with PBS-Bovine Serum Albumin 1% (BSA,

(18) Lipid asymmetry disruption by XKR8 orchestrates neutrophil extracellular trap formation and inhibits fungal infectionNature immunologyMay 1, 2026Liu W, Ping J, Deng L, Wang K, Xu J, Peng N, Yang M, Tang H, Liu Z, Wang W, et al.dilution) and anti-neutrophil elastase (ab314916, 1:100 dilution) were purchased from Abcam. Anti-MPO (AF3667) was obtained from R&D systems. Antibodies against caspase-3 (9662), caspase-7 (D6H1, 8438), caspase-8

(19) PAF Triggered Pyroptotic NETosis Aggravates Myocardial Ischemia/Reperfusion InjuryAdvanced ScienceMay 1, 2026Jiawei Wu, Shule Zhang, Ruofan Du, Lina Kang, Guodong Zhao, Xue Bao, Haochi Yang, Ziqing Xie, Tianyu He, Huiyong Sun, et al.(ab1791), and anti‐GSDMD antibody (ab209845) were obtained from Abcam (Cambridge, UK). Anti‐MPO antibody (AF3667) were obtained from NOVUS Biologicals (Centennial, CO, USA). Cleaved Gasdermin D (#10137) was obtained

(20) Small Subset, Big Impact: Regulatory Function of γδ T Cells in ArteriogenesisCellsApril 17, 2026Kira-Sofie Wimmer, Carolin Baur, Matthias Kübler, Christoph Arnholdt, Konda Kumaraswami, Franziska Heim, Katharina Elbs, Michael Reha Rohrmoser, Daphne Merkus, Elisabeth Deindland blocked with 10% donkey serum (Abcam), and incubated overnight with anti-myeloperoxidase (MPO, AF3667, R&D Systems, Minneapolis, MN, USA; 1:100). Secondary detection was performed with Donkey anti-Goat

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10/18/2022Extremely Reliable and Clean MPO Antibody stephen ChetwyndUsed in neutrophil degranulation assays, where we compare the total MPO in mouse neutrophils to the degranulated MPO after bacterial stimulation.