GLI-2 Antibody AF3635 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemGLI-2 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF3635
ApplicationsIF/ICC,Chromatin Immunoprecipitation,Immunohistochemistry,Immunocytochemistry/Immunofluorescence,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry
Conjugate/TagUnconjugated
HostGoat
Quantity100 ug (also 25 ug)
ReactivityDrosophila,Human,Mouse,Transgenic Mouse,Frog - Xenopus (African Clawed Frog)
NCBI Full Gene NameGLI-Kruppel family member GLI2, GLI family zinc finger 2
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse GLI-2, Met1-Glu416, Accession # NP_001074594
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Gene AliasesPHS2, HPE9, THP1, THP2, CJS
IsotypeIgG
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetGLI-2

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse GLI-2 in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) RAC1 regulates Sonic Hedgehog-medulloblastoma growth via GLI-mediated transcriptionNeuro-OncologyJune 1, 2026Nitish Jangde, Mi-Hye Lee, Luz Ruiz, Isabelle Egan, Anna M Jermakowicz, Rishika Chowdary, Jonathan Chu, Daniel T Wynn, Erik Goka, Marc Lippman, et al.Cell Signaling Technology, RRID: AB_10548197), GLI1 (catalog no. AF3455, R&D Systems, RRID: AB_2247710), GLI2 (catalog no. AF3635, R&D Systems, RRID: AB_2111902), and rabbit IgG (catalog no. 12370, Millipore Sigma). DAPI staining

(2) Proximity labeling proteomics maps radial glial ciliary proteins across the developing telencephalonCell ReportsMay 26, 2026Xiaoliang Liu, Oscar T. Gutierrez, Sabyasachi Baboo, Eva Cai, Gurleen Kaur, Yazan Al-Issa, Jolene K. Diedrich, John R. Yates, Xuecai GeIF),Thermo Fisher Scientific,Cat#: A-11122 RRID: AB_221569 Goat anti-GLI2 (1:200 for IF),R&D Systems,Cat#: AF3635 RRID: AB_2111902 Rabbit anti-GLI3 (1:1000 for WB),Novus Biologicals,Cat#: NBP2-16665 RRID: AB_3263717 Goat

(3) Loss-of-function variants in ciliary genes confer high risk for tetralogy of FallotScience advancesOctober 10, 2025Zhou Y, Jiang T, Gao J, Zang J, Mo X, Yue S, Cui Y, Wang Q, Da M, Xu J, et al.1:1000 dilution), GLI1 (Proteintech, 66905-1-Ig, RRID:AB_2882232, 1:1000 dilution), GLI2 (R&D Systems, AF3635, RRID:AB_2111902, 1:1000 dilution), GATA4 (Santa Cruz Biotechnology, sc-25310, RRID:AB_627667, 1:500

(4) RAC1 Regulates Shh-Medulloblastoma Growth via GLI-Mediated TranscriptionbioRxiv : the preprint server for biologyMay 23, 2025Jangde N, Lee MH, Ruiz L, Egan I, Jermakowicz AM, Wynn D, Goka E, Lippman M, Robbins DJ, Ayad NG.

(5) Proteomic profiling of primary cilia in the developing brain uncovers new regulators of cortical developmentbioRxiv : the preprint server for biologyMay 4, 2025Liu X, Gutierrez OT, Kaur G, Al-Issa Y, Baboo S, Diedrich JK, Cai E, Yates JR 3rd, Ge X.

(6) Morphogen-induced kinase condensates transduce Hh signal by allosterically activating GliSci AdvJanuary 10, 2025Yuhong Han, Mengmeng Zhou, Bing Wang, Jin Jiangmouse anti–α-tubulin (T9026, MilliporeSigma), rabbit anti-histone3 (ab1791, Abcam), goat anti-Gli2 (AF3635, R&D systems), rabbit anti-SUMO1 (4930, Cell Signaling Technology), mouse anti-acetylated tubulin (T7451,

(7) Proximity based proteomics reveals Git1 as a regulator of Smoothened signalingbioRxivJanuary 7, 2025Jingyi Zhang, Gurleen Kaur, Eva Cai, Oscar Torres Gutierrez, Xiaoliang Liu, Sabyasachi Baboo, Jolene K Diedrich, Ju-Fen Zhu, Benjamin R. Myers, John R Yates, et al.(BiCell Scientific, 90413), rabbit anti-IFT88 (Proteintech, 13967–1-AP), Goat anti-Gli2 (R&D Systems, AF3635,), Goat anti-Gli1 (R&D Systems, AF3455), Rabbit anti-Git1 (Novus Biologicals, NBP1–86144), Chicken anti-GFP

(8) Intervertebral disc-intrinsic Hedgehog signaling maintains disc cell phenotypes and prevents disc degeneration through both cell autonomous and non-autonomous mechanismsCellular and Molecular Life SciencesDecember 1, 2024Lei Zhang, Siyuan Hu, Chunmei Xiu, Meng Li, Yixin Zheng, Rui Zhang, Bin Li, Jianquan ChenProteintech) or Gli1 (1:2000, #66905-1-Ig, Proteintech), goat primary antibodies against Gli2 (1:3000, #AF3635, R&D System) or Gli3 (1:2000, #AF3690, R&D System), or rabbit primary antibody against β-actin (1:2000,

(9) CPLANE protein INTU regulates growth and patterning of the mouse lungs through cilia-dependent Hh signalingDevelopmental BiologyNovember 1, 2024Huiqing Zeng, Shimaa Ali, Aswathy Sebastian, Adriana Sophia Ramos-Medero, Istvan Albert, Charlotte Dean, Aimin Liuanti-ARL13B,Tamara Caspary,n/a Rat monoclonal anti-CDH1,Sigma-Aldrich,U3254 Goat polyclonal anti-GLI2,R&D System,AF3635 Goat polyclonal anti-GLI3,R&D System,AF3690 Mouse monoclonal anti-Acta2,Sigma-Aldrich,A2547 Mouse

(10) mTORC1 hampers Hedgehog signaling in Tsc2 deficient cellsLife Science AllianceNovember 1, 2024Lasse Jonsgaard Larsen, Elsebet Østergaard, Lisbeth Birk Møller(1:1,000, ab38686; Abcam), mouse anti-Arl13b (1:1,000, #ab136648; Abcam), goat anti-Gli2 (1:1,000, AF3635; R and D Systems), rabbit anti-TSC2 (1:1,000, D93F12; Cell Signaling). Secondary antibodies used were

(11) PP2A phosphatase regulatory subunit PPP2R3C is a new positive regulator of the hedgehog signaling pathwayCellular signallingNovember 1, 2024Baran B, Derua R, Janssens V, Niewiadomski P.

(12) The Proteasome and Cul3-Dependent Protein Ubiquitination Is Required for Gli Protein-Mediated Activation of Gene Expression in the Hedgehog PathwayCellsSeptember 6, 2024Uśpieński T, Niewiadomski P.Number,Application (Dilution) Primary antibodies,,, Anti-Gli2,"R&D Systems (Minnneapolis, MN, USA)",AF3635,WB (1:1000) Anti-Gli1,"Cell Signaling Technologies (Danvers, MA, USA)",2643,WB (1:1000) Anti-Gli3,"R&D Systems

(13) Ulk4 promotes Shh signaling by regulating Stk36 ciliary localization and Gli2 phosphorylationElifeDecember 14, 2023Mengmeng Zhou, Yuhong Han, Jin Jiangstaining 1:2000), rabbit anti-HA (Abcam; ab9110; staining 1:500), and goat anti-Gli2 (R&D Systems; AF3635; staining 1:1000). In vitro kinase assay In vitro kinase assay was performed by incubating 25 μl of

(14) Ulk4 promotes Shh signaling by regulating Stk36 ciliary localization and Gli2 phosphorylationElifeDecember 14, 2023Mengmeng Zhou, Yuhong Han, Jin Jiangstaining 1:2000), rabbit anti-HA (Abcam; ab9110; staining 1:500), and goat anti-Gli2 (R&D Systems; AF3635; staining 1:1000). In vitro kinase assay In vitro kinase assay was performed by incubating 25 μl of

(15) Increasing Ciliary ARL13B Expression Drives Active and Inhibitor-Resistant Smoothened and GLI into Glioma Primary CiliaCellsSeptember 26, 2023Shi P, Tian J, Mallinger JC, Ling D, Deleyrolle LP, McIntyre JC, Caspary T, Breunig JJ, Sarkisian MR.#T6557 Green fluorescent protein (GFP),Chicken,1:5000,Abcam; Cat #13970 GLI2,Goat,1:500,R&D Systems; Cat #AF3635 Inositol polyphosphate 5-phosphatase (INPP5E),Rabbit,1:1000,Proteintech; Cat #17797-1-AP Intraflagellar

(16) Targeted protein degradation reveals BET bromodomains as the cellular target of Hedgehog pathway inhibitor-1Nature communicationsJuly 1, 2023Bagka M, Choi H, Héritier M, Schwaemmle H, Pasquer QTL, Braun SMG, Scapozza L, Wu Y, Hoogendoorn S.mouse anti-ARL13B (Biolegend, clone N295B/66, lot B323369), 1:500 goat anti-mouse GLI2 (R&D systems, AF3635, lot XUA0320091), 1:500 goat anti-mouse GLI3 (R&D systems, AF3690)) overnight at 4 °C, washed 5 × 5

(17) A Druggable UHRF1/DNMT1/GLI Complex Regulates Sonic Hedgehog-Dependent Tumor GrowthMolecular cancer research : MCRNovember 3, 2022Yang F, Rodriguez-Blanco J, Long J, Swiderska-Syn M, Wynn DT, Li B, Shen C, Nayak A, Ban Y, Sun X, et al.Cell Signaling Technology, RRID:AB_10548197), GLI1 (prepared as described before [39]), GLI2 (Cat# AF3635, R&D Systems, RRID:AB_2111902), rabbit IgG (Cat# 12370, MilliporeSigma), mouse IgG (Cat# sc2025, Santa Cruz Biotechnology

(18) Zic5 stabilizes Gli3 via a non-transcriptional mechanism during retinal developmentCell reportsFebruary 1, 2022Sun J, Yoon J, Lee M, Lee HK, Hwang YS, Daar IO.

(19) Myomegalin regulates Hedgehog pathway by controlling PDE4D at the centrosomeMolecular biology of the cellSeptember 1, 2021Peng H, Zhang J, Ya A, Ma W, Villa S, Sukenik S, Ge X.GFP antibody (A11122, ThermoFisher), mouse anti-acetylated tubulin (T6793, SIGMA), goat anti-Gli2 (AF3635, R&D SYSTEMS), and rabbit anti-pPKA (ab59218, Abcam). Quantification of Phospho-PKA, PDE4D3, and Gli2 The

(20) Ciliary and extraciliary Gpr161 pools repress hedgehog signaling in a tissue-specific mannereLifeAugust 4, 2021Hwang SH, Somatilaka BN, White K, Mukhopadhyay S.immunoblotting as described previously (Wen et al., 2010), using Gli3 (AF3690, R and D, 1 µg/ml), Gli2 (AF3635, R and D, 1 µg/ml), Gli1 (L42B10, Cell Signaling; 1:1000) and α-tubulin (clone DM1A, T6199, Sigma; 1:5000)

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(1)

1/26/2016GLI2 Antibody for Immunofluorescence Ganesh PusapatiI am interested in understanding the mechanism of Hedgehog signal transduction in cultured fibroblast cells. The goal of this experiment is to test if GLI2 protein translocates to nucleus after ... Read Review
(1)

1/20/2016Gli2 Antibody for Western Blotting Ganesh PusapatiThe goal of this experiment was to test the expression levels of Gli2 protein in NIH3T3 cells.

GLI-2 Antibody

GLI-2 Antibody

GLI-2 Antibody

GLI-2 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (57)

Reviews

GLI-2 Antibody from R&D Systems, a Bio-Techne Brand

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