Fig 2: Anti-apoptosis effect of Etpost (2 µg/ml) on the isolated sections of rat heart tissue. Anti-apoptosis effect of ethyl acetate fraction from P. reptans on the isolated sections of rat heart tissue. The effect of IR, L-NAME, Etpost (2 µg/ml) and Etpost (2 µg/ml) + L-NAME on apoptotic markers. A Expression of BAX and BCl-2 proteins after nuclei staining with DAPI and obtained from fluorescence microscope (observed under 400 magnifications). B Expression of the levels of caspase-3 protein. C Representative TUNEL stained and captured by fluorescence microscopy of rat heart sections with IR and Etpost (2 µg/ml) groups. The green nuclear structures represent apoptotic cells. The nuclei are stained by PI. The data were expressed as mean ± SEM. *P < 0.05 vs. IR group. Etpost: ethyl acetate fraction of P. reptans root

Fig 3: Western blot analysis. A Western blot analysis of BCl-2, BAX and levels of cleaved caspase-3 expression in heart tissue of Etpost (2 µg/ml) adjusted relative to GAPDH and IR. B Western blot analysis of GSK-3ß and SGK1 expression in the heart tissue of Etpost (2 µg/ml) adjusted relative to GAPDH and IR. GAPDH was used as a loading control. The data were expressed as mean ± SEM. *P < 0.05 vs. IR group. Etpost: ethyl acetate fraction of P. reptans root

Fig 4: Expression PVT1, miR-424-5p, CARM1, MMP-2, MMP-9, Bcl-2, and Bax in NSCLC (n = 80) and Adjacent Tissues(A) miR-424-5p expression and mRNA expression of PVT1, CARM1, MMP-2, MMP-9, Bcl-2, and Bax in tissues. (B) Western blot analysis for protein expression. (C) Protein expression of PVT1, CARM1, MMP-2, MMP-9, Bax, and Bcl-2 in tissues. *p < 0.05, compared with the adjacent tissues. Data are means ± SD from three independent experiments and analyzed by paired t test. PVT1, plasmacytoma variant translocation 1; miR-424-5p, mircoRNA-424-5p; CARM1, co-activator-associated arginine methyltransferase 1; MMP-2, matrix metallopeptidase-2; MMP-9, matrix metallopeptidase; Bcl-2, B cell lymphoma 2; Bax, Bcl2-associated X protein; NSCLC, non-small cell lung cancer.

Fig 5: Overexpression of miR-92b alleviates LPS-induced apoptosis. BEND cells were transfected with miR-92b mimic for 24 h, and then treated with 10 µg/mL LPS for another 24 h. (A) The protein levels of Bax and Bcl-2 were determined by Western blot. (B) The cell apoptosis was evaluated by flow cytometry. (C) The cell cycle was determined by flow cytometry. Data are expressed as mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01.