MANF Antibody AF3748 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMANF Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF3748
NCBI Gene AliasesARP, ARMET
ApplicationsImmunoprecipitation,Western Blot,Simple Western,Immunocytochemistry, ELISA Capture
Conjugate/TagUnconjugated
IsotypeIgG
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman,Mouse,Rat
HostGoat
Quantity100 ug (also 25 ug)
TargetMANF
NCBI Full Gene Namemesencephalic astrocyte derived neurotrophic factor
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human MANF, Leu22-Leu179, Accession # P55145
ConcentrationLYOPH
PurityAntigen Affinity-purified
ValidationKnockout Validated
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human MANF in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) MANF protein expression is upregulated in immune cells in the ischemic human brain and systemic recombinant MANF delivery in rat ischemic stroke model demonstrates anti-inflammatory effectsActa neuropathologica communicationsJanuary 16, 2024Anttila JE, Mattila OS, Liew HK, Mätlik K, Mervaala E, Lindholm P, Lindahl M, Lindsberg PJ, Tseng KY, Airavaara M.knockout tissues. In brief, 96-well MaxiSorp plates were coated with goat anti-human MANF antibody (AF3748, R&D Systems) in 50 mM carbonate buffer, pH 9.6. After blocking with 1% casein-PBST (PBS; 0.05% Tween

(2) MANF regulates neuronal survival and UPR through its ER-located receptor IRE1αCell reportsFebruary 28, 2023Kovaleva V, Yu LY, Ivanova L, Shpironok O, Nam J, Eesmaa A, Kumpula EP, Sakson S, Toots U, Ustav M, et al.

(3) CDNF Interacts with ER Chaperones and Requires UPR Sensors to Promote Neuronal SurvivalInternational journal of molecular sciencesAugust 22, 2022Eesmaa A, Yu LY, Göös H, Danilova T, Nõges K, Pakarinen E, Varjosalo M, Lindahl M, Lindholm P, Saarma M.recognized MANF in mouse tissue samples [80]. mMANF ELISA was built using goat antibodies to MANF (AF3748, R&D Systems) for coating, rabbit anti-MANF antibodies (LS-B2688, LSBio, Seattle, WA, USA) for detection,

(4) Human-Specific Regulation of Neurotrophic Factors MANF and CDNF by microRNAsInternational journal of molecular sciencesSeptember 7, 2021Konovalova J, Gerasymchuk D, Arroyo SN, Kluske S, Mastroianni F, Pereyra AV, Domanskyi A.Fisher Scientific, Waltham, MA, USA) was coated overnight at +4 °C with goat anti-hMANF pAb (1 µg/mL; AF3748, R&D Systems, Minneapolis, MN, USA) in carbonate coating buffer (35 mM NaHCO3, 15 mM Na2CO3, pH 9.6).

(5) Activation of the ATF6 (Activating Transcription Factor 6) Signaling Pathway in Neurons Improves Outcome After Cardiac Arrest in MiceJournal of the American Heart AssociationJune 15, 2021Shen Y, Li R, Yu S, Zhao Q, Wang Z, Sheng H, Yang W.Biotechnology OS9 (12497) Rabbit Cell Signaling Technology Derl1 (NB100-448) Rabbit Novus Biologicals MANF (AF3748) Goat Novus Biologicals FLAG (F3165) Mouse Sigma β-actin (A3854) Mouse Sigma Table S4. Expression

(6) Mesencephalic Astrocyte-Derived Neurotrophic Factor Is Upregulated with Therapeutic Fasting in Humans and Diet Fat Withdrawal in Obese MiceScientific reportsOctober 4, 2019Galli E, Rossi J, Neumann T, Andressoo JO, Drinda S, Lindholm P.earlier16. Briefly, the human MANF sandwich ELISA is built on goat anti-human MANF coating antibody (AF3748, R&D Systems), and horseradish peroxidase (HRP)-conjugated mouse anti-human MANF detection antibody

(7) Increased Serum Levels of Mesencephalic Astrocyte-Derived Neurotrophic Factor in Subjects With Parkinson's DiseaseFrontiers in neuroscienceJanuary 1, 2019Galli E, Planken A, Kadastik-Eerme L, Saarma M, Taba P, Lindholm P.Diluted samples and standards were applied to 96-well plates coated with goat anti-human MANF antibody (AF3748, R&D Systems), and incubated overnight at +4°C in agitation. After washing with PBST, bound MANF on

(8) Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) Is Highly Expressed in Mouse Tissues With Metabolic FunctionFrontiers in endocrinologyJanuary 1, 2019Danilova T, Galli E, Pakarinen E, Palm E, Lindholm P, Saarma M, Lindahl M.specific for MANF. Briefly, a 96-well plate (MaxiSorp, Nunc) was coated with goat antibodies to MANF (AF3748, R&D+ systems) in carbonate coating buffer (pH 9.6) for overnight at 4°C. Next day, after washing with

(9) Increased circulating concentrations of mesencephalic astrocyte-derived neurotrophic factor in children with type 1 diabetesScientific reportsJune 30, 2016Galli E, Härkönen T, Sainio MT, Ustav M, Toots U, Urtti A, Yliperttula M, Lindahl M, Knip M, Saarma M, et al.Scientific) 96-well plates were coated overnight at +4 °C with goat anti-human MANF polyclonal antibody (AF3748, R&D Systems) at 1 μg/ml in 50 mmol/l carbonate coating buffer (35 mmol/l sodium bicarbonate, 15 mmol/l

(10) Mesencephalic astrocyte-derived neurotrophic factor protects the heart from ischemic damage and is selectively secreted upon sarco/endoplasmic reticulum calcium depletionThe Journal of biological chemistryJuly 27, 2012Glembotski CC, Thuerauf DJ, Huang C, Vekich JA, Gottlieb RA, Doroudgar S.

(11) Mesencephalic astrocyte-derived neurotrophic factor is an ischemia-inducible secreted endoplasmic reticulum stress response protein in the heartCirculation researchNovember 21, 2008Tadimalla A, Belmont PJ, Thuerauf DJ, Glassy MS, Martindale JJ, Gude N, Sussman MA, Glembotski CC.confocal microscopy was carried out as previously described.10,17 The MANF antibody (R&D Systems, catalog no. AF3748) was used at 1:25. Immunoblots were performed as previously described10 using the MANF antibody at 1:2000. MANF

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MANF Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (11)

Reviews

MANF Antibody from R&D Systems, a Bio-Techne Brand

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