KLF5 Antibody AF3758 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemKLF5 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF3758
NCBI Gene AliasesCKLF, IKLF, BTEB2
ApplicationsImmunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
ReactivityHuman,Mouse
IsotypeIgG
TargetKLF5
HostGoat
NCBI Full Gene NameKLF transcription factor 5
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human KLF5, Leu85-Ala177, Accession # Q13887
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
ValidationKnockout Validated

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human and mouse KLF5 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) KLF4, rhKLF6, recombinant mouse (rm) KLF4 and rmKLF15 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Analysis of the relationship between central adiposity and biomechanical, histological, and immunohistochemical properties of the anterior wall of abdominal aortic aneurysmsJVS-Vascular ScienceFebruary 27, 2025Alexandre Malta Brandão, Marcos Vinícius Melo de Oliveira, Gina Camillo Rocha Silvestre, Alexandre Queiroz Silva, Michele Alberto Marques, Suely Aparecida Pinheiro Palomino, Maria de Lourdes Higuchi, Erasmo Simão da Silvamonocytes.•Peroxisome proliferator activated receptor-γ PPAR-γ) (BS-4590R, Bioss Antibody, Woburn, MA) and KLF5 (AF3758, R&D Systems, Minneapolis, MN): to evaluate adipogenic differentiation.11,24, 25, 26•TNF-alpha (NB600-587,

(2) Comparative analysis between abdominal aortic aneurysm and popliteal artery aneurysmJVS-Vascular ScienceJanuary 1, 2025Marcos Vinícius Melo de Oliveira, Alexandre Malta Brandão, Gina Camillo Rocha Silvestre, Alexandre Queiroz Silva, Michele Alberto Marques, Marcia Martins Reis, Maria de Lourdes Higuchi, Erasmo Simão da SilvaCarlsbad, CA; dilution 1:200) and KLF5 (anti-Human/Mouse KLF5 antibody, polyclonal goat IgG, catalog number AF3758, R&D Systems, Minneapolis, MN; dilution 1:200) were used to evaluate tissue adipogenic differentiation.15,26,

(3) Stem cell activity-coupled suppression of endogenous retrovirus governs adult tissue regenerationCellDecember 26, 2024Ying Lyu, Soo Jin Kim, Ericka S. Humphrey, Richa Nayak, Yinglu Guan, Qingnan Liang, Kun Hee Kim, Yukun Tan, Jinzhuang Dou, Huandong Sun, et al.D4M8R "Anti-SETDB1, rabbit polyclonal for WB",GeneTex GTX115305,GTX115305 "Anti-KLF5, goat polyclonal",R&D Systems AF3758,RRID: AB_2130246 "Anti-KRT6, rabbit polyclonal",BioLegend Poly19057,RRID: AB_2565052 "Anti-KRT14,

(4) BRD4-specific PROTAC inhibits basal-like breast cancer partially through downregulating KLF5 expressionOncogeneSeptember 1, 2024Kong Y, Lan T, Wang L, Gong C, Lv W, Zhang H, Zhou C, Sun X, Liu W, Huang H, et al.Cell Signaling Technology. Antibody for BRD3 (#ab264294; 1:2000) were purchased from abcam. Antibodies for KLF5 (#AF3758, 1:1000) and FGF-BP1 (MAB1593; 1:500) were purchased from R&D Systems. Cell culture MCF10A, 184B5,

(5) A reversible epigenetic memory of inflammatory injury controls lineage plasticity and tumor initiation in the mouse pancreasDevelopmental CellDecember 5, 2023David J. Falvo, Adrien Grimont, Paul Zumbo, William B. Fall, Julie L. Yang, Alexa Osterhoudt, Grace Pan, Andre F. Rendeiro, Yinuo Meng, John E. Wilkinson, et al.Polyclonal anti-KLF4,R&D Systems,"Cat# AF3158, RRID:AB_2130245" Goat Polyclonal anti-KLF5,R&D Systems,"Cat# AF3758, RRID:AB_2130246" Rabbit Polyclonal anti-Nestin,Sigma-Aldrich,"Cat# N5413, RRID:AB_1841032" Rabbit

(6) Krüppel-like Factor 5 Plays an Important Role in the Pathogenesis of Chronic PancreatitisCancersNovember 15, 2023Alavi M, Mejia-Bautista A, Tang M, Bandovic J, Rosenberg AZ, Bialkowska AB.overnight at 4 °C with a gentle shake. Primary antibodies: anti-KLF5 (R&D Systems, Minneapolis, MN, USA, AF3758), anti-KRT19 (Developmental Studies Hybridoma Bank, Iowa City, IA, USA, TROMAIII), anti-Amylase (Santa Cruz Biotechnology

(7) KLF5 inhibition potentiates anti-PD1 efficacy by enhancing CD8(+) T-cell-dependent antitumor immunityTheranosticsJanuary 1, 2023Wu Q, Liu Z, Gao Z, Luo Y, Li F, Yang C, Wang T, Meng X, Chen H, Li J, et al.(w:v in TBS), followed by overnight incubation at 4 °C with primary antibodies specific for KLF5 (#AF3758; 1:1000; R&D Systems), COX2 (#66351-1-Ig; 1:1000; Proteintech), CyclinD1 (#55506; 1:1000; Cell Signaling Technology

(8) Targeting the KLF5-EphA2 axis can restrain cancer stemness and overcome chemoresistance in basal-like breast cancerInternational journal of biological sciencesJanuary 1, 2023Zhao P, Sun J, Huang X, Zhang X, Liu X, Liu R, Du G, Gan W, Yang C, Tang Y, et al.1 h at room temperature and then incubated with the indicated primary antibodies (KLF5, R&D system AF3758; EphA2, Santa Cruz sc-398832; pS897 EphA2, Affinity AF7279; GAPDH, Santa Cruz sc-32233; β-actin, Sigma

(9) Histone Deacetylase Inhibitors (HDACi) Promote KLF5 Ubiquitination and Degradation in Basal-like Breast CancerInternational journal of biological sciencesJanuary 1, 2022Kong Y, Ren W, Fang H, Shah NA, Shi Y, You D, Zhou C, Jiang D, Yang C, Liang H, et al.KLF5 was detected by western blotting using an anti-acetylated lysine antibody (Minneapolis, MN. Cat# AF3758); Ac-KLF5K369 (1:1000 in 5% BSA) antibodies were generated in previous studies 36, 48. The anti-human

(10) Novel Antibody Exerts Antitumor Effect through Downregulation of CD147 and Activation of Multiple Stress SignalsJournal of oncologyJanuary 1, 2022Fukuchi K, Nanai K, Yuita H, Maru C, Tsukada J, Ishigami M, Nagai Y, Nakano Y, Yoshimura C, Yoneda K, et al.electrophoresis device (Wes; ProteinSimple) was used for western blotting analysis. Anti-KLF5 antibodies (AF3758; R&D Systems, Minneapolis, MN, US) were used to detect KLF5 protein. The anti-β-actin antibody (#4967L;

(11) Lgr6 marks epidermal stem cells with a nerve-dependent role in wound re-epithelializationCell stem cellSeptember 2, 2021Huang S, Kuri P, Aubert Y, Brewster M, Li N, Farrelly O, Rice G, Bae H, Prouty S, Dentchev T, et al.Labs), anti-TH (1:100, MAB7566, R&D Systems), anti-KLF4 (1:50, AF3158, R&D Systems), anti-KLF5 (1:50, AF3758, R&D Systems), anti-LRIG1 (1:50, AF3688, R&D Systems) and anti-BrdU (1:100, B-35128, Invitrogen). The

(12) Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cellsNature biotechnologyApril 1, 2020Hogrebe NJ, Augsornworawat P, Maxwell KG, Velazco-Cruz L, Millman JR.anti-PRSS1/2/3 (R&D Systems, AF3586), 1:100 mouse-anti-KRT19 (Dako, MO888), goat anti-KLF5 (R&D Systems, AF3758), rabbit anti-CDX2 (Abcam, ab76541), mouse anti-AFP (Abcam, ab3980), and rabbit anti-albumin (Abcam,

(13) KLF5 Is Crucial for Androgen-AR Signaling to Transactivate Genes and Promote Cell Proliferation in Prostate Cancer CellsCancersMarch 21, 2020Li J, Zhang B, Liu M, Fu X, Ci X, A J, Fu C, Dong G, Wu R, Zhang Z, et al.sonication, chromatin extracts were immunoprecipitated using anti-AR (06-680-AF488, Millipore), anti-KLF5 (AF3758, R&D Systems) or anti-IgG antibody. ChIP products were detected by regular PCR (with input loading quantity

(14) The aging skin microenvironment dictates stem cell behaviorProceedings of the National Academy of Sciences of the United States of AmericaMarch 10, 2020Ge Y, Miao Y, Gur-Cohen S, Gomez N, Yang H, Nikolova M, Polak L, Hu Y, Verma A, Elemento O, et al.

(15) HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cellsNature immunologyMarch 1, 2020Wang Y, Lifshitz L, Gellatly K, Vinton CL, Busman-Sahay K, McCauley S, Vangala P, Kim K, Derr A, Jaiswal S, et al.

(16) Krüppel-like Factor 5 Regulates Stemness, Lineage Specification, and Regeneration of Intestinal Epithelial Stem CellsCellular and molecular gastroenterology and hepatologyJanuary 1, 2020Kim CK, Saxena M, Maharjan K, Song JJ, Shroyer KR, Bialkowska AB, Shivdasani RA, Yang VW.Antibody,Source,Catalog # Goat polyclonal anti-KLF5 (used at 1:300),R&D Systems,Cat# AF3758 Rabbit polyclonal anti-KLF5 (used at 1:300),Abcam,Cat# Ab137676 Chicken polyclonal anti-GFP (used

(17) HDAC-mediated deacetylation of KLF5 associates with its proteasomal degradationBiochemical and biophysical research communicationsJune 7, 2018Tao R, Zhang B, Li Y, King JL, Tian R, Xia S, Schiavon CR, Dong JT.

(18) Stem Cell Lineage Infidelity Drives Wound Repair and CancerCellMay 4, 2017Ge Y, Gomez NC, Adam RC, Nikolova M, Yang H, Verma A, Lu CP, Polak L, Yuan S, Elemento O, et al.

(19) KLF13 regulates the differentiation-dependent human papillomavirus life cycle in keratinocytes through STAT5 and IL-8OncogeneOctober 20, 2016Zhang W, Hong S, Maniar KP, Cheng S, Jie C, Rademaker AW, Krensky AM, Clayberger C.

(20) The intestinal epithelial cell differentiation marker intestinal alkaline phosphatase (ALPi) is selectively induced by histone deacetylase inhibitors (HDACi) in colon cancer cells in a Kruppel-like factor 5 (KLF5)-dependent mannerThe Journal of biological chemistrySeptember 5, 2014Shin J, Carr A, Corner GA, Tögel L, Dávalos-Salas M, Tran H, Chueh AC, Al-Obaidi S, Chionh F, Ahmed N, et al.

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KLF5 Antibody

KLF5 Antibody

KLF5 Antibody

KLF5 Antibody

KLF5 Antibody

KLF5 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (22)

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KLF5 Antibody from R&D Systems, a Bio-Techne Brand

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