PD-1 Antibody

Product Specs
ItemPD-1 Antibody - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $479.00Supplier Page View Company Product Page
Catalog NumberNBP1-77276
ReactivityHuman,Mouse,Rat
TargetPD-1
HostRabbit
ImmunogenAntibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human PD-1. The immunogen is located within amino acids 210 - 260 of PD-1
Conjugate/TagUnconjugated
FormatPBS
NCBI Gene AliaseshPD-l, CD279, PD-1, PD1, hSLE1, SLEB2, hPD-1
Quantity0.1 mg (also 0.025 mg)
ApplicationsFlow Cytometry,ELISA,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunocytochemistry/ Immunofluorescence
Concentration1 mg/ml
NCBI Full Gene Nameprogrammed cell death 1
ClonalityPolyclonal
PurityImmunogen affinity purified
Molecular Weight32 kDa
Research AreaApoptosis, Stem Cell Markers
IsotypeIgG
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles
Antibody TypePrimary Antibodies

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Sizes Available: 0.1 mg (also 0.025 mg)

Questions and Answers

Can this PD-1 antibody be used for immunohistochemical studies in liver tissues?

Yes, the PD-1 antibody has been utilized for immunohistochemical staining in liver biopsies, as demonstrated in a study evaluating its expression in children with autoimmune hepatitis. The antibody was used at a dilution of 1:200 for this application, indicating its suitability for such studies ().

Is there any evidence supporting the use of this antibody in cancer research?

The PD-1 antibody has been referenced in research related to cancer immunotherapy, particularly in studies exploring its role in enhancing CD8+ T cell-mediated antitumor immunity. It has been shown to work synergistically with other therapies targeting PD-1, suggesting its relevance in cancer research ().

What species does this antibody react with, and how might that influence my experimental design?

This PD-1 antibody is reactive with mouse, human, and rat tissues. This broad reactivity allows for its application in various experimental designs, including comparative studies across species, which can be particularly useful in translational research settings.

What is the recommended storage condition for this antibody to maintain its stability?

The PD-1 antibody should be stored at 4°C for short-term use and aliquoted for long-term storage at -20°C. It is important to avoid freeze-thaw cycles to maintain the integrity of the antibody.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) Peptide-mediated inhibition of aberrant chaperone-mediated autophagy in pericytes prevents glioblastoma progression through MAPT/tau secretionAutophagyMay 18, 2026Maria Dolores Salinas, Isabel Maria Martínez, Elena Naranjo, Maria Luisa Molina, Ana Maria Cuervo, Sylviane Muller, Rut Valdor45–0042-82), FOXP3 (eBioscience, 17–5773-82), CTLA4 (eBioscience, 17–1522-82) and PDCD1/PD-1 (Novus, NBP1-77276). Background nonspecific fluorescence was measured using control isotype antibodies. Labeled cells were

(2) Integrating gene demethylation and immune modulation: PD-1 nanovesicles as a dual-action therapy for NSCLCMater Today BioMay 9, 2025Heng Zhang, Yuan Liang, Shiyang Deng, Jiang Duincubating with primary antibodies overnight at 4 °C. The primary antibodies used were PD-1 (1:1500; NBP1-77276; Novus Biologicals), PD-L1 (1:1000; NBP1-76769; Novus Biologicals), Na/K ATPase (1:1000; #3010; Cell Signaling Technology

(3) CCR4 Blockade Depletes Regulatory T Cells and Prolongs Survival in a Canine Model of Bladder CancerCancer immunology researchJanuary 1, 0001"Maeda S, Murakami K, Inoue A, Yonezawa T, Matsuki N."

(4) GPNMB(+) Gal-3(+) hepatic parenchymal cells promote immunosuppression and hepatocellular carcinogenesisThe EMBO journalJanuary 1, 0001"Meng Y, Zhao Q, Sang Y, Liao J, Ye F, Qu S, Nie P, An L, Zhang W, Jiao S, et al."(MA),bs‐1351R,Flow Cyto Anti‐IFN gamma antibody,Bioss (MA),bs‐0480R,Flow Cyto Anti‐PD1 antibody,Novus (CO),NBP1‐77276,Flow Cyto Anti‐EpCAM antibody,Biorbyt (MO),orb15573,Flow Cyto Anti‐IFN gamma antibody,Biolegend (CA),502526,Flow

(5) Inhibiting ACK1-mediated phosphorylation of C-terminal Src kinase counteracts prostate cancer immune checkpoint blockade resistanceNature communicationsJanuary 1, 0001"Sridaran D, Chouhan S, Mahajan K, Renganathan A, Weimholt C, Bhagwat S, Reimers M, Kim EH, Thakur MK, Saeed MA, et al."

(6) In vitro characterization of a small molecule PD-1 inhibitor that targets the PD-l/PD-L1 interactionScientific reportsJanuary 1, 0001"Lu CH, Chung WM, Tsai CH, Cheng JC, Hsu KC, Tzeng HE."maintained in a humidified 5% CO2 environment at 37 °C. PD-1 antibody was used in this study (Cat No. NBP1-77276, Novus Biologicals, USA). Dataset preparation and virtual screening Human PD-1 (Protein Data Bank

(7) A Nanovaccine Based on Adjuvant Peptide FK-13 and l-Phenylalanine Poly(ester amide) Enhances CD8(+) T Cell-Mediated Antitumor Immunity"Advanced science (Weinheim, Baden-Wurttemberg, Germany)"January 1, 0001"Xie C, You X, Zhang H, Li J, Wang L, Liu Y, Wang Z, Yao R, Tong T, Li M, et al."EEA1 (ab2900) were from Abcam. Anti‐PD‐1 for immunohistochemical staining was from Novus Biologicals (NBP1‐77276), and anti‐PD‐1 antibody for the animal experiment was from BioXcell (BE0273). The protein ladder (DB180)

(8) "Hepatic expression of programmed death-1 (PD-1) and its ligand, PD-L1, in children with autoimmune hepatitis: relation to treatment response"Clinical and experimental hepatologyJanuary 1, 0001"Agina HA, Ehsan NA, Abd-Elaziz TA, Abd-Elfatah GA, Said EM, Sira MM."placed on positive charged slides. The primary antibody used were rabbit polyclonal PD-1 antibody (NBP1-77276; Novus Biologicals, Littleton, CO 80120, USA) at a dilution of 1 : 200 or rabbit polyclonal PD-L1 antibody

(9) "Review of article: Rivaroxaban with or without aspirin in stable cardiovascular disease. Eikelboom JW, Connolly SJ, Bosch J, et al. for the COMPASS investigators"Journal of vascular nursing : official publication of the Society for Peripheral Vascular NursingJanuary 1, 0001Kohlman-Trigoboff D.

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PD-1 Antibody - BSA Free

PD-1 Antibody - BSA Free

PD-1 Antibody - BSA Free

PD-1 Antibody - BSA Free

PD-1 Antibody - BSA Free from Novus Biologicals, a Bio-Techne Brand

Specifications/Features

Questions and Answers

Novus Biologicals, a Bio-Techne Brand

Citations (9)

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PD-1 Antibody - BSA Free from Novus Biologicals, a Bio-Techne Brand

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