Immunocytochemistry/Immunofluorescence analysis with Stressmarq’s Rabbit Anti-Rat SAP97 Monoclonal Antibody, Clone G28 (SMC-631). Tissue: Pheochromocytoma cells (PC-12). Species: Rat. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Rat SAP97 Recombinant Monoclonal [G28] (SMC-631) at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-Rabbit IgG-AlexaFluor488 (green) at 1:1000 for 1 hour at RT in the dark. Counterstain: DAPI (blue) nuclear stain at 1:1000 for 5 min at RT in the dark.

Immunocytochemistry/Immunofluorescence analysis with Stressmarq’s Rabbit Anti-Rat SAP97 Monoclonal Antibody, Clone G28 (SMC-631). Tissue: Neuroblastoma cells (Kelly). Species: Human. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Rat SAP97 Recombinant Monoclonal [G28] (SMC-631) at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-Rabbit IgG-AlexaFluor488 (green) at 1:1000 for 1 hour at RT in the dark. Counterstain: DAPI (blue) nuclear stain at 1:1000 for 5 min at RT in the dark.

Western blot analysis with Stressmarq’s Rabbit Anti-Rat SAP97 Monoclonal Antibody, Clone G28 (SMC-631) showing detection of Human SAP97 protein in HeLa whole cell lysate. Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Rat SAP97 Recombinant Monoclonal [G28] (SMC-631) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min at RT in the dark. Exposed 5 seconds.

Western blot analysis with Stressmarq’s Rabbit Anti-Rat SAP97 Monoclonal Antibody, Clone G28 (SMC-631) showing detection of Mouse SAP97 protein in Mouse Brain lysate. Block: 5% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Rat SAP97 Recombinant Monoclonal [G28] (SMC-631) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 3 min at RT in the dark. Exposed 1 second.