Fig 1: NPCs accumulate on ALL nuclear lines in an approximately 1µm wide region flanking the ALL lines, and are brighter fluorescently along ALL lines. Cells were subjected to cyclic stretch, then fixed and antibody labeled. 10 pixel wide linescans for fluorescent intensity are measured along the yellow bar. Scale bars are all 5µm. Grayed regions on the graphs show the 1µm wide region flanking the ALL line. Yellow arrows show overlaid SF that do not form ALL lines. A. Actin and LaminA/C align on the dorsal surface of the nucleus. Nucleoporin TPR shows accumulation of NPC flanking the actin and LaminA/C line. The image and accompanying graph of linescan clearly shows that not all actin SF closely apposed to the nuclear surface form ALL lines. A’. Fluorescent intensity graph of linescan taken at the dashed yellow line using X position = 0 at the center of the ALL line. B. Actin and LaminA/C concentrate to form a linear structure. TPR labeled NPC flanking the actin/LaminA/C line can align with a railroad track orientation along the ALL line. B’. Fluorescent intensity graph of linescan taken at the dashed yellow line using X position = 0 at the center of the ALL line. C. Nucleoporin Pom121, also accumulates and is brighter in NPCs along ALL lines. C’. Fluorescent intensity graph of linescan taken at the dashed yellow line using X position = 0 at the center of the ALL line. D. LINC complex protein SUN2 and antibody FG414, which labels Nup153 and other FG-domain containing nucleoporins, also concentrates along ALL lines. D’. Fluorescent intensity graph of linescan taken at the dashed yellow line using X position = 0 at the center of the ALL line.