Spi-B Antibody AF7204 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemSpi-B Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $489.00Supplier Page View Company Product Page
Catalog NumberAF7204
Quantity100 ug (also 25 ug)
ApplicationsImmunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Intracellular Staining by Flow Cytometry,Immunocytochemistry,CyTOF-ready,Immunocytochemistry/ Immunofluorescence
IsotypeIgG
Conjugate/TagUnconjugated
HostSheep
NCBI Gene AliasesSpi-B, SPI-B
ReactivityMouse,Transgenic Mouse
TargetSpi-B
NCBI Full Gene NameSpi-B transcription factor, Spi-B transcription factor (Spi-1/PU.1 related)
ClonalityPolyclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenE. coli-derived recombinant mouse Spi-B, Tyr18-Glu167 (Tyr110Phe), Accession # O35906
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse Spi-B in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human Spi-B is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) SpiB regulates the expression of B-cell-related genes and increases the longevity of memory B cellsFrontiers in ImmunologyOctober 27, 2023Shu Horiuchi, Takuya Koike, Hirofumi Takebuchi, Katsuaki Hoshino, Izumi Sasaki, Yuri Fukuda-Ohta, Tsuneyasu Kaisho, Daisuke Kitamurato the manufacturer’s protocol (eBioscience) with anti-SpiB sheep polyclonal antibody (R&D systems, AF7204) followed by Alexa 647-labeled donkey anti-sheep IgG (Invitrogen, A-21448). All samples were analyzed

(2) ONECUT2 regulates RANKL-dependent enterocyte and microfold cell differentiation in the small intestine; a multi-omics studyNucleic acids researchFebruary 22, 2023Luna Velez MV, Neikes HK, Snabel RR, Quint Y, Qian C, Martens A, Veenstra GJC, Freeman MR, van Heeringen SJ, Vermeulen M.mounting media (Fisher Chemical). Overnight incubation with sheep anti-SPIB antibody (R&D Systems, AF7204, RRID:AB_10995033) diluted 1:100 in blocking solution was followed by a 1-hour incubation with Alexa Fluor 568 Donkey anti-Sheep IgG (H + L

(3) The gut microbiota induces Peyer's-patch-dependent secretion of maternal IgA into milkCell reportsSeptember 7, 2021Usami K, Niimi K, Matsuo A, Suyama Y, Sakai Y, Sato S, Fujihashi K, Kiyono H, Uchino S, Furukawa M, et al.

(4) Osteoprotegerin-dependent M cell self-regulation balances gut infection and immunityNature communicationsJanuary 13, 2020Kimura S, Nakamura Y, Kobayashi N, Shiroguchi K, Kawakami E, Mutoh M, Takahashi-Iwanaga H, Yamada T, Hisamoto M, Nakamura M, et al.anti-GP2 antibody (D278-3; MBL, 1:400); anti-Tnfaip2 antibody (Kimura et al.14: 200); anti-SpiB antibody (AF7204; R&D Systems, 1:200); anti-RelB antibody (sc-226; Santa Cruz Biotechnology, 1:100); and anti-RANKL antibody

(5) Gut-Innervating Nociceptor Neurons Regulate Peyer's Patch Microfold Cells and SFB Levels to Mediate Salmonella Host DefenseCellJanuary 9, 2020Lai NY, Musser MA, Pinho-Ribeiro FA, Baral P, Jacobson A, Ma P, Potts DE, Chen Z, Paik D, Soualhi S, et al.overnig ht at 4°C in primary antibodies (rat anti-GP2, MBL D278–3, 1:500; sheep anti-SpiB, R&D Systems AF7204, 1:50) diluted in 0.1% Triton X-100 / PBS in 96-well plates with gentle shaking. Tissues were washed

(6) The role of CSF1R-dependent macrophages in control of the intestinal stem-cell nicheNature communicationsMarch 28, 2018Sehgal A, Donaldson DS, Pridans C, Sauter KA, Hume DA, Mabbott NA.

(7) Development of intestinal M cells and follicle-associated epithelium is regulated by TRAF6-mediated NF-κB signalingThe Journal of experimental medicineFebruary 5, 2018Kanaya T, Sakakibara S, Jinnohara T, Hachisuka M, Tachibana N, Hidano S, Kobayashi T, Kimura S, Iwanaga T, Nakagawa T, et al.

(8) c-Rel is dispensable for the differentiation and functional maturation of M cells in the follicle-associated epitheliumImmunobiologyFebruary 1, 2017Sehgal A, Kobayashi A, Donaldson DS, Mabbott NA.

(9) Increased Abundance of M Cells in the Gut Epithelium Dramatically Enhances Oral Prion Disease SusceptibilityPLoS pathogensDecember 1, 2016Donaldson DS, Sehgal A, Rios D, Williams IR, Mabbott NA.

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Spi-B Antibody

Spi-B Antibody

Spi-B Antibody

Spi-B Antibody

Spi-B Antibody

Spi-B Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (9)

Reviews

Spi-B Antibody from R&D Systems, a Bio-Techne Brand

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