Western blot was performed using Anti-TDP-43 Polyclonal Antibody(Product # PA5-17011) and a 44kDa band corresponding to TDP-43 was observed across cell lines and tissue extract tested. A non-specific band around 80 kDa has also been observed. Whole Cell Extract-WCL (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), Caco-2 (Lane 3), K-562 (Lane 4), Neuro-2a (Lane 5), NIH/3T3 (Lane 6) and tissue extract of Mouse Thymus (Lane 7), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).