Fig 1: Downregulation of ENC1 inhibits the proliferation of NSCLC cells. (A and B) Effects of ENC1 deficiency on the proliferation were investigated in A549 and H1299 by CCK-8 assay. (C-F) The effects of ENC1 deficiency on the proliferation were investigated in A549 and H1299 using an EdU detection kit. *P<0.05, **P<0.01 vs. si-NC group. ENC1, ectodermal-neural cortex 1; NSCLC, non-small cell lung cancer.
Fig 2: Downregulation of ENC1 affects the JNK and ERK pathways. (A-C) Effects of ENC1 deficiency on the expression of p-ERK, ERK, p-JNK, JNK p-p38 and p38 were quantified in A549 and H1299 cells by western blot analysis. *P<0.05 vs. si-NC group. ENC1, ectodermal-neural cortex 1.
Fig 3: Upregulation of ENC1 enhances the proliferation, migration and invasion of NSCLC cells. (A) The overexpression efficiency of ENC1 was quantified in A549 and H1299 by RT-qPCR. (B) The overexpression efficiency of ENC1 was quantified in A549 and H1299 by western blot analysis. (C) Effects of ENC1 overexpression on proliferation were investigated in A549 and H1299 cells by CCK-8 assay. (D) The effects of ENC1 overexpression on the proliferation were investigated in A549 and H1299 cells using an EdU detection kit. (E and F) The effects of ENC1 overexpression on cell migration and invasion were investigated by Transwell assay. *P<0.05, **P<0.01, ***P<0.001 vs. OE-NC group. ENC1, ectodermal-neural cortex 1; NSCLC, non-small cell lung cancer.
Fig 4: High expression of ENC1 in lung cancer tissues and cells. (A and B) The expression of ENC1 between lung cancer and para-cancerous tissue from the TCGA database. (C) Kaplan-Meier survival plot of overall survival of lung cancer in TCGA database, categorized according to ENC1 gene expression (high vs. low, based on mean expression). (D) The expression of ENC1 in normal and tumor tissues by RT-qPCR. (E) The expression of ENC1 in tumor tissues between stage II and III by RT-qPCR. (F) The expression of ENC1 in normal and tumor tissues by immunohistochemical analysis. (G and H) The expression of ENC1 between normal cell line and lung cancer cell lines by RT-qPCR and western blot analysis. Scale bar, 50 µm; *P<0.05, ***P<0.001 vs. normal group; **P<0.01 vs. 16HBE cell group; ENC1, ectodermal-neural cortex 1.
Fig 5: Downregulation of ENC1 inhibits the migration and invasion of NSCLC cells. (A) Knockdown efficiency of ENC1 was quantified in A549 and H1299 by RT-qPCR. (B) Knockdown efficiency of ENC1 was quantified in A549 and H1299 by western blot analysis. (C-J) The effects of ENC1 deficiency on cell migration and invasion were investigated by Transwell assay. (K-M) The effects of ENC1 deficiency on the expression of N-cadherin, E-cadherin, MMP2 and MMP9 were investigated by western blot analysis. *P<0.05, **P<0.01, ***P<0.001 vs. si-NC group. ENC1, ectodermal-neural cortex 1; NSCLC, non-small cell lung cancer.
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