Fig 1: Monensin perturbs mitochondrial function and induces mitochondrial damage in TEM 4-18 cells. Cells were treated for 3 h to 72 h with monensin (10 nM). (a) Mitochondrial membrane potential (JC-1 staining) (n = 8) and (b,c) expression of BNIP3 (Western blot) were evaluated (n = 6). (b) shows a representative Western blot. The blot was cut based on migration of molecular weight markers and stained separately for BNIP3 and ß-actin. The images shown are cropped from sections from the blots shown in Supplementary Fig. S6. Data represent mean values ± SEM.*p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.0001.