Western Blot of Histone H3 K27me3 Antibody. Western Blot analysis against untreated cell extracts. Lane 1: Molecular Weight Marker. Lane 2: HeLa cell lysates (p/n W09-000-364). Lane 3: 3T3 cell lysates (p/n W10-000-358). Lane 4: PC12 cell lysates (p/n W12-001-GL9). Lane 5: A431 cell lysate (p/n W09-000-361). Primary antibody: Histone H3 K27me3 antibody at 1.0 µg/mL for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: (p/n MB-073) BlockOut overnight at 4°C. Predicted/Observed size: 15 kDa for Histone H3 K27Me3. Other band(s): none.