Fig 2: PFE interferes with lipid metabolism in mice's liver. (a) The ultrastructural changes of the liver mitochondria by transmission electron microscope, scale bars = 500 μm. Arrows indicate mitochondrial cristae. (b) Western blot analysis of ABCC3, COX6A1, CPT1A, SLC27A5, NDUFB5, and cytochrome C expressions in the liver of mice. The values were expressed as mean ± SD, n = 3. ∗P < 0.05 and ∗∗P < 0.01 compared to the control group.

Fig 3: Calcipotriol inhibits ductular reaction and modulates synthesis and transport of BA. (A) Detection of mRNA expression of CK-19 in liver samples with RT-PCR. (B) IHC staining of representative mouse liver samples for CK-19 and image quantification of CK-19 expression. Scale bar: 100 μm. (C) Detection of mRNA expression of CYP7A1, CYP8B1, MRP3, MRP4 and OST-α in liver samples with RT-PCR. (D) Detection of mRNA expression of MRP2, MRP3, MRP4, and OST-α in kidney samples with RT-PCR. All RT-PCR results were normalized against β-actin and expressed as 2-ΔΔCT. All data are presented as mean ± SEM (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001).

Fig 4: Identification of single clone cells L-2F7 for the epithelial signature. Epithelial markers were detected in L-2F7 cells, gallbladder cancer cell lines NOZ and GBC-SD, and L-G33 cells as a fibroblast control with flow cytometry (A). CK7, CK19, MRP3, fibronectin, and N-cadherin were determined with Western blot (B). L-2F7 cells were transfected by GFP-FLAG, then the expression of GFP and FLAG was detected by Western blot (C) and fluorescence was applied for observing the transfection efficiency (D). The sensitivity of puromycin in L-2F7 cells was measured by calculating IC50 from the fitted curve (E).