Fig 2: Effect of Mel on the expression of VEGFA. (A) Western blotting detection of VEGFA, VEGFR2, FSHR protein expression level in ovary. (B) Quantification analysis of the VEGFA, VEGFR2, FSHR protein expression level. (C) Immunofluorescence (IF) detection of VEGFA expression level in follicles at all stages. (D) Quantification analysis of the VEGFA expression level in 50–250 µm follicles. (E) Quantification analysis of the VEGFA expression level in 250–450 µm and >450 µm follicles. Horizontal bars denote treatments differing p < 0.05 *, p < 0.01 **.