Fig 1: TGFβ1 and miR-133a target ESRP1 pathways in airway epithelial cells. (a) Beas-2b cells were treated with 5 ng/ml TGFβ1 for the indicated time. (b) Beas-2b and NHBE cells were mock transfected, or transfected with control or miR-133a mimics. Cells were harvested 3 days later for western blot analysis of indicated proteins. Experiments were conducted at least three times, and representative results are shown. The grouped blots in (a) were cropped from different parts of the same gel. The anti-p120ctn and anti-GAPDH blots in (b) were cropped from different parts of the same gel. However, the anti-ESRP1/2 blot was from the same sample but a different gel. Unprocessed original scans of the blots are shown in Supplementary Fig. S5.
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