HIF-2alpha (D9E3) Rabbit mAb 7096S from Cell Signaling Technology

Product Specs
ItemHIF-2alpha (D9E3) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number7096S
ClonalityMonoclonal
NCBI Full Gene Nameendothelial PAS domain protein 1
ApplicationsWestern Blot (WB)
ReactivityHuman (Hu)
Research AreaDevelopmental Biology
IsotypeIgG
CloneD9E3
Molecular Weight120
NCBI Gene AliasesECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73
HostRabbit
TargetHIF2-alpha
Quantity100 µl

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) HIF2A as a prognostic and clinical therapeutic target in ovarian clear cell carcinomaInternational Journal of CancerJune 1, 2026Mengxin Jiang, Ken Yamaguchi, Kohei Hamada, Yuko Hosoe, Yuka Mise, Sachiko Kitamura, Zhihong Liu, Hairong Wei, Zhenhai Gao, Mana Taki, et al.blocking, the membranes were incubated overnight at 4°C with primary antibodies against HIF2A (D9E3, #7096, 1:1000 dilution, Cell Signaling Technology [CST], RRID: AB_10898028) and ACTB (#4967, CST, RRID: AB_330288

(2) Targeting and dissociating HIF2α from the molecular chaperone Hsp70 triggers apoptosis in kidney cancerCommunications MedicineJanuary 12, 2026Jennifer A. Heritz, Sarah J. Backe, Angela Pacherille, Sara J. Cayaban, Michael F. Basin, Britannia Smith, Rebecca A. Sager, Michael Daneshvar, Dawn E. Post, Mark R. Woodford, et al.achieved by incubating 1500 µg lysate with 1.5 µl anti-HIF2α antibody (Cell Signaling Technology, Cat#7096; RRID: AB_10898028) overnight followed by 25 µl protein G agarose (ThermoFisher Scientific, Cat# 15-920-010)

(3) HIF2α inhibits glutaminase clustering in mitochondria to sustain growth of clear cell renal cell carcinomaJCI InsightDecember 8, 2025Wencao Zhao, Sara M. Demczyszyn, Nathan J. Coffey, Yanqing Jiang, Boyoung Kim, Schuyler Bowers, Caitlyn Bowman, Michael C. Noji, Cholsoon Jang, M. Celeste Simon, et al.TIMM23 (Abcam, ab116329), DRP1 (Proteintech, 12957-1-AP), HIF1α (Novus Bio, NB100-296), HIF2α (CST, 7096s), MYC-tag (CST, 2278s), mCherry (CST, 43590), 14-3-3 (CST, 8312), and GAPDH (CST, 5174). Secondary antibodies

(4) Vascular Endothelial Growth Factor B Modulates Cardiac Functions via Ferroptosis Pathways in Post-Myocardial InfarctionCellsOctober 21, 2025Sai Manasa Varanasi, Ankit Sabharwal, Shreyartha Mukherjee, Huzaifa Muhammad, Riya Kar, Carter Magnano, Anya Dorairaj, Enfeng Wang, Shamit Dutta, Pritam Das, et al.(Sigma-Aldrich, Burlington, MA, USA). Incubation with the primary antibody (HIF1α (CST #37165s), HIF2α (CST #7096S), ERK (CST #9102S), pERK (CST #9101S), AKT (CST #9272S), pAKT (CST #9271S), BAX (CST #2772S), BCL2 (CST

(5) Long-Term Hypoxia Upregulates Wnt and TGFβ1 Signaling in Eccrine Sweat Gland Cells In VitroInt J Mol SciJuly 11, 2025Yanlin Lyu, Hiroko Kato, Qianwen Luo, Naoya Otani, Tateki Kubo, Kiyotoshi Sekiguchi, Fumitaka Fujitaantibodies used were HIF-1α (Cell Signaling Technology, 36169), HIF-2α (Cell Signaling Technology, 7096), αSMA (Abcam, ab5694), β-Catenin Cell Signaling Technology, 37447), Phospho-SMAD2 (Ser465/467)/SMAD3

(6) Oxygen-Releasing Nanodroplets Relieve Intratumoral Hypoxia and Potentiate Photodynamic Therapy in 3D Head and Neck Cancer SpheroidsACS Biomaterials Science & EngineeringApril 14, 2025Marvin Xavierselvan, Ronak Tarun Shethia, Brooke Bednarke, Vicky Yang, Leah Moses, Satya Siva Kishan Yalamarty, Jason Cook, Srivalleesha MallidiHIF-1α (dilution: 1:1000, Cat: 3716, Cell Signaling Technology), and HIF-2α (dilution: 1:1000, Cat: 7096, Cell Signaling Technology). 2.17 Statistical Analysis Data analysis was performed by using GraphPad

(7) CRISPR-Cas9 genetic screens reveal regulation of TMPRSS2 by the Elongin BC-VHL complexSci RepApril 7, 2025Ildar Gabaev, Alexandra Rowland, Emilija Jovanovic, Christian M. Gawden-Bone, Thomas W. M. Crozier, Ana Teixeira-Silva, Edward J. D. Greenwood, Pehuén Pereyra Gerber, Niek Wit, James A. Nathan, et al.rabbit-anti-HIF1b/ARNT (Cell Signaling Cat. No. 5537 T), rabbit-anti-HIF2a (Cell Signaling Cat. No. 7096S), rabbit anti-VHL (Cell Signaling Cat. No. 68547S), rabbit anti-TCEB2/Elongin-B (Abcam, Cat. No. EPR10440(B)),

(8) HIF regulates multiple translated endogenous retroviruses: Implications for cancer immunotherapyCellApril 3, 2025Qinqin Jiang, David A. Braun, Karl R. Clauser, Vijyendra Ramesh, Nitin H. Shirole, Joseph E. Duke-Cohan, Nancy Nabilsi, Nicholas J. Kramer, Cleo Forman, Isabelle E. Lippincott, et al.Biosystems c600 Imager. The primary antibodies used were rabbit α-HIF2α (Cell Signaling Technology 7096) at 1:1000, α-NDRG1 (Cell Signaling Technology 5196) at 1:1000, rabbit α-VHL (Cell Signaling Technology

(9) Hypoxia inducible factor 3-alpha promotes a malignant phenotype in colorectal cancer cellsIUBMB LifeFebruary 21, 2025Alejandro Lopez-Mejia, Angela Patricia Moreno-Londoño, Gabriela Fonseca Camarillo, Jesús Kazuo Yamamoto-Furusho, Juan Antonio Villanueva-Herrero, Jorge Luis de Leon-Rendón, Maria Cristina Castañeda Patlán, Martha Robles-Floresobtained from Novus Biologicals (Centennial, CO, USA). Rabbit anti‐HIF‐1α (3716) and anti‐ HIF‐2α (7096) were purchased from Cell Signaling Technology (Danvers, MA, USA). Mouse anti‐GAPDH (sc‐47724) was from

(10) Conserved patterns of transcriptional dysregulation, heterogeneity, and cell states in clear cell kidney cancerCell ReportsJanuary 28, 2025Olivia Lombardi, Ran Li, Faiz Jabbar, Hannah Evans, Silvia Halim, Joanna D.C.C. Lima, Lisa Browning, Helen M. Byrne, Hani Choudhry, Peter J. Ratcliffe, et al.antibodies used were anti-HIF-1α (BD cat no. 610959), anti-HIF-2α (in-house 190b or Cell Signaling cat no. 7096S), anti-HIF-1β (Novus Biologicals cat no. NB100-110 or Cell Signaling cat. no. 5537S), anti-CA9 (Abcam

(11) Hypoxia Compromises the Differentiation of Human Osteosarcoma Cells to CAR-R, a Hydroxylated Derivative of Lithocholic Acid and Potent Agonist of the Vitamin D ReceptorInt J Mol SciJanuary 3, 2025Haley Evans, Alexander Greenhough, Laura Perry, Gonzalo Lasanta, Carmen M. Gonzalez, Antonio Mourino, Jason P. MansellPaisley, UK). The antibodies used were as follows: HIF-1a (1:1000, BD, 610,959), HIF-2a (1:1000, CST, 7096), b-actin (1:10,000, Sigma, A5316), and VDR (1:1000, CST, 12,550). Following primary and HRP-conjugated

(12) E-Cadherin Induces Serine Synthesis to Support Progression and Metastasis of Breast CancerCancer ResearchSeptember 4, 2024Geonhui Lee, Claudia Wong, Anna Cho, Junior J. West, Ashleigh J. Crawford, Gabriella C. Russo, Bishwa Ranjan Si, Jungwoo Kim, Lauren Hoffner, Cholsoon Jang, et al.(#13428), c-Myc (#5605S), PDH (#3205S), phospho-PDH (#31866), Smad2/3 (#8685S), HIF-1α (#14179S), HIF-2α (#7096S), ASCT2 (#8057) and E-cadherin (#3195S, #14472S) antibodies were obtained from Cell Signaling Technology

(13) Deubiquitinating enzyme mutagenesis screens identify a USP43-dependent HIF-1 transcriptional responseThe EMBO JournalJuly 15, 2024Tekle Pauzaite, Niek Wit, Rachel V Seear, James A Nathan,BD Biosciences,610959 Rabbit monoclonal anti-HIF-1α,Cell Signalling,36169 Rabbit monoclonal anti-HIF-2α,Cell Signalling,7096 Rabbit monoclonal anti-HIF-1β,Cell Signalling,5537 Rabbit polyclonal anti-USP43,Atlas AB,HPA027762 Rabbit

(14) Kinome-wide siRNA screen identifies a DCLK2-TBK1 oncogenic signaling axis in clear cell renal cell carcinomaMolecular cellFebruary 15, 2024Hu L, Zhang Y, Guo L, Zhong H, Xie L, Zhou J, Liao C, Yao H, Fang J, Liu H, et al.,Cell Signaling Technology,Cat# 5483; RRID:AB_10693472 Rabbit anti- HIF2α,Cell Signaling Technology,Cat# 7096; AB_10898028 Rabbit anti-p62,Cell Signaling Technology,Cat# 39749; RRID:AB_2799160 Rabbit anti-DCLK1,Cell Signaling Technology

(15) Deletion of prolyl hydroxylase domain-containing enzyme 3 (phd3) in zebrafish facilitates hypoxia toleranceThe Journal of biological chemistryDecember 1, 2023Liao Q, Deng H, Wang Z, Yu G, Zhu C, Jia S, Liu W, Bai Y, Sun X, Chen X, et al.(#NB100-134) and anti-HIF-2α antibody (#NB100-122) were purchased from Novus. Anti-HIF-2α antibody (#7096) and anti-HIF-2α antibody (#59973) were purchased from Cell Signaling Technology. Anti-α-tubulin antibody

(16) ATR activation by Cr-DNA damage is a major survival response establishing late S and G2 checkpoints after Cr(VI) exposureToxicology and Applied PharmacologyOctober 15, 2023Sophia Valiente, Casey Krawic, Anatoly Zhitkovichphospho-AKT (4060), AKT (2938), DNAPK (12311), histone H3 (9715), phospho-H3 (9701), HIF1α (14179), HIF2α (7096), L7A (2415) FOXM1 (5436), SOD1 (2770), fibrillarin (2639), RAD17 (8561), phospho-RAD17 (6981), CDK1

(17) Disruption of sirtuin 7 in zebrafish facilitates hypoxia toleranceThe Journal of biological chemistryAugust 1, 2023Liao Q, Zhu C, Sun X, Wang Z, Chen X, Deng H, Tang J, Jia S, Liu W, Xiao W, et al.#NB100-122, Novus), anti-HIF-2α (1:1000, #59973, Cell Signaling Technology), anti-HIF-2α (1:1000, #7096, Cell Signaling Technology), anti-β-actin (1:100,000, #AC026, ABclonal), anti-H3 (1:2000, #A2348, ABclonal),

(18) New findings on the action of hypericin in hypoxic cancer cells with a focus on the modulation of side population cellsBiomedicine & PharmacotherapyJuly 1, 2023Viktória Buľková, Jana Vargová, Marián Babinčák, Rastislav Jendželovský, Zbyněk Zdráhal, Pavel Roudnický, Ján Košuth, Peter FedoročkoSanta Cruz Biotechnology, Dallas, TX, USA), HIF-1α (1:500; 610959; BD Biosciences), HIF-2α (1:1000; 7096; Cell Signaling Technology, Danvers, MA, USA), and β-actin (1:5000; A5441; Sigma-Aldrich). Subsequently,

(19) Association of NRF2 with HIF-2α-induced cancer stem cell phenotypes in chronic hypoxic conditionRedox biologyApril 1, 2023Hallis SP, Kim SK, Lee JH, Kwak MK.antibody (#610958) was purchased from BD Biosciences (Palo Alto, CA, USA). Antibodies against HIF-2α (#7096), KLF4 (#4038), and pVHL (#68547) were obtained from Cell Signaling Technology Inc. (Danvers, MA, USA).

(20) Ras-dependent activation of BMAL2 regulates hypoxic metabolism in pancreatic cancerbioRxivMarch 24, 2023H. Carlo Maurer, Alvaro Curiel-Garcia, Sam Holmstrom, Pasquale Laise, Carmine F. Palermo, Steven A. Sastra, Anthony Andren, Zhang Li, Tessa LeLarge, Irina Sagalovskiy, et al.Cell Signaling, #36169S), beta-Actin (1:1000, Cell Signaling, #4970S), HIF-2a (1:1000, Cell Signaling, #7096S), BMAL2 (1:500, Abcam, #ab221557), LDHA (1:1000, Cell Signaling, #3582S), GAPDH (1:1000, Cell Signaling,

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12/1/2015Great Human Hif2a Antibody A MThis is arguably the best antibody for human Hif2a that I have found on the market. We have been using it for years in lab, and have no reason to change our minds. With this antibody, we are able ... Read Review

HIF-2alpha (D9E3) Rabbit mAb

HIF-2alpha (D9E3) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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HIF-2alpha (D9E3) Rabbit mAb from Cell Signaling Technology

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