Fig 1: Suppression of hepatocarcinogenesis following HSF1 inactivation is accompanied by downregulation of c-Myc and its downstream effectors in the mouse liverA., B. Representative Western blot analysis of livers from control mice (injected with empty vector), c-Myc mice (injected with c-Myc), and c-Myc/HSF1dn mice (co-injected with c-Myc and HSF1dn). Inactivation of HSF1 in c-Myc/HSF1dn mice resulted in downregulation of c-Myc. Levels of c-Myc downstream effectors (FASN, ACAC, SCD1, ODC, TFAM, NRF1, HKII, LDHA/C) were downregulated in c-Myc/HSF1dn mice when compared with c-Myc mice. At least five livers from each group of mice were used for the analysis, and representative images are shown. β-Actin was used as a loading control. C.-F. Fatty acid biosynthesis, total cholesterol and triglyceride content, and lactate dehydrogenase activity were strongly induced in the livers of c-Myc mice, and equally lower in control and c-Myc/HSF1dn livers. Tukey–Kramer test: P < 0.0001 a, vs control (mice injected with empty vector); b, vs c-Myc mice. Abbreviations: FA, fatty acid; CE, cholesterol; TG, triglycerides; LDH, lactate dehydrogenase.