CD8 Antibody (YTS105.18) - BSA Free NB200-578 from Novus Biologicals, a Bio-Techne Brand

Product Specs
ItemCD8 Antibody (YTS105.18) - BSA Free
CompanyNovus Biologicals, a Bio-Techne Brand
Price $419.00Supplier Page View Company Product Page
Catalog NumberNB200-578
ImmunogenMouse spleen cells
NCBI Gene AliasesLy-35, Ly-B, CD8alpha, Ly-2, Leu2, p32, Lyt-2, CD8
Concentration1.0 mg/ml
IsotypeIgG2a
Quantity0.125 mg
Conjugate/TagUnconjugated
FormatPBS
ClonalityMonoclonal
TargetCD8
HostRat
ApplicationsIF/IHC,Flow Cytometry,Immunohistochemistry,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunohistochemistry-,Immunocytochemistry/ Immunofluorescence
ReactivityHuman,Mouse
CloneYTS105.18
NCBI Full Gene NameCD8 subunit alpha
PurityProtein G purified
Molecular Weight26 kDa
Research AreaAdaptive Immunity, Cancer, Cytokine Research, Immunology, Innate Immunity, Signal Transduction, Stem Cell Markers
Antibody TypePrimary Antibodies
StorageStore at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: CD8 Antibody (YTS105.18) recognizes a non polymorphic epitope on the mouse CD8 alpha chain. Blocks MHC I dependent T cell responses in vitro and in vivo, and induces transplantation tolerance in combination with CD4 antibodies.

Sizes Available: 0.125 mg

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Questions and Answers

Can this CD8 antibody be used for flow cytometry applications?

Yes, the CD8 Antibody (YTS105.18) has been shown to work in flow cytometry applications, as indicated by its usage in scientific literature.

What species can this antibody react with, and has it been used in any relevant studies?

This antibody is reactive with both mouse and human species. It has been utilized in studies such as those investigating the effects of human cytomegalovirus on liver development in transgenic mice, where it was employed for immunofluorescence analysis ().

Is this antibody suitable for immunohistochemistry, and what has been documented regarding its use?

The CD8 Antibody (YTS105.18) is suitable for immunohistochemistry, as evidenced by its application in studies examining hepatic steatosis in mice, where it was used for immunohistochemical staining ().

What is the immunogen used for this antibody, and how does it relate to its specificity?

The immunogen for the CD8 Antibody (YTS105.18) is derived from mouse spleen cells, which contributes to its specificity for the CD8A target in both mouse and human samples.

Novus Biologicals, a Bio-Techne Brand

United States: 10771 E Easter Ave

Centennial, Colorado 80112

United States

Phone:
303-730-1950
888-506-6887

Email: novus@novusbio.com

Company Profile

Website: https://www.novusbio.com

(1) STAT6 inhibition of M2 macrophages suppresses tumor growth by modulating the tumor microenvironment in colon cancer modelFront ImmunolJune 3, 2026Geumseong Yeun, Ik-Hwan Han, Ilseob Choi, Soyoung Kim, Hyojung Choi, Hyunsu Bae0.2% Triton X-100 for 1 hour at room temperature. The slides were then incubated with CD8 (1:200; # NB200-578, Novus Biologicals, Centennial, CO, USA) and Granzyme B (1:200; # ab255598, Abcam, Cambridge, UK) overnight

(2) Huaier Polysaccharides Sensitize Anti-PD-L1 Therapy via Promoting Antitumor Immune Response in Triple-Negative Breast CancerInt J Biol SciMay 18, 2026Lin-Xi Zhou, Zi-Wei Wu, Yuan Tian, Ke-Fei Luo, Qin-Wen Pan, Yuan-Yin Xi, Peng Tang, Ling-Juan Zeng, Ling Zhong, Jun Jiang, et al.Affinity Biosciences (Cincinnati, OH, USA). Anti-PD-L1 (NBP1-43262), LC3 (NB100-2220), CD4 (NBP1-19371), CD8 (NB200-578), FoxP3 (NB100-39002) and Ki67 (NB110-89717) antibodies were from Novus Biologicals (Littleton, CO,

(3) TIMP1 Mediates Astrocyte-Dependent Local Immunosuppression in Brain Metastasis Acting on Infiltrating CD8+ T CellsCancer discoveryJanuary 13, 2025Priego N, de Pablos-Aragoneses A, Perea-García M, Pieri V, Hernández-Oliver C, Álvaro-Espinosa L, Rojas A, Sánchez O, Steindl A, Caleiras E, et al.caspase-3 (1:500, Cell Signaling Technology, cat.# 9661), anti-CD8 (1:100, Novus Biologicals, cat.# NB200-578), osteopontin (1:100, Santa Cruz Biotechnology, cat.# 21742), and anti-MHC class 1 H2 Db/H2-D1 (1:100,

(4) Linked suppression of skin graft rejection can operate through indirect recognition"Journal of immunology (Baltimore, Md. : 1950)"January 1, 0001"Wise MP, Bemelman F, Cobbold SP, Waldmann H."

(5) Remodeling Chondroitin-6-Sulfate-Mediated Immune Exclusion Enhances Anti-PD-1 Response in Colorectal Cancer with Microsatellite StabilityCancer immunology researchJanuary 1, 0001"Wu Q, Huang Q, Jiang Y, Sun F, Liang B, Wang J, Hu X, Sun M, Ma Z, Shi Y, et al."NB300-978SS, 1:200), CD163 (Proteintech, 16646-1-AP, RRID: AB_2144924, 1:200), CD8 (Novus Biologicals, NB200-578, RRID: AB_10003082, 1:200), CD206 (Cell Signaling Technology, 91992, RRID: AB_2800175, 1:200), GLI1

(6) A novel combination immunotherapy for cancer by IL-13Rα2-targeted DNA vaccine and immunotoxin in murine tumor models"Journal of immunology (Baltimore, Md. : 1950)"January 1, 0001"Nakashima H, Terabe M, Berzofsky JA, Husain SR, Puri RK."

(7) Neonate intestinal immune response to CpG oligodeoxynucleotide stimulationPloS oneJanuary 1, 0001"Lacroix-Lamandé S, Rochereau N, Mancassola R, Barrier M, Clauzon A, Laurent F."

(8) Hypersensitivity and oral tolerance in the absence of a secretory immune systemAllergyJanuary 1, 0001"Karlsson MR, Johansen FE, Kahu H, Macpherson A, Brandtzaeg P."

(9) The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodiesEuropean journal of immunologyJanuary 1, 0001"Cobbold SP, Martin G, Waldmann H."

(10) Development of anti-PAX3 immune responses; a target for cancer immunotherapy"Cancer immunology, immunotherapy : CII"January 1, 0001"Himoudi N, Nabarro S, Yan M, Gilmour K, Thrasher AJ, Anderson J."

(11) Rats and mice exhibit distinct inflammatory reactions after spinal cord injuryThe Journal of comparative neurologyJanuary 1, 0001"Sroga JM, Jones TB, Kigerl KA, McGaughy VM, Popovich PG."

(12) STAT3 labels a subpopulation of reactive astrocytes required for brain metastasisNature medicineJanuary 1, 0001"Priego N, Zhu L, Monteiro C, Mulders M, Wasilewski D, Bindeman W, Doglio L, Martínez L, Martínez-Saez E, Cajal SRY, et al."

(13) Human Cytomegalovirus-IE2 Affects Embryonic Liver Development and Survival in Transgenic MouseCellular and molecular gastroenterology and hepatologyJanuary 1, 0001"Zhang X, Jiang S, Zhou X, Yu Z, Han S, Nan F, Qiao H, Niu D, Wang Z, Niu J, et al."NB600-1441; NOVUS, Centennial, CO), CD4 (1:200, cat. NBP1-19371; NOVUS, Centennial, CO), CD8 (1:100, cat. NB200-578; NOVUS, Centennial, CO), zonula occludens-1 (1:100, cat. A0659; ABclonal, Wuhan, China), caspase 3 (1:100,

(14) Leader cells promote immunosuppression to drive ovarian cancer progression in vivoCell reportsJanuary 1, 0001"Wilson AL, Moffitt LR, Doran BR, Basri B, Do J, Jobling TW, Plebanski M, Stephens AN, Bilandzic M."

(15) Expression of the mono-ADP-ribosyltransferase ART1 by tumor cells mediates immune resistance in non-small cell lung cancerScience translational medicineJanuary 1, 0001"Wennerberg E, Mukherjee S, Spada S, Hung C, Agrusa CJ, Chen C, Valeta-Magara A, Rudqvist NP, Van Nest SJ, Kamel MK, et al."P2X7R staining of human samples the following antibodies were used: CD8 antibody (YTS105.18) (cat # NB200–578 Novus Biologicals, 1:100) and P2X7/P2RX7 antibody (7G1D6) (NBP2–61748 Novus Biologicals, 1:100). Multiple

(16) LRG1 destabilizes tumor vessels and restricts immunotherapeutic potency"Med (New York, N.Y.)"January 1, 0001"O'Connor MN, Kallenberg DM, Camilli C, Pilotti C, Dritsoula A, Jackstadt R, Bowers CE, Watson HA, Alatsatianos M, Ohme J, et al."

(17) Elevated FBXW10 drives hepatocellular carcinoma tumorigenesis via AR-VRK2 phosphorylation-dependent GAPDH ubiquitination in male transgenic miceCell reportsJanuary 1, 0001"Lin XT, Zhang J, Liu ZY, Wu D, Fang L, Li CM, Yu HQ, Xie CM."

(18) Gut microbiota induces hepatic steatosis by modulating the T cells balance in high fructose diet miceScientific reportsJanuary 1, 0001"Zhou X, Zhang X, Niu D, Zhang S, Wang H, Zhang X, Nan F, Jiang S, Wang B."were incubated with anti-mouse CD4 (1:200, Novus, cat. NBP1-19371), anti-mouse CD8 (1:100, Novus, cat. NB200-578), anti-mouse UCP1 (1:100, ABclonal, cat. no A5857), and anti-mouse PGC-1α (1: 200, BOSTER, cat. no BA2816-1),

(19) Induction of tolerance in peripheral T cells with monoclonal antibodiesEuropean journal of immunologyJanuary 1, 0001"Qin SX, Wise M, Cobbold SP, Leong L, Kong YC, Parnes JR, Waldmann H."

(20) Involvement of intestinal epithelial cells in dendritic cell recruitment during C. parvum infectionMicrobes and infectionJanuary 1, 0001"Auray G, Lacroix-Lamandé S, Mancassola R, Dimier-Poisson I, Laurent F."

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