Fig 2: Comparisons of plasma parameters and mRNA expression of indicator of nerve regeneration in the renal arteries among the five groups. The levels of plasma renin (A), noradrenaline (B), Nog-B (C), N-terminal pro-B-type natriuretic peptide (NT-proBNP) (D) and the ratio of heart to body weight (E) in the control, heart failure (HF), renal denervation (RDN), Nog and NEP groups. The relative mRNA levels of calcitonin gene-related peptide (CGRP) (F), nerve growth factor (ß-NGF) (G), and growth-associated protein 43 (GAP-43) (H). * P < 0.05 vs. the HF and #P < 0.05 vs. the RDN group.

Fig 3: The protein expression of calcitonin gene-related peptide (CGRP), nerve growth factor (ß-NGF) and growth-associated protein 43 (GAP-43) in the renal arteries in the different groups. Western blot analysis of CGRP, GAP-43 and ß-NGF expression in the control (A), heart failure (HF) (B), renal denervation (RDN) (C), Nog (D) and NEP (E) groups and relative mRNA levels of CGRP (F), ß-NGF (G), and GAP-43 (H) in the renal arteries in the different groups. *P < 0.05 vs. the HF and # P < 0.05 vs. the RDN group.

Fig 4: Nerve regeneration was analyzed by immunofluorescence staining. The full-thickness skin wounds of control group (A) and experimental group (B) were immunostained for PGP9.5 (red), GAP43 (green) and nucleic acid was signed with DAPI (blue). Scale bar = 50 µm.