EphA2 Antibody AF3035 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemEphA2 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF3035
NCBI Gene AliasesCTPA, CTRCT6, CTPP1, ECK, ARCC2
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunocytochemistry,CyTOF-ready, Functional Assay
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Quantity100 ug (also 25 ug)
ReactivityHuman,Primate,Xenograft
TargetEphA2
HostGoat
Conjugate/TagUnconjugated
IsotypeIgG
NCBI Full Gene NameEPH receptor A2
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human EphA2, Gln25-Asn534, Accession # P29317
ConcentrationLYOPH
PurityAntigen Affinity-purified
ValidationKnockout Validated
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human EphA2 in direct ELISAs and Western blots. In direct ELISAs, less than 45% cross-reactivity with recombinant mouse EphA2 is observed and less than 1% cross-reactivity with recombinant human (rh) EphA1, rhEphA3, rhEphA4, rhEphA5, rhEphA6, rhEphA7 and rhEphA10 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Galectin-4 contributes to the maintenance of expression and activation of multiple receptor-type kinases involved in peritoneal metastasisScientific ReportsApril 16, 2026Hiroko Ideo, Miwa Umebayashi, Yoshio Takada, Akiko Tsuchidaanti-galectin-4 (AF1227), anti-EGFR (AF231), anti-IGF1R (AF305), anti-insulin R (AF1544), and anti-EphA2 (AF3035) antibodies were purchased from R&D Systems (Minneapolis, MN, USA). HRP-conjugated anti-rabbit IgG (#65-6120),

(2) Multi-receptor targeted therapy of breast cancer and brain metastases with a novel QUAD-drug conjugateBreast Cancer ResOctober 27, 2025Waldemar Debinski, Kaitlin N. Fink, John Rossmeisl, Ryan T. Mott, Kounosuke Watabe, Ralph D'Agostino, Alexandra Thomas, Denise Herpaiperformed as previously described [32]. Antibodies included EphA3 (Genetex), EphA2 (R&D Systems cat# AF3035) and IL-13RA2 (made in house). Images were acquired on an Olympus IX70 microscope with a DP80 camera

(3) Inhibitor of DNA-Binding 3 Is a Novel Regulator of Limbal Epithelial Cell Migration Via the EphA2/Akt Signaling PathwayInvestigative ophthalmology & visual scienceJuly 1, 2025Clutter ED, Yang W, Han J, Kaplan N, Peng H.dilution; Cell Signaling Technology), p-Akt S473 (4060, 1:1000 dilution; Cell Signaling Technology), EphA2 (AF3035, 1:250 dilution; R&D Systems), and p-EphA2 S897 (6347, 1:1000 dilution; Cell Signaling Technology).

(4) Differential association of EphA2 intracellular regions in biased signalingThe Journal of biological chemistryApril 1, 2025Zapata-Mercado E, Rainwater RR, Özdemir E, Azarova EV, Krzyscik MA, Pasquale EB, Hristova K.(Thermo Fisher Scientific). The following primary antibodies were used for detection: goat antihuman EphA2 (catalog no.: AF3035) from R&D Systems, rabbit anti-GFP (catalog no.: 2555) from Cell Signaling Technology, and rabbit anti-β-Actin

(5) miR-1297 is frequently downmodulated in flat epithelial atypia of the breast and promotes mammary neoplastic transformation via EphrinA2 regulationJ Exp Clin Cancer ResMarch 14, 2025Giorgia Scafetta, Gian Luca Rampioni Vinciguerra, Simona Giglio, Omar Faruq, Roberto Cirombella, Ilenia Segatto, Francesca Citron, Maria Chiara Mattevi, Elisabetta Di Renzi, Luciano Cascione, et al.being immunostained for 1 hour at room temperature using anti-EphA2 Polyclonal Goat IgG (1:100, Cat. #AF3035, R&D System, USA). The DAB detection kit (Agilent, USA) was used to produce the signal, and Mayer's

(6) Receptor Tyrosine Kinase EPHA2 Drives Epidermal Differentiation through Regulation of EGFR SignalingJournal of Investigative DermatologyAugust 1, 2024Bethany E. Perez Perez White, Calvin J. Cable, Bo Shi, Rosa Ventrella, Nihal Kaplan, Aya Kobeissi, Yuya Higuchi, Abhinav Balu, Zachary R. Murphy, Priya Kumar, et al.Northwestern University). The following antibodies were obtained from commercial sources: goat anti-EPHA2 (AF3035, immunoblot 1:250, immunofluorescence 1:50, R&D Systems), rabbit anti-EPHA2 (C-20, coimmunoprecipitation

(7) Identification of potent pan-ephrin receptor kinase inhibitors using DNA-encoded chemistry technologyProceedings of the National Academy of Sciences of the United States of AmericaMay 7, 2024Madasu C, Liao Z, Parks SE, Sharma KL, Bohren KM, Ye Q, Li F, Palaniappan M, Tan Z, Yuan F, et al.

(8) ProNGF promotes brain metastasis through TrkA/EphA2 induced Src activation in triple negative breast cancer cellsExperimental hematology & oncologyDecember 10, 2023Cicero J, Trouvilliez S, Palma M, Ternier G, Decoster L, Happernegg E, Barois N, Van Outryve A, Dehouck L, Bourette RP, et al.primary antibodies were used: anti-Claudin-5 (Invitrogen, 34–1600, rabbit), anti-EphA2 (R&D systems, AF3035, goat), anti-TrkA (Alomone, ANT-018, rabbit), anti-Src (Cell signaling, 2123, rabbit), anti-pSrc (Tyr416,

(9) Proteomics uncover EPHA2 as a potential novel therapeutic target in colorectal cancer cell lines with acquired cetuximab resistanceJournal of cancer research and clinical oncologyFebruary 1, 2023Torlot L, Jarzab A, Albert J, Pók-Udvari Á, Stahler A, Holch JW, Gerlinger M, Heinemann V, Klauschen F, Kirchner T, et al.stimulation. EPHA2-antibody treatment Cells were seeded 24 h prior to treatment with polyclonal goat anti-EPHA2 (α-EPHA2, AF3035, R&D Systems, directed against EPHA2's extracellular domain) or anti-GFP (α-GFP AF4240, R&D Systems)

(10) Growth factor independence underpins a paroxysmal, aggressive Wnt5a(High)/EphA2(Low) phenotype in glioblastoma stem cells, conducive to experimental combinatorial therapyJournal of experimental & clinical cancer research : CRApril 12, 2022Trivieri N, Visioli A, Mencarelli G, Cariglia MG, Marongiu L, Pracella R, Giani F, Soriano AA, Barile C, Cajola L, et al.,Merck – Millipore (Sigma Aldrich),Cat# L9393; RRID: AB_477163 Goat polyclonal anti EphA2,R&D System,Cat# AF3035; RRID: AB_2277943 Anti-Ki-67 antibody,Millipore,Cat# AB9260; RRID: AB_2142366 Rabbit polyclonal anti

(11) Aggressive and recurrent ovarian cancers upregulate ephrinA5, a non-canonical effector of EphA2 signaling dualityScientific reportsApril 23, 2021Jukonen J, Moyano-Galceran L, Höpfner K, Pietilä EA, Lehtinen L, Huhtinen K, Gucciardo E, Hynninen J, Hietanen S, Grénman S, et al.ImmunoWay, IF: 1:600), anti-EpCAM (#2929, Cell Signaling Technologies, IF: 1:800), anti-EphA2 ECD (AF3035, R&D Systems, immunoblot (IB): 1:1000, IF: 1:100), anti-EphA2-pS897 (#6347, Cell Signaling Technologies,

(12) MyD88/CD40 signaling retains CAR T cells in a less differentiated stateJCI insightNovember 5, 2020Prinzing B, Schreiner P, Bell M, Fan Y, Krenciute G, Gottschalk S.TBS-Tween (TBST), then incubated with primary antibodies: goat anti-human EphA2 (R&D Systems, Bio-Techne, AF3035), rabbit anti-human Bcl-2 (clone D55G8, Cell Signaling Technology), rabbit anti-human Myb (clone D2R4Y,

(13) Adaptive RSK-EphA2-GPRC5A signaling switch triggers chemotherapy resistance in ovarian cancerEMBO molecular medicineApril 7, 2020Moyano-Galceran L, Pietilä EA, Turunen SP, Corvigno S, Hjerpe E, Bulanova D, Joneborg U, Alkasalias T, Miki Y, Yashiro M, et al.antibodies against CK7 (OV‐TL 12/30, Invitrogen, MA5‐11986, WB 1:1,000, IF 1:100), EphA2 (ECD, R&D Systems, #AF3035, IF 1:100), FSP1 (AT1C3, LifeSpan Biosciences, LS‐C755562, WB 1:750), GAPDH (Sigma, Atlas Antibodies,

(14) Stemness underpinning all steps of human colorectal cancer defines the core of effective therapeutic strategiesEBioMedicineJune 1, 2019Visioli A, Giani F, Trivieri N, Pracella R, Miccinilli E, Cariglia MG, Palumbo O, Arleo A, Dezi F, Copetti M, et al.

(15) Brucine Suppresses Vasculogenic Mimicry in Human Triple-Negative Breast Cancer Cell Line MDA-MB-231BioMed research internationalJanuary 1, 2019Xu MR, Wei PF, Suo MZ, Hu Y, Ding W, Su L, Zhu YD, Song WJ, Tang GH, Zhang M, et al.Donkey anti-rabbit IgG-R (sc2095) were purchased from Santa Cruz Biotechnology (Texas, USA). Human EphA2 Antibody (AF3035) was purchased from R&D Systems (Minneapolis, MN). Antibody Anticleaved caspase-3 (9665) was purchased

(16) A Kaposi's Sarcoma-Associated Herpesvirus Infection Mechanism Is Independent of Integrins α3β1, αVβ3, and αVβ5Journal of virologySeptember 1, 2018TerBush AA, Hafkamp F, Lee HJ, Coscoy L.purchased from Calbiochem; integrin ␣V, integrin ␤7, EphA2, and EphA5 antibodies (MAB12191, MAB4669, AF3035, and MAB541, respectively) were obtained from R&D Systems; integrin ␤1 and integrin ␤3 antibodies (T2S/16

(17) Ephrin receptor A2 is a functional entry receptor for Epstein-Barr virusNature microbiologyFebruary 1, 2018Chen J, Sathiyamoorthy K, Zhang X, Schaller S, Perez White BE, Jardetzky TS, Longnecker R.blocking assay, the Detroit 562 cells growing in trans-well were pretreated with anti-EphA2 (2ug/uL, R&D system, AF3035) or anti-EphA4 (2ug/uL, Life technologies, 371600) for one hour and infected with EBV virus concentrated

(18) Engineered nanointerfaces for microfluidic isolation and molecular profiling of tumor-specific extracellular vesiclesNature communicationsJanuary 12, 2018Reátegui E, van der Vos KE, Lai CP, Zeinali M, Atai NA, Aldikacti B, Floyd FP Jr, H Khankhel A, Thapar V, Hochberg FH, et al.EGFRvIII (provided by Dr. Darrel Bigner), EGFR (AF231), hPDGFR (MAB1260), Podoplanin (AF3670), EphA2 (AF3035, R&D Systems, Minneapolis, MN), and the Cetuximab (ImClone LLC, Branchburg, NJ). Each antibody was biotinylated

(19) EphA2/Ephrin-A1 Mediate Corneal Epithelial Cell Compartmentalization via ADAM10 Regulation of EGFR SignalingInvestigative ophthalmology & visual scienceJanuary 1, 2018Kaplan N, Ventrella R, Peng H, Pal-Ghosh S, Arvanitis C, Rappoport JZ, Mitchell BJ, Stepp MA, Lavker RM, Getsios S.processed for immunofluorescence staining as previously described25 using a goat antibody against EphA2 (AF3035; R&D Systems, Minneapolis, MN, USA), rabbit polyclonal antibody against ephrin-A1 (V18; Santa Cruz Biotechnologies),

(20) Orphan G protein-coupled receptor GPRC5A modulates integrin β1-mediated epithelial cell adhesionCell adhesion & migrationSeptember 3, 2017Bulanova DR, Akimov YA, Rokka A, Laajala TD, Aittokallio T, Kouvonen P, Pellinen T, Kuznetsov SG.Cell Signaling Technologies, 1:1000); mouse anti-β actin (A2228, Sigma-Aldrich, 1:2000); goat anti-EphA2 (AF3035, R&D Systems, 1:2000); and mouse anti-GAPDH (#NB300-328, Novus Biologicals, 1:5000). Secondary antibodies

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Human EphA2 Antibody

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EphA2 Antibody

EphA2 Antibody

EphA2 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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EphA2 Antibody from R&D Systems, a Bio-Techne Brand

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